Polish-German Symposia on Pharmaceutical Sciences 1985, May 13-15 1st Symposium Poznan – Halle, held at Poznań University of Medical Sciences 1998, October 5 2nd Polish-German Symposium in Pharmaceutical Sciences – held at Martin- Luther University Halle-Wittenberg 1999, June 24-25 3rd Polish-German Symposium “Pharmacy Before the Third Century” – held at Poznań University of Medical Sciences 2007, June 6 4th Polish-German Symposium “The Pharmacy in the New Century” - held at Martin-Luther University Halle-Wittenberg 2009, May 15-16 5th Polish-German Symposium “New Challenges for Pharmaceutical Sciences” - held at Poznań University of Medical Sciences 2011, May 20-21 6th Polish-German Symposium on Pharmaceutical Sciences “Perspectives for a new decade” – held at Heinrich-Heine University Düsseldorf 2013, May 24-25 7th Polish-German Symposium on Pharmaceutical Sciences "Interdisciplinary research for pharmacy" – held at Medical University of Gdańsk Scientific Committee Chair: Prof. Małgorzata SZNITOWSKA Members: Prof. Tomasz BĄCZEK – Gdańsk Prof. Halina EKIERT – Kraków Prof. Edmund GRZEŚKOWIAK – Poznań Prof. Renata JACHOWICZ – Kraków Prof. Roman KALISZAN – Gdańsk Prof. Cornelia KECK – Berlin Prof. Peter KLEINEBUDDE – Duesseldorf Prof. Zenon KOKOT – Poznań Prof. Rainer H. MÜLLER – Berlin Prof. Reinhard NEUBERT – Halle Prof. Christian PEIFER – Kiel Prof. Peter PROKSCH – Duesseldorf Prof. Wiesław SAWICKI – Gdańsk PREFACE Dear Colleagues, Participants of the 7th Polish-German Symposium on Pharmaceutical Sciences Polish-German meetings devoted to presentation of the achievements in pharmaceutical sciences and bilateral cooperation have been organized successfully as long as since 1985. The meetings on the Polish side were initiated by the Pharmaceutical Faculty of Poznań, joined in by the Pharmaceutical Faculties from Kraków and Gdańsk in 2007. It is an honour for us, scientists in the Faculty of Pharmacy of the Medical University of Gdańsk to welcome you in Gdansk at the 7th Polish-German Symposium on Pharmaceutical Sciences. Traditionally scientists from pharmaceutical faculties/institutes in Kiel, Düsseldorf, Halle, Berlin, Poznań and Kraków have been invited to the Symposium. Our invitation to the conference have been accepted by the large group of researchers. We can expect that a special energy will be brought by young scientists, since among 170 participants 61 are Ph.D. students. We do hope that some of them will make an effort in the future to keep going on these important neighborhood meetings for many years. The Symposium is aimed at providing a forum for high level scientific relations between German and Polish researchers working in the area of pharmaceutical sciences. This is the best chance to learn what are the current scientific achievements of the contributing schools of pharmacy and to develop better collaboration among Polish and German researches. Exceptionally friendly atmosphere is a tradition of our meetings and this must help in our future contacts. The leading topic of the symposium is „Interdisciplinary research for pharmacy”. We are sure that not only our honorary speakers, prof. Reinhard Neubert and prof. Roman Kaliszan, but also seven lecturers invited from all participating universities and authors of 164 poster presentations will show how much can be achieved in pharmaceutical sciences if broader interdisciplinary cooperation and new research tools as well as new fields of exploration are considered. The event is taking place in the history-breathing and picturesque Gdańsk. We hope that like for every Polish citizen, also for our German friends this will become a city where one wants to return many times. We wish all participants a joyful but also fruitful time here, with intensive scientific discussions and new contacts. Gdańsk, May 24-25, 2013 Prof. Tomasz Bączek Prof. Wiesław Sawicki Prof. Małgorzata Szmitowska Vice-Rector for Research Dean Chair of the Symposium Program Friday 24th May, 2013 14.00- Registration 16.00 Hanging posters at the poster boards 16.00- Opening Remarks 16.45 Plenary Lecture 16.45- Prof. Reinhard Neubert: “Interdisciplinary research in 17.15 Biopharmaceutics: Investigation of the nano-structure of the stratum corneum lipids” 17.15- Plenary Lecture 17.45 Prof. Roman Kaliszan: “Current trends in search for better drugs” 17.45- Coffee at the poster area 18.45 Saturday 25th May, 2013 Jörg Breitkreutz (Duesseldorf): “Recent advances in orodispersible 8.30- drug formulations” 9.00 9.00- Tomasz Gośliński (Poznań): “Porphyrinoid tattoo pigments with 9.30 various missions” 9.30- Coffee break 10.00 10.00- Thomas Groth (Halle): “Polysaccharide-based materials and surface 10.30 modifications for control of cell behavior” 10.30- Christian Peifer (Kiel): “Design, synthesis and biological evaluation 11.00 of kinase inhibitors” Ewa Skrzypczak-Pietraszek: “Production of therapeutically 11.00- important compounds in in vitro cultures of medicinal plants and 11.30 higher fungi 11.30- Coffee break 12.15 Visits in the Departments 12.15- Cornelia Keck (Berlin): “New developments in dermal delivery with 12.45 lipid nanoparticles and drug nanocrystals” 12.45- Krzysztof Cal (Gdansk) “Targeting to the hair follicles by antibiotic- 13.15 loaded lipospheres” 13.15- Luncheon and posters 14.15 14.15- Selected poster presentation – 3 parallel sessions * 15.15 (10 min x 5) 18.00 Departure of buses to Gdynia Gala dinner at “Dar Pomorza” 19.00 Prizes for the best posters Organizing Committee Chair: Prof. Małgorzata SZNITOWSKA Members: Department of Biofarmaceutics and Pharmacodynamics - Assoc. prof. Paweł WICZLING, Ph.D. - Damian SZCZESNY, M.Sc. Department of Pharmaceutical Technology - Prof. Krzysztof CAL - Magdalena CZAJKOWSKA, M.Sc. Department of Physical Chemistry – Piotr PIKUL, M.Sc. - Kamil WŁODARSKI, M.Sc. Department of Pharmaceutical Chemistry – Piotr KAWCZAK, Ph.D. - Ilona OLĘDZKA, Ph.D. International Relations Office - Ewa KISZKA, M.Sc. Table of Content Lectures .......................................................................................... 1-11 I - Pharmaceutical Technology ...................................................... 12-71 II - Pharmacodynamics and Biopharmacy ...................................... 72-84 III - Analysis .............................................................................. 85-129 IV – Pharmacognosy .................................................................. 130-151 V - Synthesis and Biological Activity ......................................... 152-179 VI - Pharmaceutical Practice .................................................... 180-182 Index ....................................................................................... 183-186 Lectures 1 7th Polish-German Symposium on Pharmaceutical Sciences Lectures Interdisciplinary research in Biopharmaceutics: Investigation of the nano-structure of the Stratum corneum lipids Prof. Dr. Dr. h.c. Reinhard H.H. Neubert Institute of Pharmacy, Martin-Luther-University, Halle (Saale), Germany The outermost layer of the skin, the Stratum corneum (SC) is the main penetration barrier for drugs active ingredients. The SC lipids are playing an essential role concerning drug penetration. Therefore, interdisciplinary research activities are highly necessary for the investigation of the nanostructure of the SC lipids, particularly of the ceramides, is one key research direction in order (1) to understand the barrier function of the human skin and (2) to generate new concepts for influencing dermal and transdermal drug delivery [1 - 3]. In the focus of the lecture are the influence of the ceramides on the nanostructure of the SC lipids and the search for the substantial ceramide subclasses. In the past, results were published concerning key role of the long chain ceramides concerning the barrier function of ther skin. Therefore, influence of the long chain ceramides on the nanostructure of the SC lipids is shown using the CER EOS [4, 5]. Then the impact of short chain ceramides such as CER AP und CER NP is presented. There is a change paradigm because it is shown that the short chain most polar ceramides appear to be essential for the barrier function of the SC. Based on these studies the armature reinforcement model of the SC lipid matrix was proposed: Neutron diffraction results confirm the armature reinforcement model which can be used to illustrate the nanostructure of the SC lipids [4 – 8, 11]. New results were presented in the literature to create the asymmetry model because the SC lipids form asymmetric bilayers in the SC. The short chain ceramides (e.g. the CER NP) have different long alkyl chains (C18 and C24). The asymmetric bilayers consist of one 45 nm bilayer with the C18 alkyl chain and mainly cholesterol molecules and one 65 nm bilayer with the C24 alky chain and mainly free fatty acids. CER NP appears to play a crucial role in the fully extended conformation for the nanostructure of the SC lipids. These results could be confirmed using neutron scattering experiments and a SC lipid model system as well as the Molecular Dynamic Simulation as theoretical method. Furthermore, the influence of lipophilic penetration enhancing molecules (penetration enhancer), e.g. oleic acid and isopropylmyristate on the nanostructure of the SC lipids is shown [9 – 10, 12]. A new interdisciplinary approach is presented concerning the use of the NMR spectroscopy and the lipid monolayer technique [13]. Dynamics of the ceramides inside of the SC bilayers can be studied using different NMR techniques. On the other