Full Schedule ILANIT 20th-23rd of February 2017 MONDAY, FEBRUARY 20, 2017 10:45-15:30 Registration and Refreshments - Dan hotel 15:30-16:00 Opening Ceremony -Herods hotel – Pavilion 16:00-17:00 PL1: PLENARY LECTURE: Regulation of microRNA Herods hotel - Pavilion Narry Kim is a Professor at Seoul National University and a founding director of RNA Research Center at Institute for Basic Science. Kim received her Ph.D. in 1998 from Oxford University on virology, and studied mRNA surveillance as postdoc at the University of Pennsylvania. Her group at SNU has been investigating how microRNAs are made and regulated, and what RNAs and RNA-binding proteins do to control cell fates. Kim received L’Oreal-UNESCO Women in Science (2008) and the Ho-Am Prize in Medicine (2009), and was elected as Foreign Associates of National Academy of Science and of European Molecular Biology Organization. Sponsored by: I-CORE Gene Regulation in Human Complex Disease 17:30-18:30 PL2: PLENARY LECTURE: Being at the Base of the Food Chain May Not Be So Bad After All Herods hotel - Pavilion Prof. Ian T. Baldwin received an AB from Dartmouth College in 1981, his PhD from Cornell University in 1989, rose through the academic ranks at the State University of New York at Buffalo and in 1996 became the founding director of the Max Planck Institute for Chemical Ecology in Jena, Germany, where he heads the Department of Molecular Ecology. He is a member of the US National Academy of Sciences, European Molecular Biology Organization (EMBO), Nationale Akademie der Wissenschaften Leopoldina, Berlin Brandenburgische Akademie der Wissenschaften, Wissenschaftskolleg Berlin, and has published 430 peer-reviewed papers and one book on the Induced defenses of plants. Sponsored by: I-CORE Plant Adaptation to Changing Environment 19:00-23:00 Opening Reception Dinner - Dan hotel Pool Area 2 TUESDAY, FEBRUARY 21, 2017 07:30-08:30 EBT1: Early bird session of Eldan Electronic Instruments Co.Ltd., Herods hotel - Kings hall A Session participants will be enrolled in a lottery having the chance to win a tablet. 08:30 CRISPR Guide RNA Libraries for Functional Genomics Screening: Do You Know What’s in Your Library? Dr. Carsten Carstens Agilent Technologies, Eldan Electronic Instruments Co.Ltd. Screening of CRISPR libraries, either as a knock-out or as a CRISPR A/I approach, has become a very powerful tool for functional genomics approaches to gene function discovery. The quality of the library screen is strongly dependent on the quality of the input library. Based on Agilent’s capability to synthesize up to 2.44 x 105 high quality defined DNA oligomers of 230 nt in length in a single reaction, we have developed novel chemistries, and novel, overlap-based cloning method and workflows necessary for generating high quality, low-bias plasmid libraries. The session will present the procedures and tools required to generate highest quality plasmid libraries from complex oligomer pools. 08:00 Integrated Tools for Sensitive NGS Analysis of ccfDNA Katja Schreiter Ph.D., Market Development Manager BRC & NGS Commercial Partners EMEA QIAGEN, Eldan Electronic Instruments Co.Ltd. Next generation sequencing analysis of free circulating DNA from plasma has quickly been adopted for cancer testing as well as for noninvasive prenatal testing (NIPT). For cancer patients, this method enables the noninvasive diagnosis of actionable mutants to select a personalized anti-tumor therapy and could be used to monitor molecular changes upon cancer treatment or during tumor progression. The concentration of free circulating DNA in liquid biopsies usually is very low, which makes it very important to apply optimized protocols for cfDNA extraction as well as NGS DNA library construction to successfully establish robust test systems. In this scientific talk, we will introduce new technical solutions for Ion Torrent as well as Illumina sequencing platforms that combine optimized cfDNA isolation, highly advanced library construction, unbiased library amplification and integrated data analysis to sensitively and reliably analyze cfDNA samples. 3 TUESDAY, FEBRUARY 21, 2017 07:30-08:30 EBT2: Early Bird session of Zotal Herods hotel - Kings hall B Session participants will be enrolled in a lottery having the chance to win a tablet. 07:30 RNA-Seq as an Efficient Tool for Gene Expression Profiling QuantSeq: High Multiplexing, Sensitivity, and Reproducibility with Lowest Costs and Efforts Birgit Steinmetz Product Manager at Lexogen-Zotal With the discontinuation of microarray systems and the rapid development of NGS technologies, and declining sequencing costs, attention is now on RNA-Seq as the method-of- choice for gene expression (GEX) profiling. Lexogen’s QuantSeq 3’ mRNA-Seq system offers a comprehensive and highly affordable solution for whole transcriptome GEX analyses: With a price from USD 19.80 per library prep and sequencing costs from USD 5.00 per sample, easy handling, and an all-in-one express protocol that is suitable for high throughput screening QuantSeq enables exceptionally precise and cost-effective gene expression experiments. Since only 1 fragment per transcript is generated, FPKM calculations are unnecessary, and a recommended read depth of 2-5 million short, single-end reads per sample is sufficient for expression profiling of eukaryotic transcriptomes. 08:00 Cisbio Help You Make Your Immunoassay a Success: from Research to Drug Development Dr. Ullrike Esslinger Scientific Consultant at Cisbio Bio Assay. Cisbio-Zotal Cisbio Bioassays develops and markets products and technologies used in life sciences and diagnostics. With over 30 years' expertise in assay conception, the company has become the leader in the field of TR-FRET immunoassays with HTRF®, its proprietary technology. Based on this highly robust and reliable format, Cisbio Bioassays offers first class assays and services for drug discovery researchers, from basic research to all steps of new lead identification and optimization. Our kits are already adopted for different researches in CNS, Oncology, Metabolism and Cardiology, but not only. It can also help in your drug development process like HTS or leads optimization. Our scientists are ready to help you to develop custom kit that will perfectly fit your needs. in this session we will share insights about our technology and how it will help you to be successful with your immunoassay. 4 TUESDAY, FEBRUARY 21, 2017 07:30-08:30 EBT3: Early bird session of 3D-Biology – NanoString Technologies Dan hotel - Blue hall A Session participants will be enrolled in a lottery having the chance to win a tablet. 07:30 The Power of 3D-Biology™: Simultaneous Detection of DNA, RNA, and Proteins Rudy van Eijsden, Ph.D. Field Application Specialist, nanoString Technologies The digital NanoString nCounter® technology enables you to count up to 800 molecules per sample in a highly multiplexed fashion. To meet the need for multi-analyte profiling in for example tumor biology, NanoString Technologies has expanded its single-molecule digital barcoding system to recognize DNA, RNA, fusions, proteins and phospho-proteins simultaneously from the same sample with its 3D Biology™ technology. Detection of DNA (e.g. SNVs), RNA, or proteins alone can provide significant information, but when data from multiple experiments are merged the integrated results can lead to novel hypotheses or more solid conclusions. Examples will be presented how 3D Biology™ can add extreme value to your research. Less sample, more data, easy to analyze! TUESDAY, FEBRUARY 21, 2017 08:00-08:30 EBT4: Early bird session of Mercury Dan hotel - Blue hall B Session participants will be enrolled in a lottery having the chance to win a tablet. 08:00 Biomarkers Quantification using Multiplex Technology Improving Result Accuracy and Sensitivity Quantify a full panel of biomarkers is one assay! Dr. Ben Levi Mercury Milliplex® Multiplexing is highly validated and sensitive technique, which enables you to achieve reproducible data while saving precious time. Milliplex® is a lead multiplexing kit, utilizing Luminex 5 technology, employing a simple protocol. Analysis of numerous factors simultaneously made possible within a small volume of biological sample, in a cost-effective manner. TUESDAY, FEBRUARY 21, 2017 09:00-10:45 TUE9: Motors and Machines in Cells Herods hotel - Kings hall A Joint session between Israel Society for Biochemistry and Molecular Biology & Israeli Forum for Cytoskeleton and Motility Chair: Alexander Bershadsky, Weizmann Institute of Science Chair: Leah Gheber, Ben Gurion University 09:00 New Role for the Actin-Based Motor Myosin 19 in Mitochondria Localization Arnon Henn, Boris I. Shneyer, Marko Ušaj Faculty of Biology, Technion - Israel Institute of Technology, Israel 09:20 New Insights into the Role and Regulation of Myosin II in Cytokinesis Shoshana Ravid, Daniel Ronen, Ari Babkoff, Einav Kfir-Cohen, Areen Mghndaf, Adam Gobi Department of Biochemistry and Molecular Biology, The Hebrew University of Jerusalem, Israel 09:40 Microtubule Stability and Motor Proteins Function Can Be Regulated by Neurotrophic Factors Eran Perlson Physiology and Pharmacology, Tel Aviv University, Israel 10:00 Cellular Mechanosensing of Extracellular Matrix Rigidity by Contractile Units Controlled by Receptor Tyrosine Kinases Haguy Wolfenson Department of Genetics and Developmental Biology, Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Israel 10:15 Chlamydomonas Ciliary Tubulin: from Dynamic instability To Length Control Ron Orbach,