Development of methods to monitor and control Aphanomyces root rot and black root rot of beans. Andrew Watson NSW Department of Primary Industries Project Number: VG08043 VG08043 This report is published by Horticulture Australia Ltd to pass on information concerning horticultural research and development undertaken for the vegetables industry. The research contained in this report was funded by Horticulture Australia Ltd with the financial support of the vegetables industry. All expressions of opinion are not to be regarded as expressing the opinion of Horticulture Australia Ltd or any authority of the Australian Government. The Company and the Australian Government accept no responsibility for any of the opinions or the accuracy of the information contained in this report and readers should rely upon their own enquiries in making decisions concerning their own interests. ISBN 0 7341 3057 0 Published and distributed by: Horticulture Australia Ltd Level 7 179 Elizabeth Street Sydney NSW 2000 Telephone: (02) 8295 2300 Fax: (02) 8295 2399 © Copyright 2013 VG08043 (July 2012) Development of methods to monitor and control Aphanomyces root rot and black root rot of beans. Andrew Watson et al. NSW DPI VG080043 Andrew Watson NSW Department of Primary Industries Yanco Agricultural Institute Yanco, 2703. Phone 02 69 512611 Fax 02 69 512719 Email [email protected] Co-authors: Dr Hoong Pung (Peracto-Tasmania) and Dr Alan McKay (SARDI) Researchers in New South Wales-Andrew Watson, Lee Browne, Meryl Snudden and Liz Mudford Researchers in Tasmania-Hoong Pung and Susan Cross Researchers in South Australia- Alan McKay, Herdina, Ina Dumitrescu, Russell Burns, Danuta Pounsett, Irena Dadej, Aidan Thomas(SARDI) and Di Hartley (CSIRO). This report covers the activities undertaken during the period of the project from January 2009 till July 2012 examining methods to detect and control soil borne diseases of beans. Report Completed–July 2012. This project has been funded by HAL using the vegetable levy and matched funds from the Australian Government. Any recommendations contained in this publication do not necessarily represent current HAL Limited policy. No person should act on the basis of the contents of this publication, whether as to matters of fact or opinion or other content, without first obtaining specific, independent professional advice in respect of the matters set out in this publication. CONTENTS CONTENTS.............................................................................................................................. 1 MEDIA SUMMARY................................................................................................................ 4 TECHNICAL SUMMARY ..................................................................................................... 5 INTRODUCTION.................................................................................................................... 7 Diseases of green beans.............................................................................................................. 7 Aphanomyces root rot ................................................................................................................ 9 Fungicidal control .................................................................................................................... 10 NEW SOUTH WALES RESEARCH ACTVITIES................................................................................... 12 1. The effect of soil solarisation on A. euteiches survival.................................................... 12 Introduction .............................................................................................................................. 12 Method ..................................................................................................................................... 12 Method of disease assessment.................................................................................................. 12 Wetting up period..................................................................................................................... 13 Statistical analysis .................................................................................................................... 14 Results ...................................................................................................................................... 14 Discussion ................................................................................................................................ 16 2. The effect of A. euteiches on yield of green beans. .......................................................... 18 Introduction .............................................................................................................................. 18 Method ..................................................................................................................................... 18 Results ...................................................................................................................................... 19 Discussion ................................................................................................................................ 21 3. The incidence of Aphanomyces root rot in Tasmanian and Victorian bean growing soils. 23 Introduction .............................................................................................................................. 23 Method ..................................................................................................................................... 23 Results ...................................................................................................................................... 24 Discussion ................................................................................................................................ 28 4. DNA soil test for Aphanomyces root rot.......................................................................... 29 Introduction .............................................................................................................................. 29 Method ..................................................................................................................................... 29 Results ...................................................................................................................................... 30 Discussion ................................................................................................................................ 31 5. Biofumigation Trials.......................................................................................................... 32 Introduction .............................................................................................................................. 32 Method ..................................................................................................................................... 32 Results ...................................................................................................................................... 33 Discussion ................................................................................................................................ 34 6. Rotation Trial..................................................................................................................... 35 Introduction .............................................................................................................................. 35 Method ..................................................................................................................................... 35 Results ...................................................................................................................................... 37 Discussion ................................................................................................................................ 40 7. Lime application to reduce Aphanomyces root rot......................................................... 42 Introduction .............................................................................................................................. 42 Method ..................................................................................................................................... 42 Results ...................................................................................................................................... 42 Discussion ................................................................................................................................ 42 8. Greenhouse trials to control Aphanomyces root rot....................................................... 43 1 Introduction .............................................................................................................................. 43 Results ...................................................................................................................................... 44 Discussion. ............................................................................................................................... 46 9. Using antagonistic bacteria to control aphanomyces...................................................... 48 Introduction .............................................................................................................................. 48 Method ....................................................................................................................................