Open Full Page

Open Full Page

CCR FOCUS CCR Focus Targeting the Heterogeneity of Cancer with Individualized Neoepitope Vaccines Ozlem€ Tureci€ 1, Mathias Vormehr2, Mustafa Diken1, Sebastian Kreiter1, Christoph Huber1, and Ugur Sahin1,2,3 Abstract Somatic mutations binding to the patient's MHC and rec- "private" mutations. Concurrence of scientificadvancesand ognized by autologous T cells (neoepitopes) are ideal cancer technological breakthroughs enables the rapid, cost-efficient, vaccine targets. They combine a favorable safety profile due to a and comprehensive mapping of the "mutanome," which is the lack of expression in healthy tissues with a high likelihood of entirety of somatic mutations in an individual tumor, and the immunogenicity, as T cells recognizing neoepitopes are not rational selection of neoepitopes. How to transform tumor shaped by central immune tolerance. Proteins mutated in mutanome data to actionable knowledge for tailoring individ- cancer (neoantigens) shared by patients have been explored ualized vaccines "on demand" has become a novel research as vaccine targets for many years. Shared ("public") mutations, field with paradigm-shifting potential. This review gives an however, are rare, as the vast majority of cancer mutations in a overview with particular focus on the clinical development of given tumor are unique for the individual patient. Recently, the such vaccines. Clin Cancer Res; 22(8); 1885–96. Ó2016 AACR. novel concept of truly individualized cancer vaccination See all articles in this CCR Focus section, "Opportunities and emerged, which exploits the vast source of patient-specific Challenges in Cancer Immunotherapy." Introduction (UTR) of mRNAs, altered proteasomal cleavage sites, or pro- tein-splicing events (7, 8). Genetic abnormalities are key drivers of cancer (1–4). A hun- Neoepitopes are exempt from central tolerance, and thus, dred years ago, Ernest Tyzzer introduced the term "somatic thepresenceofhigh-affinity T cells against them in the mutation" to describe these abnormalities. In a compellingly patient's repertoire is very likely. A considerable number of visionary review (5), he also predicted immune recognition of the neoepitopes accumulating in the course of tumor evolu- somatic mutations and their interindividual variability within tion are recognized by autologous spontaneously occurring tumors of a species, and postulated the existence of tumor-related T cells in mice and in cancer patients and may constitute the immunosuppressive mechanisms (see Box 1). Achilles' heel of tumor cells. In mice, molecular characteriza- Various types of genomic mutations exist: point mutations tion of tumor rejection antigens unraveled mutated epitopes (exchange of one nucleotide by another), insertions/deletions (9–14). Various unique immunodominant mutations were (one or more nucleotides added or removed from the original identified in human cancer patients using tumor-reactive DNA), amplifications/deletions (multiplication or loss of T-cell lines derived from tumor-infiltrating lymphocytes (TIL) copies of a chromosomal region), and translocations and or obtained by repeated stimulation of peripheral blood inversions (interchange of pieces of nonhomologous chromo- T cells with autologous tumor cell lines (refs. 15–17; reviewed somes or reversion of the orientation of a chromosomal in ref. 8). segment; ref. 6). By altering the sequence of translated gene Whereas these data clearly indicate sporadic immune rec- products, mutations may create novel immunogenic epitopes ognition of mutant neoepitopes, the overall relevance of presented on MHC molecules and recognized by T cells mutations for cancer immunotherapy remained controversial (Fig. 1). Neoepitopes may also be created by cancer-associated and ambiguous for a long time. Only recently, a series of alterations in mRNA translation, antigen processing, and pre- independent reports revealed that neoepitope-specificTcells sentation, for example, translation of untranslated regions are crucial for clinical responses mediated by adoptive trans- fer of autologous TILs or by immune checkpoint inhibitors [(refs. 18–23; summarized in refs. 24–27); also see reviews by Hegde and colleagues (28) and Maus and June (29) in 1TRON – Translational Oncology at the University Medical Center of Johannes Gutenberg University, Mainz, Germany. 2Research Center this CCR Focus]. In patients treated with anti–CTLA-4 or anti– for Immunotherapy (FZI), Mainz, Germany. 3Biopharmaceutical New PD-1, the mutational load in the tumor appeared to be an Technologies (BioNTech) Corporation, Mainz, Germany. important predictor of clinical benefit establishing the rele- Corresponding Author: Ugur Sahin, TRON Translational Oncology at the Uni- vance of neoepitopes for rejection of at least melanoma, non– versity Medical Center of Johannes Gutenberg University, Freiligrathstr. 12, smallcelllungcancer(NSCLC),andmismatchrepair–defi- Mainz 55131, Germany. Phone: 4906-1312-1610; Fax: 4906-13121-61100; E-mail: cient colorectal tumors, which are the "signature" cancer types [email protected] for checkpoint blockade. Concordantly, temporary expansion doi: 10.1158/1078-0432.CCR-15-1509 of mutation-specific T cells upon checkpoint blockade was Ó2016 American Association for Cancer Research. reported in some patients and in tumor-bearing mice (20, 30). www.aacrjournals.org 1885 Downloaded from clincancerres.aacrjournals.org on October 2, 2021. © 2016 American Association for Cancer Research. CCR FOCUS human tumor specimen was a potential MHC class I neoepi- Box 1. Relationships between somatic tope (33, 34). mutations and tumor immunity as Various attempts have been made to apply neoantigen- predicted by Tyzzer based vaccine strategies in mouse models. Matsushita and colleagues and Gubin and colleagues showed the feasibility There are marked differences in the behavior of various tumors of using genomic and bioinformatic approaches to identify on transplantation in given classes of mice. Even tumors arising in MHC class I neoepitopes as tumor rejection antigens in a homogenous races show such differences, and this may be attrib- highly immunogenic methylcholanthrene (MCA)-induced ... uted to acquisition of new characteristics, [ ] it appears logical murine sarcoma model (14, 30). In continuation of this work, ... to regard a tumor as a manifestation of somatic mutation[ ]. his laboratory demonstrated in immune-edited variants of the The tissue of the new growth has thus in certain respects become sarcoma model that the antitumor effect of checkpoint block- ... foreign to the other tissues [ ]. Malignant tumors must have ade is mediated by MHC class I neoepitope-specificTcellsand fi feeble antigenic power as well as suf cient resistance to the normal that vaccination with long synthetic peptides encoding these fl inhibiting in uences to provide continued growth in the animal neoepitopes induces the same therapeutic activity as check- fi in which they originate, otherwise reactions suf cient to destroy point inhibition. them would occur more frequently. Our group pioneered the concept of individualized neo- epitope vaccines and proposed a tailored approach to exploit Ernest Edward Tyzzer, "Tumor Immunity," The Journal of the full spectrum of the individual mutations of a patient Cancer Research, 1916 (ref. 5) (14, 30, 34, 35). Vaccination studies with synthetic peptides and mRNA-encoding mutations identified by NGS in three different mouse tumor models revealed that a significant portion of nonsynonymous point mutations (21%–45%) is immunogenic. Unexpectedly,thevastmajorityofthese þ Moreover, recent studies from Laurence Zitvogel's (INSERM, neoepitopes were not recognized by CD8 but were recognized þ U805, Institut Gustave Roussy, 39 Rue Camille Desmoulins, by CD4 T cells. mRNA vaccines encoding single MHC class II F-94805 Villejuif, Paris, France) as well as from Thomas neoepitopes were capable of controlling the growth of estab- Gajewski's (Department of Pathology, University of Chicago, lished mouse B16-F10 melanoma and CT26 colon cancer Chicago, IL) laboratories convincingly demonstrated that tumors. On the basis of these observations, we developed a commensal gut microbes, such as Bacteroides, Burkholderia,or process combining computationalpredictionofrelevantMHC fi Bi dobacterium species, may positively affect the success of class II neoepitopes from NGS exome data with rapid produc- checkpoint blockade treatment in tumor-bearing mice (31, tion of synthetic poly-neoepitope mRNA vaccines. Vaccination 32). Vetizou and colleagues demonstrated that adoptive trans- with such mRNA vaccines induced an inflammatory tumor – þ fer of bacteria-restimulated T cells from anti CTLA-4 anti- microenvironment, mediated induction of a CD8 T-cell – body exposed mice is able to induce tumor growth retarda- response by antigen spread, and resulted in complete rejec- fi tion in tumor-bearing recipient mice (31). This nding indi- tion of established aggressively growing tumors. Interestingly, fi cates that microbial antigen-speci c T cells might be cross- employing the same predictive algorithm on corresponding reactive with neoantigens, thereby supporting antitumoral human cancer types demonstrated an abundance of MHC responses after checkpoint blockade. Snyder and colleagues class II epitopes indicating the applicability of this approach – proposed that anti CTLA-4 checkpoint blockade favors T-cell to human cancers. responses against neoantigens sharing common peptide sub- Duan and colleagues showed that prophylactic vaccination fi string patterns

View Full Text

Details

  • File Type
    pdf
  • Upload Time
    -
  • Content Languages
    English
  • Upload User
    Anonymous/Not logged-in
  • File Pages
    13 Page
  • File Size
    -

Download

Channel Download Status
Express Download Enable

Copyright

We respect the copyrights and intellectual property rights of all users. All uploaded documents are either original works of the uploader or authorized works of the rightful owners.

  • Not to be reproduced or distributed without explicit permission.
  • Not used for commercial purposes outside of approved use cases.
  • Not used to infringe on the rights of the original creators.
  • If you believe any content infringes your copyright, please contact us immediately.

Support

For help with questions, suggestions, or problems, please contact us