CHR Program & Abstract Book OMA TIN Chromatin STR UCTURE & FUNCTION 2006 Structure&Function Punta Cana, Dominican Republic 5 - 8 December 2006 Pr o g r am & Abstr act Book Organized By: Tony Kouzarides and Abcam Punta Cana Prog 30/10/06 10:08 Page 2 Punta Cana Prog 30/10/06 10:08 Page 1 Program & Abstract Book The third Chromatin Structure & Function Punta Cana, Dominican Republic 5 - 8 December 2006 Organizers: Tony Kouzarides (University of Cambridge) and Abcam Table of contents Conference Program . .Page 2 Poster Index . .Page 6 Abstracts - Oral . .Page 18 Abstracts - Poster . .Page 51 Resort Information . .Page 179 Disclaimer: Material contained within this booklet should be citied only with permission from the author(s). No live recording or photography is permitted during the oral or poster sessions. Copyright © 2006 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print. 1 Punta Cana Prog 30/10/06 10:08 Page 2 Chromatin Structure & Function Punta Cana, Dominican Republic, 5 - 8 December 2006 Conference Program Tuesday 5th December Meeting room - Allegro Plaza Keynote Speaker - introduced by Tony Kouzarides 18:00 - 19:00 Steve Henikoff . .Page 18 Epigenetic patterns generated by histone replacement Welcome reception and buffet at poolside. Allegro Live resort show Wednesday 6th December Chair: Jerry Workman 09:00 - 09:30 Yang Shi . .Page 19 The identification of histone demethylases established the dynamic and reversible nature of histone methylation regulation 09:30 - 09:45 Jesper Christensen . .Page 20 The retinoblastoma tumor suppressor binding protein RBP2 is a transcriptional repressor demethylating tri- and dimethylated lysine 4 on Histone H3 09:45 - 10:00 Mischa Machius . .Page 21 Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase 10:00 - 10:30 Ramin Shiekhattar . .Page 22 Functional and biochemical characterization of histone demethylase complexes Drinks break in hotel lobby 11:00 - 11:30 Yi Zhang . .Page 23 Histone demethylation by the JmjC domain-containing proteins 11:30 - 11:45 Francis Stewart . .Page 24 Epigenetic aspects of lineage commitment 2 Punta Cana Prog 30/10/06 10:08 Page 3 Conference Program 11:45 - 12:00 Henk Stunnenberg . .Page 25 Title and abstract unavailable 12:00 - 12:30 Ali Shilatifard . .Page 26 H2B monoubiquitination and H3K4 methylation via COMPASS Lunch at the Beach Buffet Restaurant and free time Chair: Yang Shi 16:00 - 16:30 Paulo Sassone-Corsi . .Page 27 A chromatin remodeling clock 16:30 - 16:45 Sung Hee Baek . .Page 28 A Novel Link between SUMO Modification of a Chromatin Remodeling Complex and Cancer Metastasis 16:45 - 17:00 Laszlo Tora . .Page 29 The simultaneously dimethylated Lys-9 and phosphorylated Ser-10 tails of histone H3 adopt different conformations during mitosis 17:00 - 17:30 Tony Kouzarides . .Page 30 Characterisation of novel histone modifications 18:00 - 21:00 Posters and buffet by the pool Allegro Live resort show Thursday 7th December Chair: Genevieve Almouzni 09:00 - 09:30 Thomas Jenuwein . .Page 31 Epigenetic control by histone methylation 09:30 - 09:45 Roberta Benetti . .Page 32 The role of Dicer in the regulation of chromatin at telomeres 3 Punta Cana Prog 30/10/06 10:08 Page 4 Chromatin Structure & Function Punta Cana, Dominican Republic, 5 - 8 December 2006 09:45 - 10:00 Mareike Puschendorf . .Page 33 Ezh2 independent targeting of PRC1 proteins to paternal constitutive heterochromatin in mouse pre-implantation embryos 10:00 - 10:30 Edith Heard . .Page 34 The nuclear and epigenetic dynamics of X-chromosome inactivation in the mouse Drinks break in hotel lobby 11:00 - 11:30 Adrian Bird . .Page 35 MeCP2: molecular interactions and phenotypic stability in a mouse model of Rett Syndrome 11:30 - 11:45 Jon Penterman . .Page 36 DNA demethylation in Arabidopsis thaliana 11:45 - 12:00 Francois Fuks . .Page 37 The Polycomb Group protein EZH2 is recruited to promoters by MECP2 12:00 - 12:30 Shelley Berger . .Page 38 Factor and histone covalent modifications in genome regulation Lunch at the Beach Buffet Restaurant and free time Chair: Ramin Shiekhattar 16:00 - 16:30 Danny Reinberg . .Page 39 A molecular understanding of epigenetics 16:30 - 16:45 Jessica Tyler . .Page 40 The mechanistic basis for the requirement of promoter chromatin disassembly for transcriptional activation 16:45 - 17:00 Gratien Prefontaine . .Page 41 Epigenetic mechanisms influencing pituitary gene expression 17:00 - 17:30 Bob Kingston . .Page 42 Possible roles in silencing for piRNAs 4 Punta Cana Prog 30/10/06 10:08 Page 5 Conference Program 18:00 - 19:30 Posters and drinks by the pool Beach barbeque and live band on the sand Friday 8th December Chair: Edith Heard 09:00 - 09:30 Michael Grunstein . .Page 43 Deacetylation of histone H4 K16 regulates gene activity in yeast 09:30 - 09:45 Ann Ehrenhofer-Murray . .Page 44 A role for the HDAC Rpd3 in establishing eurchromatin- heterochromatin boundaries at yeast telomeres 09:45 - 10:00 Wyatt Yue . .Page 45 CARM1 and histone methylation - a structural study 10:00 - 10:30 Sharon Dent . .Page 46 Common and unique factors regulate Set1-mediated methylation of the Dam1 kinetochore protein and histone H3 Drinks break in hotel lobby 11:00 - 11:30 Genevieve Almouzni . .Page 47 Chromatin assembly factors, histone H3 variants and cell cycle 11:30 - 11:45 Dmitry Fyodorov . .Page 48 ATP-dependant deposition of Histone H3.3 by Drosophila CHD1 in vivo 11:45 - 12:00 Roberto Mantovani . .Page 49 The histone fold trimer NF-Y is required to define positive histone marks in CCAAT-promotors: a genome wide analysis 12:00 - 12:30 Jerry Workman . .Page 50 Histone modification and chromatin remodeling in transcription Lunch at the Beach Buffet Restaurant Conference ends 5 Punta Cana Prog 30/10/06 10:08 Page 6 Chromatin Structure & Function Punta Cana, Dominican Republic, 5 - 8 December 2006 Poster Index Abstract P1 Karl Agger . .Page 51 The role of the polycomb group protein RYBP in oncogene induced senescence Abstract P2 Helena Ahlfors . .Page 52 A novel player in T helper cell differentiation Abstract P3 Barbara Alberter . .Page 53 Histone modification pattern of the T lymphotropic Herpesvirus saimiri genome in latency Abstract P4 Marco Alvarez . .Page 54 Histone variant macroH2A is an epigenetic factor involved in the modulation of ribosomal gene expression during seasonal adaptation of carp fish Abstract P5 Terra Arnason . .Page 55 Rsp5 is required for nuclear shuttling of the Snf1 kinase complex in yeast Abstract P6 Stuart Atkinson . .Page 56 Epigenetic mechanisms of pluripotency and differentiation Abstract P7 Joanne Attema . .Page 57 Epigenetic features of hematopoietic stem cells using small numbers of highly purified primary cells Abstract P8 Kristin Baetz . .Page 58 NuA4 is a cellular “Hub”: an integrative map of physical and genetic interactions mediated by the NuA4 histone acetyltransferase Abstract P9 Slobodan Barbaric . .Page 59 Chromatin remodeling activities at the yeast PHO84 promoter Abstract P10 Vivian Bardwell . .Page 60 Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets Abstract P11 Amrita Basu . .Page 61 Computational prediction of histone and non-histone proteins 6 Punta Cana Prog 30/10/06 10:08 Page 7 Poster Index Abstract P12 Mark Bedford . .Page 62 Screening for the methylated proteome Abstract P13 Sukesh R. Bhaumik . .Page 63 Regulation of transcriptional activation by mRNA cap-binding complex in vivo Abstract P14 Marjorie Brand . .Page 64 The Ash2L/MLL2 methyltransferase complex is important for ß- globin transcription during erythroid differentiation Abstract P15 Lauren Buro . .Page 65 Histone methylation patterns at interferon-gamma inducible gene loci Abstract P16 Jill Butler . .Page 66 CXXC-finger protein 1 regulates Dnmt1 protein expression Abstract P17 Jim Cakouros . .Page 67 Identification of a novel enzyme which regulates the kinetics of histone arginine methylation in Drosophila melanogaster Abstract P18 Raymond Camahort . .Page 68 Genome-wide analysis of the budding yeast histone variant Cse4 reveals occupancy at a single centromeric nucleosome as well as additional non-centromeric locations Abstract P19 Dylan Carney . .Page 69 The RAG2 PHD Finger links the histone code to V(D)J recombination Abstract P20 Beverly Chilton . .Page 70 Analysis of RUSH/SMARCA3 isoforms and their interactions with Egr-1 and c-Rel in the regulation of transcription Abstract P21 Alexandra Chittka . .Page 71 Signalling by a novel p75 neurotrophin receptor interacting protein, SC1/PRDM4 Abstract P22 Leslie Chu . .Page 72 Inheritance of epigenetic chromatin states 7 Punta Cana Prog 30/10/06 10:08 Page 8 Chromatin Structure & Function Punta Cana, Dominican Republic, 5 - 8 December 2006 Abstract P23 Mair Churchill . .Page 73 Structural basis for the histone chaperone activity of Asf1 Abstract P24 Jeffrey Craig . .Page 74 What makes centromeres localise and cluster in interphase nuclei? Abstract P25 Valerie Crusselle-Davis . .Page 75 Regulation of beta-globin expression through the recruitment of chromatin modifying enzymes by TFII-I and USF Abstract P26 Eullia de Nadal . .Page 76 Control of gene expression by the yeast Hog1 MAPK. Abstract P27 Foteini Davrazou . .Page 77 Molecular mechanism of histone H3K4me3 recognition by the PHD finger of ING2 Abstract P28 Roger Deal . .Page 78 Repression of flowering in Arabidopsis requires histone H2A.Z deposition by a putative SWR1 complex Abstract P29 Laurent Delva . .Page 79 The Transcription Intermediary Factor 2 is required for zebrafish development Abstract P30 Luisa Di Stefano . .Page 80 Lsd1 mutation in Drosophila disrupt normal level of H3K4 methylation and affects viability and fertility Abstract P31 Stephan Diekmann . .Page 81 In vivo dynamic (FRAP, FCS) and neighbourhood relation (AB- FRET, FLIM) studies of human inner kinetochore proteins Abstract P32 Jeffrey Dilworth . .Page 82 MEF2 helps establish muscle specific pattern of gene expression by recruiting Trithorax Group proteins to specific promoters Abstract P33 Ivana Djuretic .