Mechanisms of Cell Death TRAIL (TNFSF10) TNF-α Death Receptor 4 (TNFRSF10A/TRAIL-R1) Death Receptor 5 Zombie Dyes (TNFRSF10B/TRAIL-R2) Propidium Iodide (PI) BAT1, TIM-4 TNF RI (TNFRSF1A) 7-Amino-Actinomycin (7-AAD) MER TNF RII (TNFRSF1B) FAS-L GAS6 (TNFSF6/CD178) TRAIL (TNFSF10) Apoptotic Cell Death Domain Zombie Dyes Phosphatidylserine K63 Ubiquitin NH2 Removal ICAM3? ROCK1 NH CD14 2 Eat-Me Signals FAS Death Inducing Cytoskeletal Rearrangement, (TNFRSF6/CD95) Signaling Complex (DISC) TRADD Cytoskeletal Rearrangement, TRADD Decoy Receptor 2 FADD (TNFRSF10D/TRAIL-R4) Actomysin Contraction Engulfment RIP1 TWEAK RIP1 oxLDL (TNFSF12) FADD CIAP1/2 K63 Ubiquitination Blebbing CD36 Death Receptor 3 TWEAK (TNFSF12) PI FADD (TNFRSF25, APO-3) 7-AAD TRAF1 FADD Procaspase 8,10 TRAF 3 Phagocyte FLIP PANX1 Macrophage Monocyte Neutrophil Dendritic Cell Fibroblast Mast Cell Procaspase 8,10 NF-kB TWEAK-R (TNFRSF12A/Fn14) Find-Me Signals Lysophosphocholine C Caspase 8,10 TRAF5 TRAF2 Sphingosine-1-Phosphate G2A? Nucleotides A Decoy TRAIL Receptor R1 (TNFRSF23) Bid Cell Survival ATP, UTP Decoy TRAIL Receptor R2 (TNFRSF22) Sphingosine-1 TRADD Phosphate Receptor Decoy Receptor 1 (TNFRSF10C/TRAIL-R3) Procaspase 3 Proliferation RIP1 G P2y2 t-Bid Bcl-2 T Chemotaxis, Caspase 3 Bcl-2-xL, MCL-1 ? ICAD RIP1 Engulfment Degradation Bax, Bak Oligomerization TRADD Death Receptor 6 Extracellular ATP Bacterial pore-forming toxins TRAIL (TNFSF10) ICAD (TNFRSF21) Monosodium urate crystals Cholesterol crystals Death Receptor DNA Fragmentation Cholera toxin B, Mitochondria Gram-negative bacteria CAD Membrane Permeability TRADD FADD Disruption FAS-L BH3 Only ROS Generation FADD DNA Fragmentation RIPK1 Necrostatin 1 (TNFSF6/CD178) Protein TLR RAIDD Activation Cytosolic Bacteria TRIF Flagellin Caspase 2 Caspase 8 Necrosome AIF, Endo G Type 3 Secretion FLIP System Rods/Needles RIPK3 Target Molecule Cleavage Caspase-2 smac/diablo Bacterial DNA Necrosulfonamide PIDDosome APAF1 (Actin, DNA, Nuclear Lamins) Intracellular LPS Bax, Bak Htra 2/Omi MLKL Procaspase 2 XIAP Membrane Rupture Cytochrome C NLRP3 p53 DAI PKR P PIDD-CC NLRC4 Caspase 3 RIPK1 Decoy Receptor 3 (TNFRSF6B) Apoptosome Severe Procaspase 3 Caspase 4, 5, 11 Virus DNA Damage Caspase 6 TRAIL (TNFSF10) Procaspase 1 Osteoprotegerin Procaspase 6 Caspase 7 (TNFRSF11B) AIM2 Procaspase 7 Caspase Oligomerization Caspase 9 ASC Focus ASC XIAP Procaspase 9 Formation XIAP IFN De novo Endoplasmic Reticulum Stress transcription DNA Damage ? Lactoferrin Survival Factor Loss Inammasome JAK-STAT Keep-Out Signal CD31 Intracellular Pyroptosis Caspase 3,7 CD46 Pore formation CD47 Phosphatidylserine (PS) Caspase 1 Cell swelling Don’t Eat Me Signals CDC50 Lysis HMGB1 ATP IL-1α IL-1β, IL-18 Lactose dehydrogenase Neutrophil Phosphatidylserine Recruitment Ca2+ Phospholipids ATP11C Xkr8 Outer LeaetLeaet Flippase Scramblase PS Exposure Annexin V Eat-Me Signal Extracellular ©BioLegend, Inc. 2014 Bcl-2 Superfamily Caspases Pro-apoptotic Anti-apoptotic Initiator Contact BioLegend Bax Bid BCL-2 A1 Caspase 2 Caspase 3 Caspase 1 biolegend.com US & Canada Toll-Free: 1.877.246.5343 (877-BIOLEGEND) Bok Bik BCL-XL BLFL-1 Caspase 8 Caspase 6 Caspase 4 US Headquarters: International: 1.858.768.5800 Bak Noxa BCL-W BCL-B Caspase 9 Caspase 7 Caspase 5 Interactive Poster: biolegend.com/celldeath San Diego, CA 92121 Bim Puma MCL-1 Caspase 10 Fax: 1.877.455.9587 Bad We would like to thank Dr. Douglas Green, St. Jude Children's Research Hospital, for his contributions to this poster. email: [email protected], [email protected] 04-0051-00.