DAZ2 Antibody Cat. No.: 29-234 DAZ2 Antibody Antibody used in WB on Human Daudi cells at 0.2-1 ug/ml. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human DAZ2. TESTED APPLICATIONS: ELISA, IHC, WB DAZ2 antibody can be used for detection of DAZ2 by ELISA at 1:62500. DAZ2 antibody can APPLICATIONS: be used for detection of DAZ2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1224 - Daudi Cell Lysate PREDICTED MOLECULAR 59 kDa, 65 kDa, 65 kDa, 41 kDa, 65 kDa, 65 kDa, 63 kDa, 50 kDa, 44 kDa WEIGHT: September 30, 2021 1 https://www.prosci-inc.com/daz2-antibody-29-234.html Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store DAZ2 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: DAZ2 ALTERNATE NAMES: DAZ2, pDP1678 ACCESSION NO.: NP_001005785 PROTEIN GI NO.: 54292105 GENE ID: 57055 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References DAZ2 is a member of the DAZ family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). This RNA-binding protein is important for spermatogenesis.This gene is a member of the DAZ gene family and is a candidate for the human Y- chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within BACKGROUND: palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms. REFERENCES: 1) Kuo, P.L., (2004) Fertil. Steril. 81 (4), 1034-1040. ANTIBODIES FOR RESEARCH USE ONLY. September 30, 2021 2 https://www.prosci-inc.com/daz2-antibody-29-234.html For additional information, visit ProSci's Terms & Conditions Page. September 30, 2021 3 https://www.prosci-inc.com/daz2-antibody-29-234.html.