Proceedings of the 18th Congress of ICVG, Ankara, TURKEY | 7-11 September 2015 Dear Colleagues It will a great pleasure for me to invite you to 18th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG) which will be held in Ankara on 7-11 September, 2015. This will be the first meeting of ICVG in Turkey. Its venue will be Sheraton Hotel in Ankara. We feel honored to host this meeting, and hope that you will enjoy the scientific presentations, networking opportunities, field trips and our beautiful city of Ankara. Hoping to seeing you in Ankara, Prof. Dr. Filiz ERTUNÇ Chair,18th ICVG Organizing Committee Editor: Prof.Dr. Filiz ERTUNÇ Address : Ankara University Faculty of Agriculture, Department of Plant Protection 06110, Ankara / TURKEY Phone Number : +90 312 596 11 20 Fax : +90 312 318 70 29 E-Mail : [email protected], [email protected] Proceedings of the 18th Congress of ICVG, Ankara, TURKEY | 7-11 September 2015 Committees & Invited Speakers ICGV Steering Committee th Giovanni P. Martelli (Italy) 18 ICVG Organizing Committee Mark Fuchs (USA) Filiz Ertunç Johan T. Burger (Republic of South Africa) Özer Elibüyük Fiona Constable (Australia) Didem Canik Orel Nicola Fiore (Chile) Yağmur Türkmen Deborah A. Golino (USA) Meetings Nuredin Habili (Austuralia) 1. Changins (Switzerland) 17-20 August 1964 Raymond Johnson (Canada) 2. Davis (California) 7-11 September 1965 Nikos Katis (Greece) 3. Bernkastel-Kues (W. Germany) September 1967 Olivier Lemaire (France) 4. Colmar (France) 16-18 June 1970 Michael Maixner (Germany) 5. Salice Terme (Italy) 16-19 September 1973 Gustavo Nolasco (Portugal) 6. Cordoba and Madrid (Spain) 12-17 September 1973 Pasquale Saldarelli (Italy) 7. Niagara Falls (Canada) 7-12 September 1980 Edna Tanne (Israel) 8. Bari (Italy) 2-7 September 1984 Honorary Committee Members 9. Kiryat Anavim (Israel) 6-11 September 1987 Guiseppe Belli (Italy) 10. Volos (Greece) 3-7 September 1990 Antoine Caudwell (France) 11. Montreux (Switzerland) 5-10 September 1993 Oscar A. De Sequeira (Portugal) 12. Lisbon (Portugal) 28 September - 2 October 1997 Hans M. Kassemeyer (Germany) 13. Adelaide (South Australia) 12-17 March 2000 Gawrie Kriel (Rebuplic of South Africa) 14. Locorotondo (Italy) 12-17 September 2003 Ali Rezaian (Australia) 15. Stellenbosch (South Africa) 3-7 April 2006 Iannis Rumbos (Greece) 16. Dijon (France) 31 Aug - 4 Sep 2009 Guenther Stellmach (Germany) 17. Davis (California) 7-14 October 2012 Daniel Teliz (Mexico) 18. Ankara (Turkey) 7-11 September 2015 Bernard Walter (France) 18th ICVG Conference Correspondent 18th ICVG Invited Speakers Members Organizing Secretariat : Bilkon Tourism and Travel Agency Assunta Bertaccini Address : Cinnah Cad. Gelibolu Sk. 3/11 Kavaklidere Giovanni P. Martelli 06680 Ankara Turkey Phone Number : +90 312 466 14 66 Fax Number : +90 312 466 14 68 E-Mail : [email protected] Web : www.bilkonturizm.com.tr 4 ICVG 2015 Abstracts Proceedings of the 18th Congress of ICVG, Ankara, TURKEY | 7-11 September 2015 Contents INVITED PRESENTATIONS Viticultural properties and germplasm profiles of Turkey 14 Birhan Marasalı Kunter, Gökhan Söylemezoğlu Grapevine Virology: A historical account with an eye to the studies of the last 60 years or so 16 G.P. Martelli ORAL PRESENTATIONS Session 1: Nepoviruses OP 01 - Tobacco ringspot virus in a wine grape (Vitis vinifera) cultivar in Washington State 24 Rayapati A. Naidu*, Basavaraj Bagewadi, Leslie Walker, Prashant Swamy, Andrew Schultz OP 02 - Determination of the distribution and genetic variation of Grapevine virus A and Grapevine fanleaf 26 virus in vineyards of East and West Azarbaijan provinces Maryam Mikaeili, Mina Rastgou* and Vahid Roumi OP 03 - Hypersensitive-like response to Grapevine fanleaf virus in Nicotiana occidentalis. 28 Emmanuelle Vigne*, Isabelle Martin, Véronique Komar, Marc Fuchs, Olivier Lemaire and Corinne Schmitt-Keichinger. OP 04 - New insights in Nepovirus capsid determinants involved in the transmission by Xiphinema spp 30 nematodes. Lorène Belval, Aurélie Marmonier, Pascale Schellenberger, Peggy Andret-Link, Corinne Keichinger , Sophie Gersch,Véronique Komar, Emmanuelle Vigne, Stefano Trapani, Patrick Bron, Bernard Lorber, Claude Sauter, Marc Fuchs, Olivier Lemaire, Christophe Ritzenthaler* and Gérard Demangeat*. OP 05 - Nanobodies: getting over the hump of fanleaf degeneration? 32 Caroline Hemmer, Léa Ackerer, Aurélie Marmonier, Kamal Hleibieh, Corinne Schmitt-Keichinger, Emmanuelle Vigne, Sophie Gersch, Véronique Komar, Igor Orlov, Bernard Lorber, Lorène Belval, François Berthold, Baptiste Monsion, Bruno Klaholz, Olivier Lemaire, Serge Muyldermans, Gérard Demangeat and Christophe Ritzenthaler*. OP 06 - Grapevine fanleaf virus: towards the viral protein-protein interaction map and host factors 34 identification. François Berthold, Léa Ackerer, Adrien Trolet, Christophe Ritzenthaler, Corinne Schmitt- Keichinger* OP 07 - Findings point to long existence of Grapevine fanleaf virus in Iran 36 Nemat Sokhandan Bashir*, Shaheen Nourinejhad-Zarghani and Mohammad Hajizadeh Session 2: Leafroll and related viruses OP 08 - Grapevine leafroll and red blotch diseases in Washington State vineyards 38 Rayapati A. Naidu*, Bhanupriya Donda, Jati Adiputra OP 09 - Timing of the symptoms influences the management strategies ofGrapevine leafroll-associate 40 virus 3 in New Zealand. Vaughn A. Bell, Kar Mun Chooi, Arnaud G. Blouin , Daniel Cohen, Gerhard Pietersen, Robin M. MacDiarmid OP 10 - Mealybug transmission efficiency of four Grapevine leafroll-associated virus 3 (GLRaV-3) genetic 42 variants A.E.C. Jooste* and Kerstin Krϋger OP 11 - Occurence of Grapevine leafroll-associated viruses (GLRaVs) in Aegean vineyards, Turkey 44 Serkan Önder*, Mustafa Gümüş OP 12 - Grapevine Leafroll associated virus 1 effects on different Grapevine rootstocks 46 Deborah A. Golino, Adib Rowhani*, Vicki Klaassen, Sue T. Sim, Maher Al Rwahnih OP 13 - Detection of Grapevine leafroll-assosiated virus type 3 (GLRaV-3) in Azerbaijan and study of 48 some histopathological changes in leaves of infected plants Irada Huseynova*, Nargiz Sultanova, Alamdar Mammadov, Nargis Kosayeva, Maya Khanishova, Jalal Aliyev ICVG 2015 Abstracts 5 Proceedings of the 18th Congress of ICVG, Ankara, TURKEY | 7-11 September 2015 Session 3: Leafroll and related viruses - II OP 14 - Serological relationships among GLRaV-4 strains reflects the genetic variability of its coat protein 51 gene Sebastian Gomez Talquenca*, Sabrina Moyano and Melisa Lanza Volpe OP 15 - GLRaV-3: diversity, detection and quantitation 53 Hans J Maree *, Rachelle Bester , Michael D Pirie , P Theo Pepler , Kristin Oosthuizen and Johan T Burger OP 16 - Comparative sequence analysis of partial coat protein genes of different Grapevine leafroll- 55 associated virus-1 isolates Eminur Elçi*, Mona Gazel, Kadriye Çağlayan OP 17 - Monitoring in Israel of Grape leafroll disease in new plantings originating from imported plant 57 material. Zahavi Tirtza*, Sharon Rakefet, Sokolsky Tamar, Asor Maor, Crane Omer & Mawasi M. OP 18 - In vitro expression and purification of coat protein gene ofGrapevine leafroll-associated virus 3 59 (GLRaV-3) from grapevine and development of immunodiagnostics for its detection Sandeep Kumar*, Priyanka Singh, Richa Rai and Virendra K Baranwal OP 19 - Occurrence and characterization of Grapevine leafroll-associated virus 4 in Indian Vineyards 61 Richa Rai, Virendra Kumar Baranwal*, S.M.Paul Khurana and Sandeep Kumar Session 4: Rugose wood and related viruses OP 20 - Genetic variability and divergence of Chilean isolates of Grapevine rupestris stem pitting- 64 associated virus Alan Zamorano, Ximena González, Nicola Fiore* OP 21 - Australian Shiraz Disease: Detection of Grapevine Virus A in Shiraz Vines showing a Corky Bark- 66 like Syndrome Nuredin Habili* and Qi Wu Session 5: New viruses, disaeses of unknown etiology and viroids OP 22 - Grapevine leaf mottling and deformation and Grapevine Pinot gris virus: an update on an 68 emerging Mediterranean disease and a new virus Pasquale Saldarelli, Valeria Gualandri, Giovanni Paolo Martelli OP 23 - Identification and determination of full-length sequence of three Grapevine Viroids in a decade 70 old bottled Cabernet Sauvignon Wine Nuredin Habili* Qi Wu, Mohammad Hajizadeh, John W. Randles OP 24 - Is Grapevine red blotch-associated virus the causal agent of red blotch disease? 72 Marc Fuchs*, Björn Krenz, L. Marcela Yepes, Jeremy Thompson, Heather L. McLane and Keith L. Perry OP 25 - Sequence diversity and relationships among Grapevine red blotch virus isolates from vines 74 within and outside a diseased vineyard Keith L. Perry*, Jeremy Thompson, Heather McLane, M. Zeeshan Hyder, and Marc Fuchs OP 26 - Detection and characterization of Chilean isolates of grapevine viroids. 76 Alan Zamorano, Ximena González, Nicolás Quiroga, Nicola Fiore* Session 6: New viruses, disaeses of unknown etiology and viroids - II OP 27 - Studies on Grapevine Pinot gris virus (GPGV) presence and its association with an emergent 78 disease of grapevine Nadia Bertazzon, Vally Forte, Luisa Filippin, Elisa Angelini* OP 28 - Syrah decline in Ontario vineyards 80 Huogen Xiao, Julia Hooker, Caihong Li and Baozhong Meng* OP 29 - The situation of the new emerging grapevine viruses in Turkey 83 Kadriye Çağlayan*, Mona Gazel, Eminur Elçi, Lerzan Öztürk 6 ICVG 2015 Abstracts Proceedings of the 18th Congress of ICVG, Ankara, TURKEY | 7-11 September 2015 OP 30 - Grapevine Pinot gris virus (GPGV) detection in Slovenia 85 Irena Mavrič Pleško*,