BLAST XIII MEETING HILTON TUCSON EAST TUCSON, ARIZONA JANUARY 19-23, 2015 Meeting Chairperson: Dr. Karen Ottemann – University of California - Santa Cruz, Santa Cruz, CA Meeting Vice-Chairperson: Dr. Alan Wolfe – Loyola University, Maywood, IL Program Committee: Dr. Rasika Harshey (Chairperson) – The University of Texas at Austin, Austin, TX Dr. Karen Ottemann – University of California - Santa Cruz, Santa Cruz, CA Dr. Alan Wolfe – Loyola University, Maywood, IL Poster Awards Committee: Dr. Phillip Aldridge – Newcastle University, Newcastle upon Tyne, UK Dr. Rick Dahlquist – University of California at Santa Barbara, Santa Barbara, CA Dr. Jonathan McMurry – Kennesaw State University, Kennesaw, GA Dr. Tarek Msadek – Institut Pasteur, Paris, France Dr. John S. Parkinson – University of Utah, Salt Lake City, UT Dr. Christopher Rao – University of Illinois, Champaign-Urbana, IL Dr. Yuhai Tu – IBM Research, Inc., Yorktown Heights, NY Dr. Laurence Wilson – University of York, York, UK Dr. Kylie Watts (Chairperson) – Loma Linda University, Loma Linda, CA Speaker Award Committee: Dr. Karen Ottemann – University of California - Santa Cruz, Santa Cruz, CA Dr. Alan Wolfe – Loyola University, Maywood, IL Meeting Review Committee: Dr. Penelope Higgs – Wayne State University, Detroit, MI Dr. Jun Liu – University of Texas Houston Medical School, Houston, TX Dr. Birgit Pruess – North Dakota State University, Fargo, ND Dr. Lynmarie Thompson (Chairperson) – University of Massachusetts, Amherst, MA Board of Directors – BLAST, Inc.: Dr. Robert Bourret (Chairperson) – University of North Carolina, Chapel Hill, NC Dr. Joe Falke – University of Colorado, Boulder, CO Dr. Urs Jenal – University of Basel, Basel, Switzerland Dr. Michael Manson – Texas A&M University, College Station, TX Dr. Karen Ottemann – University of California - Santa Cruz, Santa Cruz, CA Conference Coordinators: Ms. Tarra Bollinger – Molecular Biology Consortium, Chicago, IL Ms. Peggy O’Neill – Molecular Biology Consortium, Chicago, IL ii TABLE OF CONTENTS COMMITTEE INFORMATION ........................................................................................ ii TABLE OF CONTENTS ................................................................................................. iii AWARDS INFORMATION .............................................................................................. iv MEETING SCHEDULE ................................................................................................... v SPEAKER PROGRAM ................................................................................................... vi POSTER LIST ................................................................................................................ ix SPEAKER ABSTRACTS ................................................................................................. 1 POSTER ABSTRACTS ................................................................................................. 53 PARTICIPANT LIST .................................................................................................... 113 iii AWARDS INFORMATION Robert M. Macnab Award for an Outstanding Poster Presentation by a Postdoctoral Scientist This award was established at BLAST VIII (2005) and is named in memory of the late Robert M. Macnab, Ph.D., who was an integral member of the Bacterial Locomotion and Signal Transduction Community. Dr. Macnab spent his 30 year career studying the assembly, structure and function of the bacterial flagellum. Bob actively participated in the BLAST meetings and served on the Program and Review Committees for BLAST IV. At the time of his death in 2003, Bob was a professor in the Department of Molecular Biophysics and Biochemistry at Yale University. Robert M. Macnab Memorial Travel Awards We are pleased to announce the establishment of the first travel awards for the BLAST meeting, remembering our colleague Dr. Robert M. Macnab on the 10th anniversary of his death. The intent of the awards is to help young scientists from outside the country that hosts BLAST to attend the meeting. The recipients chosen by the Board of Directors for BLAST XIII are Matthew Baker from Lawrence Lee’s lab at the Victor Chang Cardiac Research Institute, Dinah Tambalo from Chris Yost’s lab at the University of Regina and Yuki Yamanaka from Kaneyoshi Yamamoto’s lab at the Hosei University. (The Macnab poster and travel awards are sponsored by generous donations from Mrs. May K. Macnab) Robert J. Kadner Award for an Outstanding Poster Presentation by a Graduate Student This award was established at BLAST IX (2007) and is named in memory of the late Robert J. Kadner, Ph.D., who was an integral member of the Bacterial Locomotion and Signal Transduction Community. Dr. Kadner spent his career studying microbial physiology of E. coli transport systems. Bob actively participated in the BLAST meetings and served as Chair of the Review Committee for BLAST V, Vice-Chair of BLAST VII and Meeting Chair of BLAST VIII. At the time of his death in 2005, Bob was the Norman J. Knorr Professor of Basic Sciences in the Department of Microbiology at the University of Virginia, School of Medicine. This award is sponsored by BLAST. BLAST Board of Directors' Award for an Outstanding Talk This award was established at BLAST XI (2011) by Phil Matsumura, Founding Chair of the BLAST Board of Directors. The BLAST Board of Directors Award is open to Graduate Students who present a talk at BLAST XIII. BLAST Founders’ Award for an Outstanding Talk The BLAST Board of Directors is pleased to announce the establishment the BLAST Founders’ Award in recognition of those who founded BLAST, Joe Falke, Mike Manson, Phil Matsumura and Sandy Parkinson. The BLAST Founders’ Award is open to Postdoctoral Fellows who present a talk at BLAST XIII. iv BLAST XIII MEETING SCHEDULE TIME EVENT LOCATION Sunday, January 18, 2015 4:00 pm Poster room available for poster setup Gala Rooms A & B 4:00 pm – 7:00 pm Meeting Registration Outer Vistas 7:00 pm – 8:30 pm Dinner Private Dining Room Monday, January 19, 2015 7:30 am – 8:30 am Breakfast Private Dining Room 8:45 am – 9:00 am Welcome & Announcements Salon B & C 9:00 am – 12:00 pm Meeting Session – “Flagella” Salon B & C 10:15 am – 10:30 am Coffee Break Salon A 12:00 pm – 1:30 pm Lunch Private Dining Room 1:30 pm – 4:00 pm Meeting Session – “Flagella Motor” Salon B & C 2:45 pm – 3:00 pm Coffee Break Salon A 4:30 pm – 6:00 pm Postdoc & Student Mixer Private Dining Room 6:00 pm – 7:30 pm Dinner Private Dining Room 7:30 pm – 10:00 pm Poster Session Gala Rooms A & B Poster Groups A and B presented from 7:30 PM – 8:30 PM Poster Groups C and D presented from 8:30 PM – 9:30 PM Tuesday, January 20, 2015 7:30 am – 8:30 am Breakfast Private Dining Room 8:45 am – 12:00 pm Meeting Session – “Chemoreceptors and Signaling” Salon B & C 10:15 am – 10:30 am Coffee Break Salon A 12:00 pm – 1:30 pm Lunch Private Dining Room 1:30 pm – 4:00 pm Meeting Session – “Chemotaxis” Salon B & C 2:45 pm – 3:00 pm Coffee Break Salon A 6:00 pm – 7:30 pm Dinner Private Dining Room 7:30 pm – 10:00 pm Poster Session Gala Rooms A & B Poster Groups A and C presented from 7:30 PM – 8:30 PM Poster Groups B and D presented from 8:30 PM – 9:30 PM Wednesday, January 21, 2015 7:00 am – 8:00 am Breakfast Private Dining Room 8:15 am – 11:30 am Meeting Session – “Motors and Motility” Salon B & C 9:55 am – 10:10 am Coffee Break Salon A 12:00 pm Tours Depart Atrium Lobby 12:15 pm – 1:30 pm Lunch Private Dining Room Thursday, January 22, 2015 7:30 am – 8:30 am Breakfast Private Dining Room 8:45 am – 12:00 pm Meeting Session – “c-di-GMP Signaling/Collective” Salon B & C 10:15 am – 10:30 am Coffee Break Salon A 12:00 pm – 1:30 pm Lunch Private Dining Room 1:30 pm – 4:00 pm Meeting Session – “Two-Component & Other Signaling” Salon B & C 2:45 pm – 3:00 pm Coffee Break Salon A 4:00 pm – 5:00 pm Town Hall Meeting for students & postdocs Salon B & C 6:00 pm – 7:30 pm Dinner Private Dining Room 7:30 pm – 8:15 pm Business Meeting for all attendees Salon B & C 8:15 pm – 8:30 pm Awards Presentation Salon B & C 8:30 pm – 10:30 pm Reception Private Dining Room Friday, January 23, 2015 7:00 am – 8:30 am Breakfast Private Dining Room v BLAST XIII PROGRAM January 19, 2015 Flagella Monday Morning (8:45 am – 12:00 pm) Chair – Seiji Kojima ABSTRACT PRESENTER TITLE PAGE NO. Growth rate control of flagellar abundance differs in E. coli and Philip Aldridge Salmonella enterica sv. Typhimurium 2 A nutrient-tunable bistable switch control motility in Salmonella enterica Santosh Koirala serovar Typhimurium 3 Mutually repressing repressor functions and multi-layered heterogeneity Mary Stewart regulate the bistable Salmonella FliC census 4 Guillaume Paradis Can bacterial filaments regrow? 5 COFFEE BREAK Mutations in B. burgdorferi flagellar export profoundly affect spirochete flagellar assembly, morphology, motility, structure, cell division, and Tao Lin mouse infectivity 6 Marc Erhardt ATPase-independent Type-III protein secretion in Salmonella enterica 7 Molecular emergence: the mechanism for the self-organization of FliG in Matthew Baker the bacterial flagellar motor 8 Structure of H. pylori pseudaminic acid biosynthesis N-acetlytransferase PseH (flagella glycosylation), and its implications for the novel substrate Anna Roujeinikova specificity 9 January 19, 2015 Flagella Motor Monday Afternoon (1:30 pm – 4:00 pm) Chair – Judy Armitage ABSTRACT PRESENTER TITLE PAGE NO. Asymmetric role of conserved charged-residues in rotor-stator interface of Yasuhiro Onoue the