ISRAEL JOURNAL OF ENTOMOLOGY, Vol. 47, pp. 141–158 (30 November 2017) Notes on Nemesia and Iberesia in the J. Murphy spider collection of the Manchester Museum (Araneae: Nemesiidae) SERGEI L. ZON S TEIN The Steinhardt Museum of Natural History, Tel Aviv University, Tel Aviv, 69978 Israel. Email: [email protected] ABSTRACT Representatives of the genera Nemesia Audouin, 1826 and Iberesia Decae & Car doso, 2006 deposited in the John Murphy spider collection of the Manchester Mu seum (University of Manchester, UK) have been re-examined. This part of the collection is found to include ten species of Nemesia and one member of Ibe­ resia. Males of N. santeulalia Decae, 2005 and N. seldeni Decae, 2005, unknown hi­therto,­are­described­for­the­first­time.­Additional­data­on­the­structures­and­ dis tribution of other studied species are also provided. KEYWORDS: Mygalomorphae, Nemesiinae, Mediterranean, taxonomy, trap- door spiders, new records. RESUMEN Los representantes de los géneros Nemesia Audouin, 1826 e Iberesia Decae & Cardoso, 2006 depositados en la colección de arañas de John Murphy del Mu- seo de Manchester (Universidad de Manchester, Reino Unido) han sido re- examinados. Esta parte de la colección incluye diez especies de Nemesia y un miembro de Iberesia. Los machos de N. santeulalia Decae, 2005 y N. seldeni De cae, 2005, desconocidos hasta ahora, se describen por la primera vez. Se pro- porcionan datos adicionales sobre las estructuras y la distribución de otras es- pecies estudiadas. PALABRAS CLAVE: Migalomorphas, nemésidos, región mediterránea, ara ñas tram peras, taxonomía, nuevos registros. INTRODUCTION The specimens, which constitute the object of the current study, represent only a small portion of the copious spider material collected worldwide mainly from mid-1960s until 2001 by an outstanding British arachnologist John A. Murphy. His collection has been recently donated to the Manchester Museum, the University of Manchester, UK. This spider array contains over 75,000 specimens belonging to at least 1,500 species (D. Logunov, pers. comm.). The present study is based on the re-examination of all specimens belonging to the Mediterranean nemesiid genera Nemesia Audouin, 1826 and Iberesia Decae & Cardoso, 2006. Within the studied material, ten species of the former genus and one species of the latter have been found and are listed below along with their col lecting data. In addition, this excercise has resulted in identifying previously un known males of N. santeulalia Decae, 2005 and N. seldeni Decae, 2005, which are­described­herein­for­the­first­time.­ http://www.entomology.org.il/publications; ISSN (online) 2224-6304 urn:lsid:zoobank.org:pub:188C55B7-019C-4DE8-A580-BCBFC41CE24E 142 ISRAEL JOURNAL OF ENTOMOLOGY, VOL. 47, 2017 MateriaLs anD metHODS Specimens from the following collections were studied: MMUM – Manchester Museum, University of Manchester, UK; MNHN – Muséum national d’Histoire naturelle, Paris, France; BMNH – Natural History Museum, London, UK; SMF – Senckenberg Museum, Frankfurt am Main, Germany. Comparative material used in this study: Iberesia Decae­&­Cardoso:­conspecific­males­and­females­of­I. brauni (L. Koch, 1882) from La Palma, Mallorca (BMNH). Figs 1–4: Nemesia ?corsica Simon, AR4328 (1–3) and G7572.3243 (4) males, shown in frontal (1), ventral (2), lateral (3), and dorsal (4) aspects. Scale bars = 2.0 mm. ZONSTEIN: NEMESID SPIDERS IN J. MURPHY COLLECTION 143 Nemesia Audouin: types of N. bristowei Decae, 2005 (MNHN AR14208), N. ibiza Decae, 2005 (MNHN AR14067), N. randa Decae, 2005 (MNHN AR14198), N. seldeni Decae, 2005 (MNHN AR14202), N. santeugenia Decae, 2005 (MNHN AR14197), N. santeulalia Decae, 2005 (MNHN AR14194), N. valenciae Kraus, 1955 (SMF 9432). Dissected copulative organs were macerated in the 10 % potassium hydroxide aqueous solution and exposed for a few minutes in the ethanol solution of Chlora- zol Black. For illustration, the dissected vagina was placed into a small Petri dish filled­with­a­85­%­solution­of­lactic­acid.­Photographs­were­taken­using­a­Zeiss­ Discovery V20 stereomicroscope with a Canon PowerShot G9 camera, and pre- pared using the Helicon Focus 6.3.2 Pro. Measurements were taken using the above stereomicroscope to an accuracy of 0.01 mm, and are given in millimetres. The total body length, as accepted for my- galomorph spiders (Raven & Schwendinger 1995), includes chelicerae but not spinnerets. The diameter of the AME is usually given as the diameter of a sharp- ly­edged­AME­circle­(the­‘pupilʼ).­When­the­AME­cornea­is­well-separated­and­ elevated, and its diameter could be measured, the corresponding data follow in bra ckets. Any eye interdistances counting this parameter are also given in bra- ckets. The length of the sternum was measured along the straight line between the pos terior tip of the sternum and the hindmost part of the labium. Lengths of leg and palp segments were measured on the dorsal side, and lengths of spinneret segments on the ventral side, from the midpoint of the anterior margin to the midpoint of the pos terior margin. The used terms follow Raven (1985): megaspine – an enlarged spine on the male tibia I, metatarsal preening comb – several densely spaced setae forming a crest-shaped structure in the distal part of the metatarsi. The abbreviations used in text are as follows: ALE – anterior lateral eyes, AME – anterior median eyes, d – dorsal, M – megaspine, PLE – posterior lateral eyes, PLS – posterior lateral spinnerets, PME – median lateral eyes, PMS – posterior me dian spinnerets, p – prolateral, pd – prodorsal, pv – proventral, r – retrolateral, rd – retrodorsal, rv – retroventral, v – ventral. TAXONOMY Family Nemesiidae Simon, 1889 Genus Nemesia Audouin, 1826 Nemesia ? corsica Simon, 1914 (Figs 1–8) Nemesia corsica:­Simon­1914:­11,­12,­14,­20,­fig.­27­(♂♀);­Kraus­1955:­372,­pl.­35,­figs­1,­2­(♂);­Le­ Peru­2011:­94,­fig.­64­(♂). Material examined: France:­1♂­(MMUM­G7572.3243),­Corsica­Isle,­Noceta­Co.,­surroundings­of­ Venaco­(42°14'N­09°11'E),­23.v.1989,­J.A.­Murphy.­Originally­identified­as­“Nemesia corsica Simon” (det. J. Murphy, 1990). Distribution: Known only from Corsica Isle, France (WSC 2017). 144 ISRAEL JOURNAL OF ENTOMOLOGY, VOL. 47, 2017 Figs 5–8: Nemesia ?corsica Simon, male G7572.3243: (5) distal segments of pedipalp, showing bulb and embolus, retrolateraly; (6) subapical part of palpal tibia, showing spine comb, and cym bium, dorsal; (7, 8) palpal organ, retrolateral and ventral aspects, respectively. Scale bars for Fig. 5 = 0.50 mm, for Figs 6–8 = 0.25 mm. Notes: The male palp of Nemesia corsica was previously depicted only once (Kraus­1955:­figs­1,­2).­Unfortunately,­those­figures­are­rather­schematic­and­the­ palp of the studied male looks somewhat different (Figs 5–8). ZONSTEIN: NEMESID SPIDERS IN J. MURPHY COLLECTION 145 Figs 9–14: Nemesia spp., spermathecae, dorsal (inside) aspect: (9) Nemesia sp. aff. dubia O. Pickard- Cambridge (G7572.3074); (10, 11) N. ?ibiza Decae (G7572.3262 and G7572.3263, res pec- tively); (12, 13) Nemesia sp. (G7572.3134 and G7572.3264, respectively); (14) N. san teu­ genia Decae (G7572.3071). Scale bars = 0.5 mm. Nemesia sp. aff. dubia O. Pickard-Cambridge, 1874 (Fig. 9) Material examined: France:­1♀­(MMUM­G7572.3074),­Corsica,­Vizzavona,­1000­m,­16.v.1989,­ J.­&­F.­Murphy.­Originally­identified­as­“Nemesia dubia O. Pickard-Cambridge” (det. J. Murphy, 1990). Notes:­The­configuration­of­the­spermathecae­in­the­studied­specimen­(Fig.­9)­ actually matches that illustrated for Nemesia dubia (cf. Blasco Feliu 1986a:­fig.­ 1C). However, the species N. dubia has been hitherto recorded only in the main- land France and adjoining north-eastern Spain (Blasco Feliu 1986b; WSC 2017), and never in Corsica (Helsdingen 2017). Therefore, the presented here as sump- tion concerning the taxonomic position of the studied specimen remains very ten- tative. 146 ISRAEL JOURNAL OF ENTOMOLOGY, VOL. 47, 2017 Nemesia ? ibiza Decae, 2005 (Figs 10, 11) Nemesia ibiza:­Decae­2005:­161,­figs­161–167­(♀);­Le­Peru,­2011:­96,­fig.­73­(♀). Material examined: Spain:­1♀­(MMUM­G7572.3262),­Ibiza­Isle,­San­Carlos,­30­m,­from­burrow­in­ pine­area,­23.ix.1976,­J.­&­F.­Murphy;­1♀­(MMUM­G7572.3263),­Ibiza­Isle,­Puig­de­Perella,­50­m,­ dry­scrubby­hillside,­21.xii.1981,­J.­&­F.­Murphy.­Originally­identified­as­“Nemesia macroce phala Ausserer, 1871” (det. J. Murphy, 1985). Distribution: Known only from Ibiza Isle, Spain (Decae 2005; WSC 2017). Notes: The shape of the spermathecae in both examined females (Figs 10, 11) some- what differs from that in types of N. ibiza­(cf.­Decae­2005:­fig.­67).­Nevertheless,­ these­females­do­not­differ­significantly­from­the­examined­types­of­N. ibiza in a wide array of features, including the habitus, the eye arrangement, the character of the leg spination, and the shape of the spinnerets. It is not thus excluded that the­afore­mentioned­variants­reflect­variability­of­the­spermathecae­in­this­species,­ or may be based on differences in the preparation technique. However, without further study of this question, the current assignment of these specimens to N. ibiza is­only­tentative.­It­should­be­noted­that­the­abovementioned­configuration­of­the­ sper mathecae also resembles that in N. bristowei Decae, 2005 from Mallorca Isle (Decae­2005:­fig.­32).­However,­until­now­none­of­the­same­Nemesia species has been found in both Mallorca and Ibiza (see WSC 2017). Nemesia sp. (Figs 12, 13) Material examined: Greece:­1♀­(MMUM­G7572.3134),­Kefallinia­[=Kefalonia]­Isle,­Pasira,­100­m,­ 21.v.1987,­J.­&­F.­Murphy;­1♀­(MMUM­G7572.3264),­same­island,­Sisia,­100­m,­29.v.1987,­J.­&­F.­ Murphy.­Originally­identified­as­“Nemesia sp (?)” (det. J. Murphy, 1985, 1987). Notes: Judging from a very similar body morphology and especially the almost uni- form­configuration­of­the­vulva,­both­females­from­Greece­may­be­assigned­to­the­ same species. It should be noted that the shape of the vulva in the studied females is distinguishable from that in all geographically closest species of Nemesia, where it is known, i.e.