SUBCHAPTER D—WATER PROGRAMS (CONTINUED) PART 136—GUIDELINES ESTAB- to perform the measurements required LISHING TEST PROCEDURES FOR for an application submitted to the Ad- THE ANALYSIS OF POLLUTANTS ministrator or to a State for a sewage sludge permit under section 405(f) of Sec. the Clean Water Act and for record- 136.1 Applicability. keeping and reporting requirements 136.2 Definitions. under part 503 of title 40. 136.3 Identification of test procedures. (c) For the purposes of the NPDES 136.4 Application for and approval of alter- program, when more than one test pro- nate test procedures for nationwide use. 136.5 Approval of alternate test procedures cedure is approved under this part for for limited use. the analysis of a pollutant or pollutant 136.6 Method modifications and analytical parameter, the test procedure must be requirements. sufficiently sensitive as defined at 40 136.7 Quality assurance and quality control. CFR 122.21(e)(3) and 122.44(i)(1)(iv). APPENDIX A TO PART 136—METHODS FOR OR- GANIC CHEMICAL ANALYSIS OF MUNICIPAL [72 FR 14224, Mar. 26, 2007, as amended at 77 AND INDUSTRIAL WASTEWATER FR 29771, May 18, 2012; 79 FR 49013, Aug. 19, APPENDIX B TO PART 136—DEFINITION AND 2014; 82 FR 40846, Aug. 28, 2017] PROCEDURE FOR THE DETERMINATION OF THE METHOD DETECTION LIMIT—REVISION § 136.2 Definitions. 1.11 APPENDIX C TO PART 136—DETERMINATION OF As used in this part, the term: METALS AND TRACE ELEMENTS IN WATER (a) Act means the Clean Water Act of AND WASTES BY INDUCTIVELY COUPLED 1977, Pub. L. 95–217, 91 Stat. 1566, et seq. PLASMA-ATOMIC EMISSION SPECTROMETRY (33 U.S.C. 1251 et seq.) (The Federal METHOD 200.7 Water Pollution Control Act Amend- APPENDIX D TO PART 136—PRECISION AND RE- COVERY STATEMENTS FOR METHODS FOR ments of 1972 as amended by the Clean MEASURING METALS Water Act of 1977). (b) Administrator means the Adminis- AUTHORITY: Secs. 301, 304(h), 307 and 501(a), Pub. L. 95–217, 91 Stat. 1566, et seq. (33 U.S.C. trator of the U.S. Environmental Pro- 1251, et seq.) (the Federal Water Pollution tection Agency. Control Act Amendments of 1972 as amended (c) Regional Administrator means one by the Clean Water Act of 1977). of the EPA Regional Administrators. § 136.1 Applicability. (d) Director means the director as de- fined in 40 CFR 122.2. (a) The procedures prescribed herein (e) National Pollutant Discharge Elimi- shall, except as noted in §§ 136.4, 136.5, nation System (NPDES) means the na- and 136.6, be used to perform the meas- tional system for the issuance of per- urements indicated whenever the waste constituent specified is required to be mits under section 402 of the Act and measured for: includes any State or interstate pro- (1) An application submitted to the gram which has been approved by the Director and/or reports required to be Administrator, in whole or in part, submitted under NPDES permits or pursuant to section 402 of the Act. other requests for quantitative or qual- (f) Detection limit means the minimum itative effluent data under parts 122 concentration of an analyte (sub- through 125 of this chapter; and stance) that can be measured and re- (2) Reports required to be submitted ported with a 99% confidence that the by dischargers under the NPDES estab- analyte concentration is distinguish- lished by parts 124 and 125 of this chap- able from the method blank results as ter; and determined by the procedure set forth (3) Certifications issued by States at appendix B of this part. pursuant to section 401 of the Clean Water Act (CWA), as amended. [38 FR 28758, Oct. 16, 1973, as amended at 49 (b) The procedure prescribed herein FR 43250, Oct. 26, 1984; 82 FR 40846, Aug. 28, and in part 503 of title 40 shall be used 2017] 5 VerDate Sep<11>2014 12:55 Oct 16, 2019 Jkt 247175 PO 00000 Frm 00015 Fmt 8010 Sfmt 8010 Y:\SGML\247175.XXX 247175 rmajette on DSKBCKNHB2PROD with CFR § 136.3 40 CFR Ch. I (7–1–19 Edition) § 136.3 Identification of test proce- the methods listed in these tables, the dures. provisions of 40 CFR parts 122 and 125 (a) Parameters or pollutants, for are controlling and will determine a which methods are approved, are listed permittee’s reporting requirements. together with test procedure descrip- The full texts of the referenced test tions and references in Tables IA, IB, procedures are incorporated by ref- IC, ID, IE, IF, IG, and IH of this sec- erence into Tables IA, IB, IC, ID, IE, tion. The methods listed in Tables IA, IF, IG, and IH. The year after the IB, IC, ID, IE, IF, IG, and IH are incor- method number indicates the latest porated by reference, see paragraph (b) editorial change of the method. The of this section, with the exception of discharge parameter values for which EPA Methods 200.7, 601–613, 624.1, 625.1, reports are required must be deter- 1613, 1624, and 1625. The full texts of mined by one of the standard analyt- Methods 601–613, 624.1, 625.1, 1613, 1624, ical test procedures incorporated by and 1625 are printed in appendix A of reference and described in Tables IA, this part, and the full text of Method IB, IC, ID, IE, IF, IG, and IH or by any 200.7 is printed in appendix C of this alternate test procedure which has part. The full text for determining the been approved by the Administrator method detection limit when using the under the provisions of paragraph (d) of test procedures is given in appendix B this section and §§ 136.4 and 136.5. Under of this part. In the event of a conflict certain circumstances (paragraph (c) of between the reporting requirements of this section, in § 136.5(a) through (d) or 40 CFR parts 122 and 125 and any re- 40 CFR 401.13) other additional or alter- porting requirements associated with nate test procedures may be used. 6 VerDate Sep<11>2014 12:55 Oct 16, 2019 Jkt 247175 PO 00000 Frm 00016 Fmt 8010 Sfmt 8010 Y:\SGML\247175.XXX 247175 rmajette on DSKBCKNHB2PROD with CFR rmajette on DSKBCKNHB2PROD with CFR VerDate Sep<11>2014 12:55 Oct 16,2019 Jkt247175 PO00000 Frm00017 Fmt8010 Sfmt8010 Y:\SGML\247175.XXX 247175 Environmental ProtectionAgency § 136.3 TABLE IA—LIST OF APPROVED BIOLOGICAL METHODS FOR WASTEWATER AND SEWAGE SLUDGE 1 AOAC, ASTM, Parameter and units Method EPA Standard methods USGS Other Bacteria 1. Coliform (fecal), number per 100 mL or Most Probable Num- p. 132,3 1680,11 15 9221 C E–2006. number per gram dry weight. ber (MPN), 5 tube, 1681 11 20. 3 dilution, or. Multiple tube/multiple .................................. .................................. .................................. Colilert-18®13 18 21 29. well, or. Membrane filter p. 124 3 ..................... 9222 D–2006 30 ....... B–0050–85 4. (MF) 2, single step. 2. Coliform (fecal) in presence of chlorine, MPN, 5 tube, 3 dilu- p. 132 3 ..................... 9221 C E–2006. number per 100 mL. tion, or. MF 2, single step 5 .... p. 124 3 ..................... 9222 D–2006 30. 3. Coliform (total), number per 100 mL ...... MPN, 5 tube, 3 dilu- p. 114 3 ..................... 9221 B–2006. tion, or. MF 2, single step or p. 108 3 ..................... 9222 B–2006 ........... B–0025–85 4. 7 two step. 4. Coliform (total), in presence of chlorine, MPN, 5 tube, 3 dilu- p. 114 3 ..................... 9221 B–2006. number per 100 mL. tion, or. MF 2 with enrich- p. 111 3 ..................... 9222 B–2006. ment 5. 5. E. coli, number per 100 mL 21 ................ MPN 6816 multiple .................................. 9221B.2–2006/ tube, or. 9221F–2006 12 14. multiple tube/multiple .................................. 9223 B–2004 13 ........ 991.15 10 .................. Colilert® 13 18. well, or. Colilert-18® 13 17 18 MF 2678 single step 1603 22 ..................... .................................. .................................. mColiBlue-24®19. 6. Fecal streptococci, number per 100 mL MPN, 5 tube, 3 dilu- p. 139 3 ..................... 9230 B–2007. tion, or. MF 2, or .................... p. 136 3 ..................... 9230 C–2007 ........... B–0055–85 4. Plate count ............... p. 143 3. 7. Enterococci, number per 100 mL 21 ....... MPN, 5 tube, 3 dilu- p. 139 3 ..................... 9230 B–2007. tion, or. MPN 68, multiple .................................. 9230 D–2007 ........... D6503–99 9 .............. Enterolert® 13 24. tube/multiple well, or. MF 2678 single step 1600 25 ..................... 9230 C–2007. or. Plate count ............... p. 143 3. rmajette on DSKBCKNHB2PROD with CFR VerDate Sep<11>2014 12:55 Oct 16,2019 Jkt247175 PO00000 Frm00018 Fmt8010 Sfmt8010 Y:\SGML\247175.XXX 247175 § 136.3 TABLE IA—LIST OF APPROVED BIOLOGICAL METHODS FOR WASTEWATER AND SEWAGE SLUDGE—Continued 1 AOAC, ASTM, Parameter and units Method EPA Standard methods USGS Other 8.Salmonella number per gram dry MPN multiple tube ... 1682 23. weight 11. Aquatic Toxicity 9. Toxicity, acute, fresh water organisms, Ceriodaphnia dubia 2002.0 26. LC50, percent effluent. acute. Daphnia puplex and 2021.0 26. Daphnia magna acute. Fathead Minnow, 2000.0 26. Pimephales promelas, and Bannerfin shiner, Cyprinella leedsi, acute. 8 Rainbow Trout, 2019.0 26. Oncorhynchus mykiss, and brook trout, Salvelinus fontinalis, acute. 10. Toxicity, acute, estuarine and marine Mysid, Mysidopsis 2007.0 26. organisms of the Atlantic Ocean and bahia, acute. 40 CFRCh.I(7–1–19Edition) Gulf of Mexico, LC50, percent effluent. Sheepshead Min- 2004.0 26. now, Cyprinodon variegatus, acute. Silverside, Menidia 2006.0 26. beryllina, Menidia menidia, and Menidia peninsulae, acute. 11. Toxicity, chronic, fresh water orga- Fathead minnow, 1000.0 27. nisms, NOEC or IC25, percent effluent. Pimephales promelas, larval survival and growth.