Product datasheet Anti-NOX5 Antibody Catalog Number: A03090 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-NOX5 Antibody Gene Name NOX5 Source Rabbit IgG Species Reactivity human Tested Application WB,FCM,Direct ELISA,ICC/IF Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E. coli-derived human NOX5 recombinant protein (Position: E13-F197). Purification Immunogen affinity purified. Observed MW 86KD Dilution Ratios Western blot: 1:500-2000 Flow Cytometry: 1-3μg/1x106 cells Direct ELISA： 1:100-1000 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. It is found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT- PCR and Southern and Western blot analyses, NOX5 was identified as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells. Reference Anti-NOX5 Antibody被引用在1文献中。 CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells | Jafari N, Kim H, PubMed:28099519 Park R, Li L, Jang M, Morris AJ, Park J, Huang C. | PLoS One. 2017 Jan 18;12(1):e0170327. doi: 10.1371/journal.pone.0170327. eCollection 2017. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-NOX5 Antibody Catalog Number: A03090 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Selected Validation Data Figure 1. Western blot analysis of NOX5 using anti-NOX5 antibody (A03090). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human placenta tissue lysates,Lane 3: human SGC-7901 whole cell lysate,Lane 4: human Jurkat whole cell lysate,Lane 5: human THP-1 whole cell lysate. probed with a goat anti- rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NOX5 at approximately 86KD. The expected band size for NOX5 is at 86KD. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Powered by TCPDF (www.tcpdf.org).