ANNUAL REPORT 1998 COLD SPRING HARBOR LABOR Ift-$1121`, 7:Wit . .1: -sr.sb COLD SPRING HARBOR LABORATORY ANNUAL REPORT 1998 ©1999 by Cold Spring Harbor Laboratory Cold Spring Harbor Laboratory P.O. Box 100 1 Bungtown Road Cold Spring Harbor, New York 11724 Website: http://www.cshl.org Managing Editor Wendy Goldstein Editorial staff Dorothy Brown, Cynthia Allen, Dawn McCoy Nonscientific Photography Margot Bennett, Ed Campodonico, Bill Dickerson, Mariana Emmons Desktop Editor Susan Schaefer Cover designer Tony Urgo Book designer Emily Harste Front cover: Female gametophytes in whole-mount ovules of Arabidopsis thaliana are visualized by enhancer-trap-driven expres- sion of a reporter gene encoding for the ri-glucoronidase (GUS) pro- tein. The enhancer-trap technique was developed at CSHL in 1995 by Rob Martienssen and Venkatesan Sundaresan, utilizing the "jumping genes" discovered by Barbara McClintock. (Image cour- tesy of Ueli Grossniklaus.) Back cover: The pond (Marlene Emmons) Section title pages: Marlene Emmons, Ed Campodonico, Bill Dickerson, Sue Lauter Contents Officers of the Corporation/Board of Trustees v Governance and Major Affiliations vi Committees vii Walter H. Page II (1915-1999) viii Jane N. Page (1918-1998)xv PRESIDENT'S ESSAY 1 DIRECTOR'S REPORT 6 Highlights10 ADMINISTRATION REPORT 41 RESEARCH 45 Tumor Viruses 46 Molecular Genetics 71 Cell Biology 105 Structure and Genomics 127 Neuroscience 151 CSHL Fellows 177 Author Index 181 THE SCHOOL OF BIOLOGICAL SCIENCES 183 Dean's Report 184 COLD SPRING HARBOR MEETINGS AND COURSES 187 Academic Affairs189 Symposium on Quantitative Biology 191 Meetings 194 Postgraduate Courses224 Seminars 274 Undergraduate Research 276 Nature Study 278 BANBURY CENTER 279 Director's Report 281 Meetings 287 DNA LEARNING CENTER 319 Director's Report 321 Workshops, Meetings, and Collaborations 333 COLD SPRING HARBOR LABORATORY PRESS 337 Publications 338 Executive Director's Report 339 FINANCE 343 Financial Statements 344 Financial Support348 Grants348 Methods of Contributing357 Capital and Program Contributions358 Nancy and Edwin Marks Imaging Center Capital Campaign 359 The School of Biological Sciences Capital Campaign 360 Annual Contributions361 LABORATORY STAFF 374 Iii Top Row: E. Harlow, W.L. Matheson, W.S. Robertson, J.J. Phelan, Jr., W.E. Murray, J.L. Spingarn, J.R. Reese, C.E. Harris, J.A. Steitz Middle Row: J. Kuriyan, L.N. Grace, W.D. Pidot, M. Ptashne, M.F. Gerry, L.B. Polsky, L.C. Quick, Jr., H.A. Dolan Bottom Row:B. Stillman, W.R. Miller, D.L. Luke Ill, E.S. Marks, J.D. Watson Not shown: C.T. Caskey, S. Hockfield, R. Jaenisch, L.M. Mendell, C.J. Sherr, H. Solomon Officers of the Corporation William R. Miller, Chairman James D. Watson, Ph.D., President Edwin S. Marks, Vice Chairman Bruce Stillman, Ph.D., Director Lola N. Grace, Treasurer G. Morgan Browne, Administrative Director Martha Farish Gerry, Secretary Whitney D. Pidot, Asst. Treasurer Board of Trustees Scientific Trustees C. Thomas Caskey, M.D., F.A.C.P. Martha Farish Gerry James L. Spingarn Merck & Co. Mill Neck, New York Great Neck, New York Edward E. Harlow, Ph.D. Lola N. Grace Howard Solomon Massachusetts General Hospital Glen Head, New York New York, New York Susan Hockfield, Ph.D. Charles E. Harris James D. Watson, Ph.D. Yale University New York, New York Cold Spring Harbor, New York Rudolf Jaenisch, Ph.D. Edwin S. Marks Honorary Trustees Massachusetts Institute of Kings Point, New York Technology Bayard Clarkson, M.D. William L. Matheson Memorial Sloan-Kettering Cancer John Kuriyan, Ph.D. Mill Neck, New York 11765 Center, New York, New York HHMI, Rockefeller University William R. Miller Robert Cummings Lorne M. Mendell, Ph.D. New York, New York Glen Head, New York State University of New York, Stony Brook William E. Murray H. Bentley Glass New York, New York Boulder, Colorado Mark Ptashne, Ph.D. Memorial Sloan-Kettering Cancer John J. Phelan Townsend J. Knight Center Mill Neck, New York New York, New York Charles J. Sherr, M.D., Ph.D. Mary D. Lindsay HHMI, St. Jude Children's Research Whitney D. Pidot Locust Valley, New York New York, New York Bruce Stillman, Ph.D. Cold Spring Harbor Laboratory Leon B. Polsky David L. Luke III New York, New York New York, New York Leslie C. Quick, Jr. Walter H. Page* Individual Trustees Palm Beach, Florida Cold Spring Harbor, New York Helen A. Dolan William S. Robertson Wendy VP. Russell Oyster Bay, New York Huntington, New York Oyster Bay, New York 'deceased Governance and Major Affiliations The Laboratory is governed by a 30-member Board of Trustees which meets three or four times a year. Authority to act for the Board of Trustees between meetings is vested in the Executive Committee of the Board of Trustees. The Executive Committee is composed of the Officers of the Board plus any other members who may be elected to the Executive Committee by the Board of Trustees. Additional standing and ad hoc committees are appointed by the Board of Trustees to provide guidance and advice in specific areas of the Laboratory's operations. Representation on the Board of Trustees itself is divided between community representatives and scientists from major educational and research institutions. The Laboratory is chartered as an educational and research institution by the Board of Regents of the Education Department of the State of New York. It is authorized to operate a graduate program under the name "Cold Spring Harbor Laboratory, Watson School of Biological Sciences" and thereat to confer the degrees of Doctor of Philosophy (Ph.D.), Master of Science (M.S.), and Doctor of Science (Sc.D.), Honorary. It is designated as a "public charity" under Section 501(c)(3) of the Internal Revenue Code. vi Committees Audit Rick Cosnotti Whitney D. Pidot Martha F. Gerry Bruce Stillman, Ph.D. Whitney D. Pidot, Chairman Laurie J. Landeau, V.M.D. William L. Matheson Mary D. Lindsay Robertson House William R. Miller David L. Luke Ill John J. Phelan Wendy Vander Poel Russell William S. Robertson, Chairman James L. Spingarn Art Brings Building James D. Watson, Ph.D. G. Morgan Browne Executive Committee Maude Robertson Helen A. Dolan, Chairman Jan Witkowski, Ph.D. Arthur Brings John P. Cleary, Esq. William R. Miller, Chairman Robertson Research Fund Martha F. Gerry G. Morgan Browne Mary D. Lindsay Lola N. Grace Carl Schafer, Chairman Jack Richards Edwin S. Marks W. Dillaway Ayres, Jr. Wendy V.P. Russell John J. Phelan G. Morgan Browne Elizabeth Watson Whitney D. Pidot Leslie C. Quick, Jr William R. Miller Bruce Stillman, Ph.D. Commercial Relations James L. Spingarn Bruce Stillman, Ph.D. James D. Watson, Ph.D. John P. Phelan, Chairman James D. Watson, Ph.D. Family Representatives G. Morgan Browne Finance and Investment John P. Cleary John P. Maroney Katherine Ernst Lola N. Grace, Chairman William R. Miller Walter Meier, M.D. G. Morgan Browne Bruce Stillman, Ph.D. William S. Robertson Charles E. Harris William D. Keen Compensation Edwin S. Marks Tenure and Appointments William R. Miller William R. Miller, Chairman William E. Murray C. Thomas Caskey, M.D. Lola N. Grace Leon B. Polsky Edward E. Harlow, Ph.D. Whitney D. Pidot William S. Robertson Susan Hockfield, Ph.D. Howard Solomon Rudolf Jaenish, Ph.D. Development John Kuriyan, Ph.D. Nominating Lorne M. Mendell, Ph.D. Leslie C. Quick, Jr. Chairman Mark Ptashne, Ph.D. W. Dillaway Ayres, Jr. Edwin S. Marks, Chairman Charles Sherr, M.D., Ph.D. G. Morgan Browne William R. Miller Bruce Stillman, Ph.D. vii Vjjj Walter Hines Page 11(1915-1999) Walter Hines Page II, whose home looked down on Cold Spring Harbor and out toward Long Island Sound, died on January 8,1999 at the age of 83. A considerate, wise, and effective leader, he spent his entire working life as a banker. Following education at St. Bernard's, Milton Academy, and Harvard University, he joined J.P. Morgan & Co. After wartime service as a naval officer on a submarine chaser in the Atlantic, he steadily moved up Morgan's ranks, becoming a vice president in 1953 and vice chairman of the board in 1968. The latter role reflected his many major successes during the 1960s as head of the company's rapidly expanding international division. In 1971, Walter was pro- moted to Morgan's presidency and was chairman of its board when he retired at the end of 1979. Walter's life was also closely associated with the Cold Spring Harbor Laboratory. Beginning in 1957, he was president of its parent body, the Long Island Biological Association, and starting in 1963, he served several long intervals on its Board of Trustees. Following his retirement from J.P. Morgan, he was for six years (1980-1986) our very able chairman. Walter's roots on Long Island date to his distinguished North Carolina-born grandfa- ther and namesake, Walter Hines Page (1855-1918). A journalist and editor, he founded with Frank Doubleday the publishing house Doubleday-Page, which in 1910 they located in Garden City, New York. Earlier in Boston, the first Walter Hines Page rose to become editor of The Atlantic Monthly and an adviser to the publishers Houghton Mifflin. Doubleday-Page achieved renown when it published Theodore Dreiser's novel Sister Carrie. The decision to publish this novel was made by Walter's grandfather, with Frank Doubleday later wanting to revoke the agreement. But its publication went ahead, albeit in a less sexually explicit form orchestrated by Dreiser's wife at the time, Sara. Soon after, Walter Hines Page founded The World's Work, a monthly magazine that placed great emphasis on the public responsibility of big business. He and Frank Doubleday also start- ed Country Life in America, more immediately a financial success.