GARY MICHAEL WESSEL Curriculum vitae October 2020 BUSINESS ADDRESS Brown University, Box G-L168 185 Meeting Street Division of Biology and Medicine Providence, Rhode Island 02912 Phone: (401)863-1051 Fax: (401)863-1182 e-mail: [email protected] website: www.brown.edu/Research/Wessel_Lab/ Orchid ID: 0000-0002-1210-9279 EDUCATION and TRAINING Undergraduate University of Virginia, Charlottesville, Virginia B.A. 1978; Biology and Environmental Sciences Graduate Duke University, Durham, North Carolina Ph.D. 1986; Anatomy Mentors: Drs. David R. McClay and Richard B. Marchase Postgraduate University of Texas M.D. Anderson Cancer Center, Houston, Texas Department of Biochemistry and Molecular Biology NIH Postdoctoral Fellow; May 1986 - April 1989 Mentors: Drs. William H. Klein and William J. Lennarz Employment Research Assistant, Department of Zoology, Duke University 1978-1981 APPOINTMENTS • University of Texas M.D. Anderson Cancer Center; Assistant Professor of Biochemistry and Molecular Biology; May 1989 - July 1990 • Brown University, Division of Biology and Medicine; Assistant Professor of Biology; July 1990 - July 1996 • Brown University, Division of Biology and Medicine; Associate Professor of Biology; July 1996 – 2000 • Brown University, Division of Biology and Medicine; Professor of Biology; June 2000 – present • Marine Biological Laboratory, Woods Hole, MA; Senior Scientist (Adjunct); Eugene Bell Center for Regenerative Biology and Tissue Engineering, December 2005 – present Gary M. Wessel curriculum vitae October 2020 • Professor (Adjunct), Health Biotechnology Unit, Institute of Biotechnology, Addis Ababa University, Ethiopia; February 2016 - present HONORS Graduate • Research Assistantship, Department of Zoology, Duke University; 1981-1982, 1984-1986 • Graduate School Fellowship, Duke University Medical Center; 1983-1984 • Best Paper Award, Southeastern Regional Developmental Biology Conference, Lytchfield Beach, SC; 1984 • Outstanding Dissertation Award, American Association of Anatomists; 1987 Postdoctorate • NIH Postdoctoral Fellowship; 1986-1989 • University of Texas M.D. Anderson Associates Travel Award; 1988 Professorial • Fellow of the Marine Biological Laboratory, F.B. Bang Fellowship, Woods Hole, MA; 1991 • Basil O'Conner Starter Scholar Research Award, March of Dimes Birth Defects Foundation; 1992-1994 • Visiting Summer Scholar, Duke University Marine Laboratory; 1993, 1994, 1998-present • NIH Research Career Development Award, 1997-2002 • Visiting Scientist, Asamushi Marine Lab, University of Tohoku, March 1999, 2003, 2005, 2015 • Elizabeth H. LeDuc Award for Teaching Excellence in the Life Sciences, Brown University, 2003 • Astor Visiting Lectureship, University of Oxford, Oxford England, 2008 • Fellow of the American Association for the Advancement of Science (AAAS), Elected 2011 PUBLICATIONS: REFEREED JOURNALS 1. McClay, D.R., Wessel, G.M., and Marchase, R.B. (1981). Intercellular Recognition: Quantitation of Initial Binding Events. Proc. Natl. Acad. Sci. USA, 78:4975-4979. 2. Wessel, G.M., Marchase, R.B., and McClay, D.R. (1984). Ontogeny of the Basal Lamina in the Sea Urchin Embryo. Dev. Biol., 103:235-245. 3. Wessel, G.M. and McClay, D.R. (1985). Sequential Expression of Germ-Layer Specific Molecules the Sea Urchin Embryo. Dev. Biol., 111:451-463. 4. Wessel, G.M. and McClay, D.R. (1986). Two Embryonic, Tissue-Specific Molecules Identified by a Double-Label Immunofluorescence Technique for Monoclonal Antibodies. J. Histochem. Cytochem., 34:703-706. 5. Wessel, G.M. and McClay, D.R. (1987). Gastrulation in the Sea Urchin Embryo Requires the Deposition of Collagen in the Extracellular Matrix. Dev. Biol., 121:149-165. 2 Gary M. Wessel curriculum vitae October 2020 6. Wessel, G.M., Truschel, M.R., Chambers, S.M., and McClay, D.R. (1987). A Cortical Granule-Specific Enzyme, β-1,3-Glucanase in Sea Urchin Eggs. Gamete Res., 18:339-348. 7. Xiang, M., Bedard, P-A., Wessel, G.M., Filion,M., Brandhorst,B., and Klein, W.H. (1988). Tandem Duplication and Divergence of a Sea Urchin Protein Belonging to the Troponin C Superfamily. J.Biol.Chem.,263:17173-17180. 8. Decker, G.L., Valdizan, M.C., Wessel, G.M., and Lennarz, W.J. (1988). Developmental Distribution of a Cell-Surface Glycoprotein in the Sea Urchin Strongylocentrotus purpuratus. Dev. Biol.,129:339-349. 9. Wessel, G.M., Zhang, W., Tomlinson, C., Lennarz, W.J., Klein, W.H. (1989). Transcription of the Spec 1-like Gene of Lytechinus is Selectively Inhibited in Response to Disruption of the Collagenous Extracellular Matrix. Development, 106:335-347. 10. Wessel, G.M. (1989). Cortical Granule-Specific Components are Present Within Developing Oocytes and Accessory Cells during Sea Urchin Oogenesis. J.Histochem.Cytochem., 37:1409- 1420. 11. Wessel, G.M., Goldberg, L., Lennarz, W.J., and Klein, W.H. (1989). Gastrulation in the Sea Urchin Embryo is Accompanied by the Accumulation of an Endoderm-Specific mRNA. Dev. Biol. 136:526-536. 12. Wessel, G.M., Zhang, W., and Klein, W.H. (1990). Myosin Heavy Chain Accumulates in Dissimilar Cell-Types of the Macromere Lineage in the Sea Urchin Embryo. Dev. Biol. 140:447-454. 13. Gan, L., Wessel, G.M., and Klein, W.H. (1990). Regulatory Elements from Related Spec Genes of Strongylocentrotus purpuratus Yield Different Spatial Patterns with a lac Z Reporter Gene. Dev. Biol., 142: 346-359. 14. Gong, Z., Cserjesi, P., Wessel, G.M., and Brandhorst, B.P. (1991). Structure and Expression of the Polyubiquitin Gene in Sea Urchin Embryos. Mol.Rep.Dev. 28:111-118. 15. Wessel, G.M., Etkin, M., and Benson, S. (1991). Primary Mesenchyme Cells of the Sea Urchin Require an Autonomously Produced, Nonfibrillar Collagen for Skeletogenesis. Dev. Biol. 148:261-272. 16. Wessel, G.M. and Chen, S. (1993). Transient, Localized Accumulation of α-Spectrin during Sea Urchin Morphogenesis. Dev. Biol. 155:161-171. 17. Laidlaw, M. and Wessel, G.M. (1994). Cortical granule biogenesis is active throughout oogenesis in sea urchins. Development. 120: 1325-1333. 18. Wessel, G.M. (1995). A protein of the sea urchin cortical granules is targeted to the fertilization envelope and contains an LDL-receptor-like motif. Dev. Biol. 167:388-397. 3 Gary M. Wessel curriculum vitae October 2020 19. Holy, J., Wessel, G., Berg, L. Gregg, R., and Schatten, G. (1995). Molecular characterization and expression patterns of a B-type nuclear lamin during sea urchin embryogenesis. Dev. Biol. 168: 464-478. 20. Wessel, G., Clark, F., and Berg, L. (1995). A diversity of enzymes involved in the regulation of reversible tyrosine phosphorylation in sea urchin eggs and embryos. Comp. Biochm. Phys.110:493-502. 21. Wessel, G.M. and Berg, L. (1995). A spatially restricted molecule of the extracellular matrix is contributed both maternally and zygotically in the sea urchin embryo. Dev. Growth Diff. 37:517-527. 22. Chen, S.W. and Wessel, G.M. (1996). Endoderm differentiation in vitro identifies a transitional period for endoderm ontogeny in the sea urchin embryo. Dev. Biol. 175:57-65. 23. Berg, L., Chen, S.W. and Wessel, G.M. (1996). An extracellular matrix molecule that is selectively expressed during development is important for gastrulation in the sea urchin embryo. Development 122:703-713. 24. Berg, L. and Wessel, G.M. (1997). Cortical granules of the sea urchin translocate early in oocyte maturation. Development, 124:1845-1850. 25. Conner, S., Leaf, D., and Wessel, G.M. (1997). Members of the SNARE hypothesis are associated with cortical granule exocytosis in the sea urchin egg. Mol. Rep. Dev. 48:106-118. 26. Schulz, J., Wessel, G.M., and Vacquier, V. (1997). Syntaxin and VAMP are associated in sea urchin sperm and are shed in membrane vesicles during acrosomal exocytosis. Dev. Biol. 191:80- 87. 27. LaFleur, G.J., Horiuchi, Y., and Wessel, G.M. (1998). Ovoperoxidase is a member of the heme-dependent peroxidase family and is selectively expressed by developing oocytes. Mech. Dev. 70:77-89. 28. Wessel, G. M., Berg, L., Adelson, D., Cannon, G., McClay, D.R. (1998). Molecular characterization of hyalin - a cell adhesion molecule of the hyalin layer. Dev. Biol. 193:115-126. 29. Conner, S., and Wessel, G. W. (1998). Rab3 mediates cortical granule exocytosis in the sea urchin egg. Dev. Biol. 203:334-344. 30. Edelman, L., Zheng, L., Wang, Z-F., Marzluff, W., Wessel, G.M. and Childs, G. (1998). The TATA-binding protein in the sea urchin embryo is maternally derived. Dev. Biol. 204:293-304. 31. Haley, S. and Wessel, G.M. (1999). A cortical granule serine protease CGSP1 of the sea urchin Strongylocentrotus purpuratus, is autocatalytic and contains a low-density lipoprotein receptor-like domain. Dev. Biol 211:1-10. 4 Gary M. Wessel curriculum vitae October 2020 32. Conner, S. and Wessel, G. M. (1999). Syntaxin is required for cell division. Mol. Biol. Cell 10:2735-2743. 33. Moreno, R.D., Ramalho-Santos, J., Chan, E., Wessel, G.M. Schatten, G. (2000). The Golgi apparatus segregates from the lysosomal/acrosomal vesicle during Rhesus spermiogenesis: Structural alterations. Dev. Biol. 219: 334-349. 34. Gross, V.S., Wessel, G.M., Florman, H.M., and Ducibella, T. (2000) A monoclonal antibody that recognizes mammalian cortical granules and a 32 kDa protein in mouse eggs. Biology of Reproduction 63:575-81. 35. Conner, S. and Wessel, G.M. (2000). A rab3 homolog in the sea urchin functions in cell division. FASEB J 14:1559-1566 36. Ramalho-Santos, J., Moreno, R., Sutovsky, P., Chan, W-S., Hewitson, L., Wessel, G.M., Simerly, C., and Schatten, G. (2000). SNAREs in mammalian sperm: Possible implications for fertilization. Dev. Biol. 223: 54-69. 37. Sumerel, J., Morre, J., Schnackenberg, J., Nichols, J., Canman, J., Wessel, G., and Marzluff, W. (2000). Cyclin E and its associated cdk activity do not cycle during early embryogenesis of the sea urchin. Dev. Biol.234:425-440. 38. Ramalho-Santos J, Sutovsky P, Simerly C, Oko R, Wessel GM, Hewitson L, Schatten G. (2000). ICSI choreography: fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications. Hum Reprod. 12:2610-20 39. Conner, S.