10th INTERNATIONAL FOAMY VIRUS CONFERENCE 2014 June 24‐25, 2014 National Veterinary Research Institute, Pulawy, Poland Organized by: National Veterinary Research Institute Committee of Veterinary Sciences Polish Academy of Sciences Topics include: Foamy Virus Infection and Zoonotic Aspects, Foamy Virus Restriction and Immunity, Foamy Virus Biology and Gene Therapy Vectors http://www.piwet.pulawy.pl/ifvc/ Organizers Jacek Kuźmak and Magdalena Materniak Scientific Advisors Martin Löchelt and Axel Rethwilm Sponsors Support for the meeting was provided by the Committee of Veterinary Sciences Polish Academy of Sciences, ROCHE, Thermo Fisher Scientific and EURx Abstract book was issued thanks to the support provided by Committee of Veterinary Sciences Polish Academy of Sciences 2 Program Overview Monday, June 23rd, 2014 19 00 – 20 00 Registration 20 30 – open end Welcome Reception Tuesday, June 24th, 2014 9 00 – 9 30 Registration 9 30 – 9 45 Welcome 9 45 – 10 15 Arifa Khan (FDA, Bethesda, US) - Simian foamy virus infection in natural host species 10 15 – 10 45 Antoine Gessain (Institute Pasteur, Paris, France) – Zoonotic human infection by simian foamy viruses 10 45 – 11 20 Session 1 on Foamy Virus Infection and Zoonotic Aspects Chairs: Arifa S. Khan and Antoine Gessain 11 20 – 11 45 Coffee break 11 45 – 12 30 Session 1 on Foamy Virus Infection and Zoonotic Aspects Chairs: Arifa S. Khan and Antoine Gessain 12 30 – 13 00 Aris Katzourakis (Oxford University, UK - Mammalian genomes, their endogenous viral elements, and the evolutionary history of the foamy viruses 00 15 13 – 14 Lunch Break 14 15 – 14 45 Dirk Werling (Royal Veterinary College, London, UK) - Messing up interferons – how viruses impact on IFN signalling 14 45 – 15 45 Session 2 on Foamy Virus Restriction and Immunity Chairs: Dirk Werling and Martin Löchelt 15 45 – 16 05 Coffee break 16 05 – 16 45 Rafal Kochanowski (ROCHE, Poland) – The future of laboratory developed testing – The FLOW system powered by Roche Expand your potential today 18 00 Trip to Kazimierz Dolny and sightseeing 20 30 Dinner at Sarzyński Restaurant 3 Wednesday, June 25th, 2014 9 30 – 10 10 Dirk Lindemann (TU Dresden, Germany) – Foamy Virus Structural protein Biosynthesis and Particle egress: A Personal Perspective 10 10 – 11 10 Session 3 on Foamy Virus Replication, Proteins and Assembly Chairs: Dirk Lindemann and Wentao Qiao 11 10 – 11 30 Coffee Break 11 30 – 13 00 Session 3 on Foamy Virus Replication, Proteins and Assembly 13 00 – 14 15 Lunch Break 14 15 – 14 50 Yahia Chebloune (University Joseph Fourier, Grenoble, France) – Retroviral vector for gene transfer : are we ready to move on Foamy? 14 50 – 15 10 Session 4 on FV vectors Chair: Yahia Chebloune 15 10 – 15 30 Arifa Khan (FDA, Bethesda, US) - FVs Taxonomy 15 30 – 16 30 Round-table Discussion on FVs Taxonomy and Prototype FV Moderators: Arifa Khan, Martin Löchelt, Antoine Gessain, Aris Katzourakis and Dirk Lindemann 16 30 Concluding Remarks 18 00 Barbecue in Janowiec castle 4 Program Monday, June 23rd, 2014 19 00 – 20 00 Registration 20 30 – open end Welcome Reception Tuesday, June 24th, 2014 9 00 – 9 30 Registration 9 30 – 9 45 Welcome 9 45 – 10 15 Arifa Khan (FDA, Bethesda, US) - Simian foamy virus infection in natural host species 10 15 – 10 45 Antoine Gessain (Institute Pasteur, Paris, France) – Zoonotic human infection by simian foamy viruses 10 45 – 11 20 Session 1 on Foamy Virus Infection and Zoonotic Aspects Chairs: Arifa S. Khan and Antoine Gessain Abst.# 1. Humans infected with SFV from chimpanzee and gorilla clades generate neutralizing antibodies targeting antigenic determinants shared by both SFV clades Caroline Lambert, Julie Gouzil, Réjane Rua, Edouard Betsem, Antoine Gessain and Florence Buseyne 2. High genetic variability of the envelope gene of foamy viruses from Gorilla and hunters infected by zoonotic strains Léa Richard, Rejane Rua, Philippe V. Afonso, Edouard Betsem, Antoine Gessain 11 20 – 11 45 Coffee break 11 45 – 12 30 Session 1 on Foamy Virus Infection and Zoonotic Aspects Chairs: Arifa S. Khan and Antoine Gessain Abst.# 3. Screening for equine foamy virus infections in horses Magdalena Materniak, Jerzy Rola, Wojciech Rożek, Aneta Pluta, Martin Löchelt, Jacek Kuźmak 5 4. The expression of bovine foamy virus in experimentally inoculated calves Piotr Kubiś, Magdalena Materniak, Martin Löchelt, Jacek Kuźmak 5. Seroreactivity to non-primate foamy viruses in patients immunosupressed after kidney transplantation Magdalena Materniak, Agnieszka Serwacka, Andrzej Rydzewski, Sławomir Rudzki, Łukasz Bocian, Timo Kehl, Martin Löchelt, Jacek Kuźmak 12 30 – 13 00 Aris Katzourakis (Oxford University, UK - Mammalian genomes, their endogenous viral elements, and the evolutionary history of the foamy viruses 13 00 – 14 15 Lunch Break 14 15 – 14 45 Dirk Werling (Royal Veterinary College, London, UK) - Messing up interferons – how viruses impact on IFN signalling 14 45 – 15 45 Session 2 on Foamy Virus Restriction and Immunity Chairs: Dirk Werling and Martin Löchelt Abst. # 6. N-myc interactor inhibits prototype foamy virus by sequestering viral Tas protein in the cytoplasm Xiaomei Hu, Wei Yang, Ruikang Liu, Yunqi Geng Wentao Qiao, Juan Tan 7. Knockdown of the host cellular protein transportin 3 (TNPO3) attenuates prototype foamy virus infection Md. K. Ali, Faysal B. Hamid, Jin Sun Kim, Cha-Gyun Shin 8. Activation of epitope-specific CTLs using feline foamy virus vectors Janet Lei, Wolfram Osen, Agnes Hotz-Wagenblatt, Stefan Eichmüller, Martin Löchelt 15 45 – 16 05 Coffee break 05 45 16 – 16 Rafal Kochanowski (ROCHE, Poland) – The future of laboratory developed testing – The FLOW system powered by Roche Expand your potential today 18 00 Trip to Kazimierz Dolny and sightseeing 20 30 – open Dinner at Sarzyński Restaurant end 6 Wednesday, June 25th, 2014 30 10 9 – 10 Dirk Lindemann (TU Dresden, Germany) – Foamy Virus Structural protein Biosynthesis and Particle egress: A Personal Perspective 10 10 – 11 10 Session 3 on Foamy Virus Replication, Proteins and Assembly Chairs: Dirk Lindemann and Wentao Qiao Abst.# 9. Nuclear trafficking of foamy virus Joris Paris, Marie-Louise Giron, Antoine Bridier-Nahmias, Pascale Lesage, Philippe Roingeard, Joëlle Tapiero-Tobaly, Ali Saïb 10. The cooperative function of arginine residues in the prototype foamy virus Gag C-terminus mediates viral and cellular RNA encapsidation Martin V. Hamann , Erik Müllers, Juliane Reh, Nicole Stanke, Dirk Lindemann 11. Importance of prototype-foamy virus Gag interaction with cellular polo-like kinases Irena Zurnic, Sylvia Hütter, Roger Helbig, Ute Lehmann, Gesche Gerresheim, Dirk Lindemann 11 10 – 11 30 Coffee Break 11 30 – 13 00 Session 3 on Foamy Virus Replication, Proteins and Assembly Chairs: Dirk Lindemann and Wentao Qiao 12. The non-overlapping role of N-terminal residues of feline foamy virus Gag during viral assembly and budding Yang Liu, Matthew Betts, Janet Lei, Qiuying Bao, Guochao Wei, Axel Szabowski, Timo Kehl, Robert Russell and Martin Löchelt 13. Molecular characterization of particle release phenotype of bovine foamy viruses Qiuying Bao, Yang Liu, Michaela Hipp, Birgit Hub, Timo Kehl, Wentao Qiao and Martin Löchelt 7 14. Residue N108 of bovine foamy virus transactivator Tas contributes to its binding with LTR and IP promoters Tiejun Bing, Suzhen Zhang, Peng Shao, Song Zhang, Juan Tan, Wentao Qiao 15. Detection of highly expressed RNA polymerase III-directed retroviral microRNAs of bovine foamy virus Adam W. Whisnant, Timo Kehl, Qiuying Bao, Magdalena Materniak, Jacek Kuźmak, Martin Löchelt and Bryan R. Cullen 13 00 – 14 15 Lunch Break 14 15 – 14 50 Yahia Chebloune (University Joseph Fourier, Grenoble, France) – Retroviral vector for gene transfer : are we ready to move on Foamy? 14 50 – 15 10 Session 4 on FV vectors Chair: Yahia Chebloune Abst. # 16. Foamy virus vectors for mesenchymal stem cell based gene therapy Nathan P Sweeney,Cathy Regan, Jiahiu Liu, Begona Cachon Gonzalez, Dirk Lindemann, Tony Rupar, Timothy Cox, Myra McClure 15 10 – 15 30 Arifa Khan (FDA, Bethesda, US) - talk on FVs Taxonomy 15 30 – 16 30 Round-table Discussion on FVs Taxonomy and Prototype FV (moderators: Arifa Khan, Martin Löchelt, Antoine Gessain, Aris Katzourakis and Dirk Lindemann) 16 30 Concluding Remarks 18 00 - open end Barbecue in Janowiec castle 8 Session 1 Foamy Virus Infection and Zoonotic Aspects Chairs: Arifa S. Khan and Antoine Gessain Abst.# 1. Humans infected with SFV from chimpanzee and gorilla clades generate neutralizing antibodies targeting antigenic determinants shared by both SFV clades Caroline Lambert, Julie Gouzil, Réjane Rua, Edouard Betsem, Antoine Gessain and Florence Buseyne 2. High genetic variability of the envelope gene of foamy viruses from Gorilla and hunters infected by zoonotic strains Léa Richard, Rejane Rua, Philippe V. Afonso, Edouard Betsem, Antoine Gessain 3. Screening for equine foamy virus infections in horses Magdalena Materniak, Jerzy Rola, Wojciech Rożek, Aneta Pluta, Martin Löchelt, Jacek Kuźmak 4. The expression of bovine foamy virus in experimentally inoculated calves Piotr Kubiś, Magdalena Materniak, Martin Löchelt, Jacek Kuźmak 5. Seroreactivity to non-primate foamy viruses in patients immunosupressed after kidney transplantation Magdalena Materniak, Agnieszka Serwacka, Andrzej Rydzewski, Sławomir Rudzki, Łukasz Bocian, Timo Kehl, Martin Löchelt, Jacek Kuźmak 9 Abstract 1 HUMANS INFECTED WITH SFV FROM CHIMPANZEE AND GORILLA CLADES GENERATE NEUTRALIZING ANTIBODIES TARGETING ANTIGENIC DETERMINANTS SHARED BY BOTH SFV CLADES Caroline