If you have issues viewing or accessing this file contact us at NCJRS.gov. " "'- u.s. Department of Justice Federal Bureau of Investigation PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON FORENSIC IMMUNOLOGY FBI ACADEMY QUANTICO, VIRGINIA JUNE 23-26, 1986 Proceedings of the International Symposium on Forensic Immunology co 00 L() \0 u, r-l O§ r-l Ql C. If) 05~ Ql 0- 0 ~ o~ Ql E C/) Ql >. c:0 If) C/) ~ * ....,a: () a: z Ql '*02 Ql iii :5 ...... ....,::> a Ql {ij "...:0- Ql c: !!lc: °E ::>;: B 00 {ij c: 0 ~I::> z~ "e CD ~~ :5 .... ~o- S 1::C: Lt .~ Host Laboratory Division Federal Bureau of Investigation June 23-26, 1986 Forensic Science Research and Training Center FBI Academy Quantico, Virginia NOTICE This publication was prepared by the U. S. Government. Neither the U. S. Government nor the U. S. Department of Justice nor any of their employees makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness or usefulness of any information, apparatus, product or process disclosed, or represents that in use would not infringe privately owned rights. Reference herein to any specific commercial product, process or service by trade name, mark, manufacturer or otherwise does not necessarily constitute or imply its endorsement, recommendation or favoring by the U. S. Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the U. S. Government or any agency thereof. Published by: The Laboratory Division Roger T. Castonguay Assistant Director in Charge Federal Bureau of Investigation U. S. Department of Justice Washington, D.C. 20535 International Standard Book Number 0-932115-07-1 Library of Congress Number 87-619829 Printed by the U.S. Government Printing Office Cover: Aerial photograph of the FBI Academy by George February. For sale by the Superintendent of Documents, U.S. Government Printing Office Washington, D.C. 20402 FOREWORD On June 23-26, 1986, the FBI Laboratory hosted an "International Symposium on Forensic Immunology." The symposium was held at the Forensic Science Research and Training Center, Quantico, Virginia, and there were 175 scientists in attendance from industry, university and government laboratories in the United States, Canada, the Federal Republic of Germany, France, Italy, Japan and the United Kingdom. Plenary sessions were held on topics such as the immunologic basis for species differentiation, the biochemical genetics of the ABO and Lewis cellular and soluble antigens, inhibition-elution and mixed agglutination methods, ELISA methodology, quality control of immunology reagents and laboratory safety. In addition, there were short oral and poster presentations and panel discussions on relevant topics. The symposium provided an exchange of ide!lS which, it is hoped, will generate future research and strengthen the scientific merit of the forensic applications of immunology. Consequently, I believe the objectives of this symposium were met by gathering together respected scientists to discuss immunology and forensic applica­ tions. On behalf of the FBI, I would like to thank all those who participated in the symposium. ROGER T. CASTONGUAY Assistant Director-in-Charge FBI Laboratory iii ~----~--~~~~~---.--------------- Program Organizing Committee BARRY L. BROWN JOSEPH J. ERRERA FBI Laboratory }?BI Laboratory F. SAMUEL BAECHTEL JAMES J. KEARNEY FBI Laboratory FBI Laboratory ROBERT SPALDING FBI Laboratory Session Moderators BARRY L. BROWN RANDALL S. MURCH FBI Laboratory FBI Laboratory JOSEPH J. ERRERA MONICA SAENGER FBI Laboratory MOSES SCHANFIELD JAMES J. KEARNEY Allotype Testing Institute FBI Laboratory ROBERT SPALDING ROBERT E. GAENSSLEN FBI Laboratory University of New Haven DAVID J. WERRETT Central Research Establishment v Contents FOREWORD......................................................................................................... iii SECTION I - LECTURES Inhibition, Elution and Mixed Agglutination................................................................... 3 Patrick J. Lincoln Discussion............................................................................................... 12 Immunologic Basis for Species Differentiation............................ ...................................... 15 David C. Benjamin, John C. Herr and Michael P. Woodward Discussion ............................................................................................... 22 The Biochemical Genetics of ABH and Lewis Cellular Antigens.......................................... 23 Rafael Oriol Discussion ............................................................................................... 35 Biochemical Genetics and Methodology for the Forensic Analysis of HLA Antigens.................. 37 Pamela J. Newall Discussion ............................................................................................... 51 The Biochemical Genetics and Methodology for the Analysis of the Gm and Km Antigens........ 53 Graham B. Divall Discussion............................................................................................... 61 The Biochemistry, Genetics and Applications of the ABO and Lewis Soluble Antigens .............. 63 David J. Werrett and B. G. Lang Discussion ............................................................................................... 80 Applications and Implications of ELISAs in Forensic Immunology ....................................... 83 Steven M Fletcher Discussion ............................................................................................... 90 Quality Control of Immunology Reagents....................................................................... 93 Gregory C. Kennedy Safety in the Forensic Immunology Laboratory................................................................ 101 Walter W. Bond Discussion............................................................................................... 108 Perspectives on the Future of Forensic Immunology ...................................... ,.................. 111 George F. Sensabaugh SECTION II - EXTENDED ABSTRACTS Detection of Semen Blood Group Substances by Adsorption-Inhibition, Adsorption-Elution and Enzyme Linked Immunosorbent Assay....................................................................... 119 J. L. Mudd Phenotyping of ABO Subgroups from Cloth Threads........... ............ ................................. 121 J. R. Melvin, Jr., T. L. Wolson, D. L. Keagle and L. R. Simson Two-Dimensional Adsorption-Inhibition.......................................................................... 125 H. C. Lee, R. E. Gaensslen, E. M Pagliaro and B. Novitch ABH Typing of Urine by a Two-Dimensional Adsorption-Inhibition Procedure... ..................... 127 H. C. Lee, R. E. Gaensslen and E. M Pagliaro ABO Typing of Dried Bloodstains Mixed With Body Fluids by Methanol Extraction................ 129 M A. Tahir and D. J. Czech Separation of Blood Group Substances ABH and Lewis in Mixtures of Body Fluids................. 131 S. L. Bolton and J. W. Thorpe ABH Blood Group Substance Levels in Matched Body Fluid Stains: A Preliminary Investigation....................................................................................................... 135 T. F. Spear and S. A. Binkley vii ABH and Lewis Typing: Application to f ,lected Problems in Mixtures of Semen and Vaginal Secretions. 137 M. M Gibbons, E. T. Blake and G. F. Sensabaugh Levels and Distribution of ABH and Lewis Blood Group Substances in Vaginal Secretions......... 139 M M Gibbons, E. T. Blake and G. F. Sensabaugh Interpretation of Serologic Results................................................................................ 141 H. C. Lee and R. E. Gaensslen Application of the Immunohistochemical Technique to the ABO Blood Grouping of Human Head Hair......................................................................................................... 145 S. Miyasaka and S. Seta Use of an Enzyme Linked Immunosorbent Assay for the Detection of P30 in Questioned Semen Stains...................................................................................................... 147 F. S. Baechtel, J. C. Jung and L. D. Terrell Purification of Seminal Vesicle Specific Antigen by Immunoaffinity Chromatography on Bound Monoclonal Antibody MHS-5 .................................................................................. 149 J. C. Herr, R. S. McGee and D. J. Conklin ELISA Assay for Human Semen Identification Using a Monoclonal Antibody to a Seminal Vesicle Specific Antigen......................................................................................... 153 J. C. Herr and M P. Woodward Enzyme Immunoassay for M Antigen in Bloodstains................................. .............. .......... 157 G. F. Sensabaugh, R. J. Kochenburger and L. M Calandro Determination of ABO Blood Groups from Human Bloodstains and Body Fluid Stains with Mouse Monoclonal Antibodies................................................................................. 159 H. Mukoyama and 1. Sakai The Forensic Application of Anti-A and Anti-B (Murine Monoclonal Blend) Bioclone™ Antisera to the Examination of Blood and Body Fluids in the Crime Laboratory............................. 161 T. L. Wolson and J. R. Melvin, Jr. Use of Monoclonal Antibodies Against ABO(H) Antigens: Detection of Soluble Blood Group Substances in Semen............................................................................................