Engineering Application of Nanoscale Biology Special topic: AGE498-KB Spring 2006 University of Illinois at Urbana-Champaign Kaustubh D. Bhalerao, Ph.D. 1304 W Pennsylvania Ave #376A Urbana, IL 61801 [email protected], (217)244-6569 My training • 1999 B.S. Civil Engineering, University of Pune, India • 2001 M.S. Department of Agricultural and Biological Engineering, The Ohio State University, Columbus, Ohio • 2004 Ph.D. Department of Agricultural and Biological Engineering, The Ohio State University, Columbus, Ohio What is this course about? • Engineering • Nanotechnology • Condensed matter (Q-dots, nanotubes) • Biological (proteins, nucleic acids) • Extensions to Biotechnology Format of the course • Two lectures per week • One discussion session per week • One midterm, one final - each with an in- class component and a take-home component • One proposal that will evolve over the duration of the semester • Details in course outline handout Texts for the course • Bhushan B., 2004 Springer Handbook of Nanotechnology. Springer • Goodsell D., 2004 Bionanotechnology: Lessons from Nature. Wiley-Liss. • Niemeyer C., 2004 Nanobiotechnology: Concepts, Applications and Perspectives. John Wiley & Sons • ...no they are not required. Course organization • Progressing from: • What you can do...(components, processes) to • How you may be able to do it. (techniques) • Interwoven with: • Why you should (or shouldn’t) do so. Course objectives • Understand what is (bio)nanotechnology • Learn molecular technologies from a “systems” perspective • Learn how to analyze and design nanodevices and nanosystems • Appreciate the implications (bio) nanotechnology may have on our world. Exam structure • Two exams: midterm and final • Each exam has two sections: • In-class multiple choice closed book, closed notes, time-bound quiz and • Take home written exam with 5-8 questions requiring critical analytical thinking. Semester Design Project • DARPA-style proposal for a biological nanodevice / nanosystem with the following components, each roughly every 4 weeks. • Abstract of idea • Quad-chart • 5 page proposal • 15 minute presentation Discussion series • Discussion of journal articles, news articles and other materials related to (biological) nanotechnology / biotechnology etc. • Class participation will contribute to the overall grade University of Illinois at Urbana-Champaign Engineering Application of Nanoscale Biology Agricultural Engineering AGE498-KB Grade components Grading: Component Points In-class Exam (2@50 points/exam) 100 Take-home Exam (2@25 points/exam) 50 Proposal Outline 5 Quad-Chart 15 5-page proposal 20 15-minute Presentation 15 Class participation and discussion 20 Total 225 Approximate Grading Scale: Above 90% A 80%-90% B 70%-80% C 60%-70% D Below 60% F Page 2 of 2 Course topics 1. Introduction / What is nanotechnology? 2. Synthetic Nanostructures: Carbon Structures / Quantum Dots / Polymers 3. Top-down and Bottom-up manufacturing 4. Molecular Biology Overview 5. Biologic Nanostructures: DNA / Proteins... Course topics 6. UML: A Framework for Collaboration 7. Targeting and Triggering Systems in Nature 8. Power Generation and Control in Nature 9. Nano-Device Design: Rationale, Examples 10. Nanopores and Molecular Filtration 11. Applications: Sensors, Drug Delivery Course topics 12. Complexity in the Nano-World (??) 13. Pharmakokinetics, Immune System 14. Biomimetic Strategies 15. Techniques in Biotech: Molecular Cloning, Genetic Engineering, Antibody production etc. 16. Tools in Bio-nano Research: Non-biological and Biological. Questions about the course organization? What is your idea of nanotechnology? What is your idea of nanotechnology? • Materials / devices / systems sized between 1 and 100 nm (1 nm = 10e-09 m) • Precise atom-by-atom or molecule-by- molecule manipulation to synthesize new devices • Systems which accrue functionality due to features that happen to be nanometer sized Nanotechnology • Nanometer-sized materials / devices / systems a) nacre in red-abalone b) synthetic ZnO crystals c) a diatom d) to h) different types of synthetic silica crystals. Image credit: Sandia National Labs. http://www.sandia.gov/news-center/news-releases/ 2003/mat-chem/nanocrystals.html Nanotechnology Atomically precise manufacturingTherapeutic Nanodevices 10.3 Technological and Biological Opportunities 289 • Self-Assembly of Nanostructures a) Self-assembly has been long recognized as a poten- tially critical labor-saving approach to construction of nanostructures [10.40], and many organic and in- organic materials have self-assembly properties that can be exploited to build structures with controlled configurations. Self-assembly processes are driven by P a thermodynamic forces and generally result in struc- r t tures that are not covalently linked. Intra/intermolecular A forces driving assembly can be electrostatic or hy- 1 0 drophobic interactions, hydrogen bonds, and van der . b) Waals interactions between and within subunits of the 3 self-assembling structures and the assembly environ- ment. Thus, final configurations are limited by the ability to “tune” the properties of the subunits and control the assembly environment to generate particular structures. Self-Assembly of Carbon Nanostructures Carbon nanotubes (Fig. 10.7) spontaneously assemble into higher order [10.31] structures (nanoropes) as c) the result of hydrophobic interactions between indi- vidual tubes. Multi-wall carbon nanotubes (MWCNT) are well-known structures that can be viewed as self- assembled, nested structures of nanotubes with tube diameters decreasing serially from the outermost to in- nermost tubes. The striking resemblance that MWCNT have to macroscale bearings has been noted and ex- ploited [10.41]. MWCNT linear bearings can be actuated by application of mechanical force to the inner nano- tubes of the MWCNT assembly. Actuation causes the assembly to undergo a reversible telescoping motion. Interestingly, these linear MWCNT bearings exhibit es- Fe on Cu sentially no wear as the result of friction between bearing components. Images from the IBM Image Gallery C60 fullerenes and single wall carbon nanotubes Fig. 10.6 (a) A schematic depiction of an atomic force mi- (SWCNT) also spontaneously assemble (Fig. 10.7) into croscope cantilever and tip interacting with materials on higher order nanostructures called “peapods” [10.42] in a surface. Tips typically have points of 50 nm or less in which fullerene molecules are encapsulated in nano- diameter [10.31, 39]. (b) Schematic of multiplexed AFM tubes. The fullerenes of peapods modulate the local tips performing multiple operations in parallel [10.31, 39]. electronic properties of the SWCNT in which they are (c) AFM image of a quantum corral, a structure built us- encapsulated and may allow tuning of carbon nano- ing AFM manipulation of individual atoms (from the IBM tube electrical properties. The potentially fine-level Image Gallery) control of nanotube properties may prove useful in nanotube-containing electrical devices, particularly in cases wherein nanotubes are serving as molecular wires. systems, or in massively parallel industrial microfab- In this capacity, carbon nanotubes have been incorpo- rication approaches. In any case, therapy for a single rated into FETs (field effect transistors, discussed below patient may involve billions of billions of individual nan- under sensing architectures [10.43]), and other molecu- otherapeutic units, so each individual nanotherapeutic lar electronic structures. Ultimately, these architectures structure must require only minimal input from a human may result in powerful, ultra-small computers to provide synthesis/manufacturing technician. the intelligence of “smart,” indwelling nanotherapeutic Springer Handbook of Nanotechnology 1 B. Bhushan • ! Springer 2004 Nanotechnology • Systems with functionality due to specific nanoscale features. Molecular Motor animations: http://nature.berkeley.edu/~hongwang/Project/ATP_synthase/ Image credits: Nanotubes: http://www.tipmagazine.com/tip/INPHFA/vol-10/iss-1/images/24-1.gif F1ATPase Biological Nanotechnology • Biology provides the functional components • Materials science teaches how to synthesize them into systems • Together, we have the ability to create systems unknown in nature Image source unknown roughness was calculated at 13.7 nm. By comparison, zein adsorbed to the hydrophobic surface appears featureless (Figure 6) with higher uniformity and a lower calculated roughness of 2.2 nm. Zein tubular structures may find roughness was calculated at 13.7 nm. By comparison, zein adsorbed to the application in delivery systems for bioactive components. hydrophobic surface appears featureless (Figure 6) with higher uniformity and a lowerZein-based calculated nanoporous roughness structure of 2.2 nm. as aZein biomaterial tubular structures may find applicationFrom AFM inexperiments, delivery systems it is seen for that bioactive zein can components. form tubules having a diameter Zein-basedfrom 100nm nanoporous to 400nm on structure carboxylic as surfaces. a biomaterial Such tubules could be assembled with controlled precision and higher order structures can be designed to serve as From AFM experiments, it is seen that zein can form tubules having a diameter carriers to encapsulate bioactive compounds. Bioactive compounds are from 100nm to 400nm on carboxylic surfaces. Such tubules could be assembled extranutritional constituents that typically occur in small quantities in foods. with controlled precision and higher order structures can be designed