JP XV Ultraviolet-visible Reference Spectra 1519 Acebutolol Hydrochloride A solution in 0.01 mol/L hydrochloric acid TS (1 in 100,000) Acetohexamide 1 A solution prepared as follows: Dissolve 0.10 g in 100 mL of methanol. To 5 mL of this solution add 20 mL of 0.5 mol/L hydrochloric acid TS and 75 mL of methanol (Sample solution 1). Acetohexamide 2 A solution prepared as follows: To 10 mL of Sample solution 1 add methanol to make 50 mL (Sample solution 2). 1520 Ultraviolet-visible Reference Spectra JP XV Aclarubicin Hydrochloride A solution in diluted methanol (4 in 5) (3 in 100,000) Acrinol Hydrate An aqueous solution (3 in 250,000) Actinomycin D A solution in methanol (3 in 100,000) JP XV Ultraviolet-visible Reference Spectra 1521 Afloqualone A solution in ethanol (99.5) (1 in 150,000) Alimemazine Tartrate An aqueous solution (1 in 100,000) Allopurinol An aqueous solution (1 in 200,000) 1522 Ultraviolet-visible Reference Spectra JP XV Alprazolam A solution in ethanol (95) (1 in 200,000) Alprenolol Hydrochloride A solution in ethanol (95) (1 in 10,000) Alprostadil A solution prepared as follows: To 10 mL of a solution in ethanol (99.5) (1 in 100,000) add 1 mL of potassium hydroxide-ethanol TS, and allow to stand for 15 minutes. JP XV Ultraviolet-visible Reference Spectra 1523 Alprostadil Alfadex A solution prepared as follows: To 10 mL of a solution in dilute ethanol (3 in 10,000) add 1 mL of potassium hydroxide-ethanol TS, and allow to stand for 15 minutes. Ambenonium Chloride A solution in methanol (1 in 5000) Amitriptyline Hydrochloride An aqueous solution (1 in 100,000) 1524 Ultraviolet-visible Reference Spectra JP XV Amoxapine Asolutionin0.1mol/LhydrochloricacidTS(1in50,000) Amphotericin B A solution prepared as follows: Dissolve 25 mg in 5 mL of dimethylsulfoxide, and add methanol to make 50 mL. To 1 mL of this solution add methanol to make 50 mL. Arotinolol Hydrochloride A solution in methanol (1 in 75,000) JP XV Ultraviolet-visible Reference Spectra 1525 Aspoxicillin Hydrate An aqueous solution (1 in 4000) Atenolol A solution in methanol (1 in 50,000) Azathioprine Asolutionpreparedasfollows:To5mLofasolutionin2mol/LhydrochloricacidTS(1in10,000)addwaterto make 50 mL. 1526 Ultraviolet-visible Reference Spectra JP XV Aztreonam An aqueous solution (3 in 100,000) Bacampicillin Hydrochloride A solution in methanol (1 in 1000) Baclofen Asolutionin0.1mol/LhydrochloricacidTS(1in2000) JP XV Ultraviolet-visible Reference Spectra 1527 Bamethan Sulfate A solution in 0.01 mol/L hydrochloric acid TS (1 in 10,000) Benidipine Hydrochloride A solution in methanol (1 in 100,000) Benserazide Hydrochloride Asolutionin0.1mol/LhydrochloricacidTS(1in10,000) 1528 Ultraviolet-visible Reference Spectra JP XV Benzalkonium Chloride Asolutionin0.1mol/Lhydrochloricacid(1in2000) Benzbromarone A solution in 0.01 mol/L sodium hydroxide TS (1 in 100,000) Benzethonium Chloride Asolutionin0.1mol/LhydrochloricacidTS(1in5000) JP XV Ultraviolet-visible Reference Spectra 1529 Benzylpenicillin Benzathine Hydrate A solution in methanol (1 in 2000) Benzylpenicillin Potassium An aqueous solution (1 in 1000) Berberine Chloride Hydrate An aqueous solution (1 in 100,000) 1530 Ultraviolet-visible Reference Spectra JP XV Berberine Tannate A solution prepared as follows: Dissolve 0.01 g in 10 mL of methanol and 0.4 mL of 1 mol/L hydrochloric acid TS, and add water to make 200 mL. To 8 mL of this solution add water to make 25 mL. Betahistine Mesilate Asolutionin0.1mol/LhydrochloricacidTS(1in50,000) Betamethasone A solution prepared as follows: Dissolve 1.0 mg in 10 mL of ethanol (95). To 2 mL of this solution, add 10 mL of phenylhydrazine hydrochloride TS, shake, heat in a water bath at 609C for 20 minutes, and allow it to cool. Blank: A solution obtained by proceeding in the same manner with 2.0 mL of ethanol (95). JP XV Ultraviolet-visible Reference Spectra 1531 Betamethasone Dipropionate A solution in methanol (3 in 200,000) Bezafibrate A solution in methanol (1 in 100,000) Bifonazole A solution in methanol (1 in 100,000) 1532 Ultraviolet-visible Reference Spectra JP XV Biperiden Hydrochloride An aqueous solution (1 in 2000) Bisacodyl A solution in ethanol (95) (3 in 100,000) Bleomycin Hydrochloride Asolutionpreparedasfollows:To4mgadd5mL of copper (II) sulfate TS, and dissolve in water to make 100 mL. JP XV Ultraviolet-visible Reference Spectra 1533 Bleomycin Sulfate Asolutionpreparedasfollows:To4mgadd5mL of copper (II) sulfate TS, and dissolve in water to make 100 mL. Bromazepam A solution in ethanol (99.5) (1 in 200,000) Bromhexine Hydrochloride A solution in 0.01 mol/L hydrochloric acid TS (3 in 100,000) 1534 Ultraviolet-visible Reference Spectra JP XV Bromocriptine Mesilate A solution in methanol (3 in 100,000) Bucumolol Hydrochloride An aqueous solution (1 in 60,000) Bufetolol Hydrochloride An aqueous solution (1 in 20,000) JP XV Ultraviolet-visible Reference Spectra 1535 Bufexamac A solution in ethanol (95) (1 in 100,000) Bumetanide A solution prepared as follows: Dissolve 0.04 g in 100 mL of phosphate buffer solution, pH 7.0. To 10 mL of this solution add water to make 100 mL. Bupranolol Hydrochloride Asolutionin0.1mol/LhydrochloricacidTS(1in10,000) 1536 Ultraviolet-visible Reference Spectra JP XV Butropium Bromide 1 A solution in methanol (1 in 100,000) Butropium Bromide 2 A solution in methanol (1 in 5000) Calcium Folinate An aqueous solution (1 in 100,000).