See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/237086211 Confirmation of Shared and Divergent Genomes in the Glycine tabacina Polyploid Complex (Leguminosae) Using Histone H3-D Sequences Article in Systematic Botany · July 2000 DOI: 10.2307/2666688 CITATIONS READS 43 32 4 authors, including: Anthony H. D. Brown Bernard E Pfeil CSIRO National Facilities and Collections University of Gothenburg 242 PUBLICATIONS 16,790 CITATIONS 129 PUBLICATIONS 2,471 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: Evolutionary history of Antirrhinum genus View project Phylogeny of Acanthophyllum and allied genera View project All content following this page was uploaded by Anthony H. D. Brown on 09 September 2016. The user has requested enhancement of the downloaded file. Confirmation of Shared and Divergent Genomes in the Glycine tabacina Polyploid Complex (Leguminosae) using Histone H3-D Sequences Author(s): Jeff J. Doyle, Jane L. Doyle, A. H D. Brown, and Bernard E. Pfeil Source: Systematic Botany, 25(3):437-448. Published By: The American Society of Plant Taxonomists DOI: http://dx.doi.org/10.2307/2666688 URL: http://www.bioone.org/doi/full/10.2307/2666688 BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use. Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder. BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research. Systematic Botany (2000), 25(3): pp. 437±448 q Copyright 2000 by the American Society of Plant Taxonomists Con®rmation of Shared and Divergent Genomes in the Glycine tabacina Polyploid Complex (Leguminosae) using Histone H3-D Sequences JEFF J. DOYLE and JANE L. DOYLE L. H. Bailey Hortorium, Cornell University, Ithaca, New York 14853 A. H. D. BROWN and BERNARD E. PFEIL Centre for Plant Biodiversity Research, CSIRO Plant Industry, Canberra ACT2601, Australia Communicating Editor: Alan Whittemore ABSTRACT. Glycine tabacina, a wild perennial relative of soybean, comprises a widespread polyploid com- plex in Australia and islands of the Paci®c Ocean. Data from a single-copy nuclear locus, histone H3-D, con®rm the existence of two polyploid races. Plants of one of these (AAB9B9) are nonstoloniferous and have linear lea¯ets. One of the genomes of this race is that of an A-genome diploid, identi®ed by the histone data most closely with a race of G. tomentella. Its other genome (B9B9) was donated by a nonstoloniferous diploid species that is sister to all of the remaining B-genome species, which are stoloniferous. Plants of the second race of polyploid G. tabacina (BBB9B9) are stoloniferous, have ovate lea¯ets, and combine a B9 genome with a genome of the core B-genome diploid group. The likely source of the shared B9 genome is a species previously referred to as G. sp. aff tabacina, that is here formally named Glycine stenophita. Glycine Willd. subgenus Glycine comprises 16 plastomes (Fig. 1; Doyle et al. 1990b, 1990f). These named diploid (2n 5 40) species native to Austra- data support the hypothesis that the two polyploid lia, as well as several polyploid taxa, some of which forms share only one diploid genome. Lack of have colonized islands of the Paci®c Ocean. One of cpDNA variation among several of the diploid taxa the widespread polyploid groups, the Glycine taba- having A-haplotypes precluded pinpointing the cina complex, is composed of two morphological maternal genome donor of the linear lea¯et (AABB) forms (Costanza and Hymowitz 1987). One of these polyploid. In contrast, of the several chloroplast is stoloniferous with adventitious roots and has haplotypes found among accessions of the ovate ovate to lance-ovate mature lea¯ets, whereas the lea¯et polyploid, six were identical to haplotypes other is nonstoloniferous and has linear mature occurring in the highly polymorphic B-genome dip- lealets. Arti®cial hybrids between the two forms are loid species group (Doyle et al. 1990d). This led to sterile, with meiotic pairing being about half of that the hypothesis that the BBB2B2 polyploid had at found in the usually fertile individuals produced in least six independent origins, presumably involving crosses between different populations of the same different B-genome diploid taxa (Doyle et al. 1990e). form. Based on arti®cial hybridizations between Further evidence as to the contributing genomes various diploid species and each of these polyploid of both types was available from restriction site forms (e.g., Singh et al. 1987, 1992) and on other maps of the biparentally inherited 18S±26S nuclear data (e.g., isozymes: Menacio and Hymowitz 1989), ribosomal gene family (nrDNA). Complex restric- Hymowitz et al. (1998) listed the genome formula tion fragment pro®les suggested that both poly- of the ovate lea¯et form as BBB2B2, and that of the ploid forms were ®xed heterozygotes for nrDNA, linear lea¯et form as AABB. These genome formu- that they shared one genome, and that the donor of lae were intended to signify that the AABB form this genome was a then-unnamed diploid species was an allopolyploid, whereas the BBB2B2 form was of the B-genome group (referred to as G. sp. aff. a partial or segmental allopolyploid, and that the tabacina by Doyle et al. 1990b, 1990d; here formally two forms shared one genome (Fig. 1). named as G. stenophita, see Appendix 1). It was also The two forms also differ consistently throughout hypothesized from these data that the maternal ge- their geographic range in their chloroplast DNA nome donor of the linear lea¯et polyploid was the (cpDNA) haplotypes. Haplotypes of the ovate leaf- D4 diploid isozyme race of G. tomentella, a taxon let (BBB2B2) form belong to the B-plastome group, that has an A-type chloroplast haplotype (Doyle et whereas the linear lea¯et (AABB) form has A-type al. 1990b). Ribosomal gene restriction maps in- 437 438 SYSTEMATIC BOTANY [Volume 25 mowitz et al. (1998) who suggested that G. canescens may be the source of the A-genome and G. micro- phylla or G. latifolia the source of the B- and B2-ge- nomes. Here we address the problem of genome origins in the G. tabacina polyploid complex using DNA se- quences of histone H3-D, a single copy nuclear gene that has been shown to be useful in phylogenetic studies of the genus (Doyle et al. 1996, 1999a, 1999b). MATERIALS AND METHODS Ten accessions of the linear lea¯et (AABB; here termed AAB9B9 for reasons discussed below) poly- ploid were sampled, spanning its geographic range (Table 1). Four accessions of the ovate lea¯et (BBB2B2; henceforth termed BBB9B9) polyploid were FIG. 1. Hypothesis of genomic constitutions and ori- also included in this study. The BBB9B9 polyploid gins of two G. tabacina polyploid types. Relationships was the focus of a companion study (Doyle et al. among diploid (2n 5 38 or 40) Glycine species are sum- 1999b) that used sequences of histone H3-D alleles marized from histone (Doyle et al. 1996 and unpublished from 40 accessions to test the hypothesis of multi- data) and nrDNA ITS (Nickrent and Doyle 1995; Kollipara ple origins of its maternal (B) genome originally et al. 1997; Singh et al. 1998) results. Shown to the right proposed on the basis of cpDNA results (Doyle et of the tree are chloroplast genome groups (Doyle et. al. 9 9 1990a, 1990c, 1990d; the chloroplast genome of G. pindanica al. 1990e). Four of these BBB B accessions were se- has not been studied) and nuclear genome designations lected on the basis of their B-genome histone H3-D (from Kollipara et al. [1997] and Singh et al. [1998], with homoeologue allele and cpDNA haplotype to de- the exception that the G. tomentella D4 isozyme group is termine the sequences of histone alleles at their pu- here assigned the genome symbol A4 to re¯ect its cyto- tative paternal (B9) homoeologous locus. Accessions genetic af®nity with A-genome species). The nuclear ge- representing diploid species for which histone H3- nome of G. stenophita (previously called ``G. sp. aff. G. ta- D sequences had not been obtained previously were bacina'') has not been determined formally but is desig- also included to provide a more complete phylo- nated B9 here; elsewhere it is referred to as B . The mor- 2 genetic context for the study (Table 1). Other se- phologies and hypothesized genome constitutions of the quences used in these phylogenetic analyses were two classes of G. tabacina polyploid are shown at far right, with arrows connecting them to their respective putative reported previously by Doyle et al. (1996, 1999a). nuclear (dashed arrows) and chloroplast (solid arrows) ge- Polymerase chain reaction (PCR) ampli®cation nome donors. and direct cycle sequencing of PCR products were as described in Doyle et al. (1999a). In most cases, ampli®cations were performed with a 59 primer ferred for the ovate lea¯et polyploid were consistent speci®c for histone H3-D (H3D61f) in combination with its second genome being a member of the B- with a general histone H3 39 primer (KV13).