Calcium Detection Probes & Assay Kits Fluo-8® • Cal-520® • Calbryte™ 520 SIE HABEN DIE VISION, WIR HABEN DIE SUBSTANZ. Table of Contents General Information ............................................................................................................................................................................................ 2 Non-Fluorescent Calcium Signaling Molecules & Chelators ............................................................................................................................ 5 NAADP ............................................................................................................................................................................................................................................................... 5 NAADP-AM .......................................................................................................................................................................................................................................................5 Fluorimetric cADP-Ribose Assay ................................................................................................................................................................................................................. 6 BAPTA AM .......................................................................................................................................................................................................................................................... 6 EGTA AM ............................................................................................................................................................................................................................................................ 6 Pluronic® F-127 ................................................................................................................................................................................................................................................7 Probenecid ........................................................................................................................................................................................................................................................ 7 Fluorescent Single Wavelength Calcium Indicators ..........................................................................................................................................8 Blue-Green Fluorescent Calcium Indicators ...............................................................................................................................................................................................9 Cal-500™ .......................................................................................................................................................................................................................................................9 Green Fluorescent Calcium Indicators .......................................................................................................................................................................................................... 10 Traditional Green Fluorescent Calcium Indicators ..............................................................................................................................................................................10 Fluo-8® .............................................................................................................................................................................................................................................................10 Cal-520® ...........................................................................................................................................................................................................................................................11 CalbryteTM 520 ................................................................................................................................................................................................................................................12 Red Fluorescent Calcium Indicators .............................................................................................................................................................................................................. 13 Cal-590TM ..........................................................................................................................................................................................................................................................13 CalbryteTM 590 ................................................................................................................................................................................................................................................14 Cal-630TM ..........................................................................................................................................................................................................................................................14 CalbryteTM 630 ................................................................................................................................................................................................................................................15 Rhod-4TM ..........................................................................................................................................................................................................................................................16 NIR Fluorescent Calcium Indicators ................................................................................................................................................................................................................17 Cal-670TM ..........................................................................................................................................................................................................................................................17 Cal-770TM .........................................................................................................................................................................................................................................................17 Fluorescent Ratiometric Calcium Indicators ....................................................................................................................................................18 BTC ....................................................................................................................................................................................................................................................................18 Fura-2 .............................................................................................................................................................................................................................................................. 18 Fura-8TM .......................................................................................................................................................................................................................................................... 18 Indo-1 ............................................................................................................................................................................................................................................................. 18 Cal RedTM R525/650 ......................................................................................................................................................................................................................................19 Luminescent Calcium Indicators .......................................................................................................................................................................20 Live Cell Calcium Assays ....................................................................................................................................................................................21 FLIPR® Calcium Assays ................................................................................................................................................................................................................................21 Endpoint Calcium Assay ........................................................................................................................................................................................................................... 23 Flow Cytometric Calcium Assay .............................................................................................................................................................................................................. 23 GPCR Cell Line Calcium Assays ................................................................................................................................................................................................................ 24 cAMP & Phosphodiesterase (PDE) Assays ......................................................................................................................................................