ELA2 monoclonal antibody, clone Gene Symbol: ELA2 AOCI-5 Gene Alias: GE, HLE, HNE, NE, PMN-E Catalog Number: MAB20237 Gene Summary: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to Regulatory Status: For research use only (RUO) elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene Product Description: Rabbit monoclonal antibody hydrolyzes proteins within specialized neutrophil raised against synthetic peptide of human ELA2. lysosomes, called azurophil granules, as well as proteins Clone Name: AOCI-5 of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role Immunogen: A synthetic peptide corresponding to in degenerative and inflammatory diseases by its human ELA2. proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane Host: Rabbit protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Reactivity: Human Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia Applications: Flow Cyt, ICC, IF, IHC-P, WB-Ce (SCN). This gene is clustered with other serine protease (See our web site product page for detailed applications gene family members, azurocidin 1 and proteinase 3 information) genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are Protocols: See our web site at packaged together into azurophil granules during http://www.abnova.com/support/protocols.asp or product neutrophil differentiation. [provided by RefSeq] page for detailed protocols Form: Liquid Purification: Affinity purification Isotype: IgG Recommend Usage: Flow Cytometry (1:30) Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunohistochemistry (1:100-1:500) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. Storage Buffer: In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage Instruction: Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. Entrez GeneID: 1991 Page 1/1 Powered by TCPDF (www.tcpdf.org).