Catalog # Aliquot Size G873-40N-10 10 µg G873-40N-100 100 µg G873-40N-1000 1 mg Recombinant Human GRO gamma (CXCL3) Recombinant human protein expressed in E. coli cells Catalog # G873-40N Lot # G3211-47 Product Description Specific Activity Recombinant Human GRO gamma (CXCL3) was expressed in E. coli cells. The protein accession number is P19876 Gene Aliases C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, GRO-gamma, Macrophage inflammatory protein 2-beta, MIP2-beta Recombinant Human Growth Regulated Oncogene Gamma (CXCL3) Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method. Determined by its ability to chemoattract 293 transfected Formulation CXCR2 cells using a concentration range of 10-100 ng/mL. Recombinant GRO-gamma/CXCL3 was lyophilized from a 0.2 µm Purity filtered concentrated (1.0 mg/mL) solution in 40 mM NaCl, 10 mM PB, pH 7.0. Recombinant Human GRO Reconstitution Protocol gamma (CXCL3) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with A quick spin of the vial followed by reconstitution in distilled water Coomassie Brilliant Blue G-250 to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. Approx. MW 8.0 kDa Storage and Stability The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six Recombinant Human GRO gamma months, with a carrier protein without detectable loss of activity. (CXCL3) Avoid repeated freeze/thaw cycles. Recombinant human protein expressed in E. coli cells Scientific Background Catalog Number G873-40N Growth regulated oncogene-gamma belongs to the family of chemotyctic Specific Lot Number G3211-47 cytokines called chemokines. It is identical with MGSA (melanoma growth Purity 95% stimulatory activity) and the new designation is CXCL3. This factor is known Stability 6 mos. at –20oC from date of shipment mainly because of its chemotactic activity. GRO expression is inducible by Storage & Shipping The lyophilized protein is stable for at serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 least one year from date of receipt at - and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In 70°C. Upon reconstitution, this cytokine certain tumor cell lines, GRO is expressed constitutively. Similar to other can be stored in working aliquots at 2° - alpha chemokines, the three GRO proteins are potent neutrophil 8°C for one month, or at -20°C for six attractants and activators. In addition, these chemokines are also active months, with a carrier protein without toward basophils. All three GROs can bind with high affinity to the IL-8 detectable loss of activity. Avoid receptor type B. repeated freeze/thaw cycles. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .