SAN TA C RUZ BI OTEC HNOL OG Y, INC . ZNF37A (ZNF3A11A9): sc-81141 BACKGROUND PRODUCT Zinc-finger proteins contain DNA-binding domains and have a wide variety of Each vial contains 100 µg IgG 1 in 1.0 ml of PBS with < 0.1% sodium azide functions, most of which encompass some form of transcriptional activation and 0.1% BSA. or repression. The majority of zinc-finger proteins contain a Kruppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, APPLICATIONS thereby recruiting histone modifying proteins. ZNF37A, also called KOX21, is ZNF37A (ZNF3A11A9) is recommended for detection of ZNF37A of human a member of the Kruppel C 2H2-type zinc-finger family of transcriptional regu - origin by Western Blotting (starting dilution 1:200, dilution range 1:100- lators. Located in the nucleus, ZNF37A is a 561 amino acid protein containing 1:1000) and immunoprecipitation [1–2 µg per 100–500 µg of total protein (1 one KRAB domain and 12 C 2H2-type zinc fingers. The gene encoding ZNF37A ml of cell lysate)]. is found in a KOX zinc-finger cluster located on chromosome 10. Suitable for use as control antibody for ZNF37A siRNA (h): sc-90767, ZNF37A REFERENCES shRNA Plasmid (h): sc-90767-SH and ZNF37A shRNA (h) Lentiviral Particles: sc-90767-V. 1. Tunnacliffe, A., Liu, L., Moore, J.K., Leversha, M.A., Jackson, M.S., Papi, L., Ferguson-Smith, M.A., Thiesen, H.J. and Ponder, B.A. 1993. Duplicated Molecular Weight of ZNF37A: 65 kDa. KOX zinc finger gene clusters flank the centromere of human chromosome 10: evidence for a pericentric inversion during primate evolution. Nucleic RECOMMENDED SECONDARY REAGENTS Acids Res. 21: 1409-1417. To ensure optimal results, the following support (secondary) reagents are 2. Becker, K.G., Nagle, J.W., Canning, R.D., Biddison, W.E., Ozato, K. and recommended: 1) Western Blotting: use goat anti-mouse IgG-HRP: sc-2005 (dilution range: 1:2000-1:32,000) or Cruz Marker™ compatible goat anti- Drew, P.D. 1995. Rapid isolation and characterization of 118 novel C 2H2-type zinc finger cDNAs expressed in human brain. Hum. Mol. Genet. 4: 685-691. mouse IgG-HRP: sc-2031 (dilution range: 1:2000-1:5000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 3. Jackson, M.S., See, C.G., Mulligan, L.M. and Lauffart, B.F. 1997. A 9.75- sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- Mb map across the centromere of human chromosome 10. Genomics 33: itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). 258-270. 4. Jackson, M.S., Rocchi, M., Thompson, G., Hearn, T., Crosier, M., Guy, J., DATA Kirk, D., Mulligan, L., Ricco, A., Piccininni, S., Marzella, R., Viggiano, L. and 100 K – Archidiacono, N. 1999. Sequences flanking the centromere of human chro - 80 K – mosome 10 are a complex patchwork of arm-specific sequences, stable 60 K – duplications and unstable sequences with homologies to telomeric and 50 K – other centromeric locations. Hum. Mol. Genet. 8: 205-215. 40 K – < ZNF37A fusion 5. Guy, J., Hearn, T., Crosier, M., Mudge, J., Viggiano, L., Koczan, D., Thiesen, 30 K – protein H.J., Bailey, J.A., Horvath, J.E., Eichler, E.E., Earthrowl, M.E., Deloukas, 20 K – P., French, L., Rogers, J., Bentley, D. and Jackson, M.S. 2003. Genomic sequence and transcriptional profile of the boundary between pericen - ZNF37A (ZNF3A11A9): sc-81141. Western Blot analysis tromeric satellites and genes on human chromosome arm 10p. Genome of human recombinant ZNF37A fusion protein. Res. 13: 159-172. RESEARCH USE 6. O’Geen, H., Squazzo, S.L., Iyengar, S., Blahnik, K., Rinn, J.L., Chang, H.Y., Green, R. and Farnham, P.J. 2007. Genome-wide analysis of KAP1 binding For research use only, not for use in diagnostic procedures. suggests autoregulation of KRAB-ZNFs. PLoS Genet. 3: e89. PROTOCOLS CHROMOSOMAL LOCATION See our web site at www.scbt.com or our catalog for detailed protocols and Genetic locus: ZNF37A (human) mapping to 10p11.21. support products. SOURCE ZNF37A (ZNF3A11A9) is a mouse monoclonal antibody raised against a recombinant protein corresponding to a region near the N-terminus of ZNF37A of human origin. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.