Leica CM3050 S Cryostat Instruction Manual Leica CM3050 S Cryostat English V11 02/2000 Always keep this manual near the instrument Read carefully prior to operating the instrument Important note Serial No: The information contained in this document rep- Year of Manufacture: resents state-of-the-art technology as well as the current state of knowledge Country of Origin: Federal Republic of Germany Leica will not assume liability for errors that might be contained in this manual, nor for acci- dental damage or damage arising from the de- livery, performance or use of this manual Therefore, no claims can be made based on the text and illustrations in this instruction manual Leica Microsystems Nussloch GmbH reserves the right to change technical specifications without notice since each of our products is subject to a policy of continuous improvement This document is protected under copyright laws Leica Microsystems Nussloch GmbH retains all rights related to this documentation Any reproduction of text and illustrations - or any parts thereof - in form of printing, photo- copying, microfiches, or other methods, includ- ing electronic systems, requires the prior writ- ten permission of Leica Microsystems Nussloch GmbH Issued by: The serial number and the year of manufacture Leica Microsystems Nussloch GmbH are specified on the nameplate at the back of Heidelberger Str 17 - 19 the instrument D-69226 Nussloch Germany Phone: +49 6224 143-0 Fax: +49 6224 143-200 Internet: http://wwwhbude © Leica Microsystems Nussloch GmbH Leica CM 3050 S Cryostat 3 Table of Contents 1 Important information 7 11 Symbols used in this manual and 7 12 Designated use 8 13 Qualification of personnel 8 21 General information on instrument 9 2 Safety 9 22 Integrated safety devices 9 221 Locking the handwheel 10 222 Centering the handwheel grip 10 223 Emergency stop function 11 224 Knife guard 11 23 Safety instructions: handling and operation of the instrument 12 231 Transport 12 232 Site requirements 12 233 Electrical connections 12 234 Handling microtome knives/blades 13 235 Knife guard/handwheel lock 13 236 Motorized sectioning 13 237 Defrosting/ 13 238 Frozen parts of the instrument and 13 239 Infectious/radioactive material 14 2310 Disinfection and cleaning 14 2311 Removing/reinstalling the microtome 15 2312 Display message Dry microtome 15 2313 Maintenance 15 3 Installation 16 31 Site requirements 16 311 General site requirements 16 312 Electrical connections 16 32 Unpacking and installation 16 33 Standard delivery 17 4 Instruction Manual V11 02/2000 Table of Contents 34 Installing/inserting accessories 18 341 Installing the handwheel 18 342 Inserting the accessories 18 343 The footswitch 19 35 Prior to switching on the instrument 19 4 Instrument Properties 20 41 Overview 20 42 Technical Data 22 5 Operation 24 51 Operating the instrument for the first time 24 52 Switching on/fuses 25 53 Control panel 1 26 531 Key functions in control panel 1 26 532 Display functions in control panel 1 26 533 Display indications when switching on 27 534 Menu functions: 28 535 Status display 31 536 Indication of refrigeration state, section thickness setting and counters 32 54 Control panel 2 33 541 Selecting a sectioning mode / 34 542 Setting the sectioning window 35 543 Selecting sectioning speed 36 544 Emergency stop 36 545 Coarse feed keys 37 546 Trimming and sectioning functions 38 547 Setting the section thickness 38 55 Daily operation of the instrument 39 551 Freezing specimens onto specimen 39 552 Freezing specimens onto specimen 40 553 Optional accessories for freezing 40 554 Inserting specimen discs into the 41 Leica CM3050 S Cryostat 5 Table of contents 555 Specimen orientation 41 556 Trimming / sectioning 42 56 Finishing work 43 561 Daily routine steps 43 562 Switching off the instrument for a 43 57 Defrosting 44 571 Automatic chamber defrost cycle 44 572 Manual chamber defrost cycle 45 573 Defrosting the specimen head 45 6 Troubleshooting, applications tips 46 61 Displayed error messages 46 611 Other error indications 46 62 Potential problems - causes and remedies 47 63 Reference chart for temperature settings (in minus °C) 51 7 Disinfection, cleaning and maintenance 52 71 Safety instructions 52 72 Spray disinfection with Leica Cryofect 52 73 Disinfection with conventional 53 74 Cleaning 53 75 Removing / reinstalling the microtome 54 751 Removing the microtome 54 752 Disassembling the specimen head 54 753 Reinstalling the microtome 55 76 Exchanging the fluorescent light lamp 56 8 Warranty and service 57 81 Warranty 57 82 Technical service information 57 821 Important information for repairs / returns of cryostats 58 83 Shutdown and disposal of the instrument 58 Appendix 59 6 Instruction Manual V11 02/2000 1 Important information The chapters of the Leica CM3050 S cryostat 11 Symbols used in this manual and instruction manual: their meaning Chapter 1 Structure of the manual: Table of contents Warnings Important information on this appear in a grey box and are manual marked by a warning triangle - Chapter 2 Safety Read this chapter before operating the instrument! Notes Chapter 3 Installation i-e- important user information ap- Unpacking and installation pears in a grey box and is marked Standard delivery, information by an information symbol- on accessories Chapter 4 Instrument properties Overview (5) Figures in brackets refer to item Technical data numbers in drawings or to the Chapter 5 Operation (Fig5) drawings themselves- Controls Setup, daily use and shut down Chapter 6 Trouble shooting Instrument type: and applications tips All information given in this instruction manual Operating errors applies only to the instrument type indicated on Display error messages the title page Temperature reference chart A name plate, indicating the instrument serial number, is attached to the back of the instru- Chapter 7 Disinfection, cleaning ment and maintenance Required information for all inquiries: Chapter 8 Warranty and service For any inquiries please specify: Appendix Electrohydraulic height adjustment Instrument type Ordering information accessories Serial number EC Declaration of Conformity Alphabetical index Leica CM 3050 S Cryostat 7 1 Important information General 12 Designated use This instruction manual includes important in- The Leica CM3050 S is a powerful cryostat for structions and information related to the operat- routine as well as research applications in biol- ing safety and maintenance of the instrument ogy, medicine and industry The instruction manual is an important part of the product It must be read carefully before us- The instrument has been designed for rapid ing the instrument for the first time and must al- freezing and sectioning of tissue samples ways be kept with the instrument The instrument has not been designed for un- If additional requirements, which exceed the attended storage of tissue material scope of this manual, are imposed by regula- tions and/or laws on accident prevention and environmental protection in the country of oper- The instrument may only be operated within the ation, appropriate instructions for compliance scope of its designated use as described above with such requirements must be added to this and as per the instructions given in this manual manual Any other use of the instrument is considered Read this instruction manual carefully before improper attempting to use or operate the instrument 13 Qualification of personnel Please pay particular attention to The Leica CM3050 S may only be operated by chapter 2 (safety features, safety in- trained laboratory personnel structions)- - Please read this infor- mation, even if you are already fa- All laboratory personnel designated to operate miliar with the operation and use of the instrument must carefully read the present other Leica products- instruction manual prior to starting work with the instrument 8 Instruction Manual V11 02/2000 2 Safety 21 General information on instrument 22 Integrated safety devices design and safe handling This instrument has been built and tested in ac- The instrument is equipped with the following cordance with the following safety regulations safety devices: on electrical measuring, control, regulating and laboratory devices: Handwheel lock DIN EN 292 Handwheel grip centering DIN EN 61010-1 Emergency stop function (instruments with sectioning motor only) EN 50082-1 Knife holder equipped with knife guard EN 55011 IEC 1000-4 The safety devices installed by the manufactu- rer of the instrument only constitute the basis of as well as according to the international quality accident prevention standard Mainly responsible for accident-free operation is above all the institution which owns the in- DIN ISO 9001 strument and, in addition, the designated per- sonnel who operate, service or repair the instrument In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in this in- struction manual Leica CM3050 S Cryostat 9 2 Safety 221 Locking the handwheel Instruments with sectioning motor: The sectioning motor is now blocked Always cover the cutting edge with the knife guard and lock the hand- wheel: All instruments: Prior to doing any work on The message LOCKED in the display of control knife and/or specimen- panel 1 indicates that the handwheel has been Prior to exchanging specimens- locked: During work breaks- CT-30 °C OT - 35°C The handwheel can be locked