NeoBiotechnologies, Inc. 2 Union Square Union City, CA 94587 Tel: 510-376-5603 Email: [email protected] , [email protected] Website: www.NeoBiotechnologies.com Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Recombinant Rabbit Monoclonal Antibody [Clone CALD1/1424R] Catalog No Format Size Price (USD) 800-RBM4-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00 800-RBM4-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 459.00 800-RBM4-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 459.00 Human Entrez Gene ID 800 Immunogen Recombinant human full-length CALD1 protein Human SwissProt Q05682 Host / Ig Isotype Rabbit / IgG Human Unigene 490203 Mol. Weight of Antigen 150kDa Human Gene Symbol CALD1 Cellular Localization Cytoplasmic Human Chromosome 7q33 Species Reactivity Human and Rat. Others not known. Location Positive Control Ovary, Uterus, Blood vessels in all tissues, smooth muscle Synonyms CAD; CALD1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform or leiomyosarcoma 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; CDM; HCAD; LCAD; NAG22 Formalin-fixed, paraffin-embedded human Uterus stained with Caldesmon Rabbit Recombinant Monoclonal Antibody (CALD1/1424R). Specificity & Comments Supplied As Recognizes a protein of 150kDa, which is identified as the high molecular 200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% weight variant of Caldesmon. Two closely related variants of human BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is Storage and Stability predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 has been detected in skeletal muscle. This MAb recognizes only the to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS 150kDa variant (h-caldesmon) in Western blots of human aortic media required. extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in Limitations smooth muscle and non-muscle contraction. This antibody is available for research use only and is not approved for use in diagnosis. Known Applications & Suggested Dilutions Western Blot (1-2ug/ml) Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) Warranty (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM There are no warranties, expressed or implied, which extend beyond this EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes) description. Company is not liable for any personal injury or economic Optimal dilution for a specific application should be determined. loss resulting from this product. Key References 1. Watanabe, K., et al. 2000. Am. J. Clin. Pathol. 113: 663-668. 2. Frid MG, et al. Dev Biol 1992; 153:185.