Cambridge Isotope Laboratories, Inc. Stable Isotopes for Mass Spectrometry Proteomics Metabolism/Metabolomics MS/MS Standards Environmental Analysis Enriching Scientific Discovery MS_CATALOG 4/14 Supersedes all previously published literature isotope.com Welcome Welcome Over the past decade, there have been vast improvements in the detection and quantification of proteins, metabolites and potential biomarkers using mass spectrometry-based methodologies. Advances in bioinformatics and instrumentation, combined with the use of stable isotopes, have furthered the development, sensitivity and accuracy of quantitative methods. It is with great pride that we present Cambridge Isotope Laboratories, Inc.’s (CIL) new “Stable Isotopes for Mass Spectrometry” catalog. In this catalog, you will find a comprehensive listing of our isotope-enriched products that can be utilized for a wide range of mass spectrometry-based fields of research, including proteomics, metabolism, metabolomics, clinical diagnostics and environmental analysis. Complementing the extensive product listing are contributions written from some of the world’s most prominent mass spectrometrists, biochemists and research scientists. We are delighted to showcase application notes, customer perspectives and testimonials from many leaders in the field, illustrating the utility of stable isotopes. We would like to thank and show our appreciation to the many researchers who have contributed to this catalog. Please see page 163 for a complete list of contributors. CIL continues to maintain a leadership role in developing new products to study proteins, protein turnover, metabolic disorders and environmental contaminants. It has been through our partnerships and close relationships with our customers over the past 30 years that we have been able to significantly expand our product offering in order to assist this community in the advancement of their studies utilizing stable isotope-labeled compounds as a tool in mass spectrometry. We welcome suggestions from our customers for new products, which will advance their research. Our business is stable isotopes, but our focus is you, the customer. Thank you for giving us the opportunity to partner with you since 1981. You have truly made our success possible. Tasha Agreste Proteomics Product Manager Krista Backiel Metabolic Product Manager Carol Beland MS / MS Standards Product Manager Terry Grim Environmental Product Manager Tasha Agreste, Carol Beland, Terry Grim and Krista Backiel Cover illustration by Pedro Lalli www.isotope.com | [email protected] 1 Cambridge Isotope Laboratories, Inc. 2 tel: +1-978-749-8000 | 800-322-1174 (USA) | fax: +1-978-749-2768 isotope.com Corporate Overview Corporate Overview Cambridge Isotope Laboratories, Inc. (CIL) is the world leader in the separation and manufacture of stable isotopes and stable isotope-labeled compounds. With over 400 employees and laboratories in four countries, CIL specializes in the process of labeling biochemical and organic compounds with highly enriched, stable (non-radioactive) isotopes of carbon, hydrogen, nitrogen and oxygen. Our chemists substitute a common atom for a rare, highly valued isotopic component so that the final product can be readily measured or traced using mass spectrometry (mass spec) or nuclear magnetic resonance (NMR). CIL’s products are utilized in laboratories, medical, government and academic research centers and health care facilities worldwide. We are proud that CIL products have contributed to medical advancements in cancer research, new drug development, environmental analysis, genomics and proteomics and medical diagnostic research. In the past decade, as the fields of proteomics and metabolomics CIL’s state-of-the-art production facilities are located at the have developed as leading techniques for determining bio­­­- company’s headquarters in Tewksbury, Massachusetts, and the markers for disease presence, progression and the monitoring company’s primary production laboratories are in Andover, of therapeutic response, CIL has worked closely with industry Massachusetts. Our isotope-separation facility located in Xenia, leaders and researchers to provide the stable isotope-labeled Ohio, houses the world’s largest 13C and 18O isotope-separation tools needed for improved quantitation of complex systems. facilities and the world’s only commercial D2O enrichment columns. CIL’s vision began when it was founded in 1981 by Dr. Joel Bradley, an organic chemist from MIT. Drawing on a commitment to high Dr. Bradley and the CIL Executive Team all share the same quality products, superior customer service, innovative new commitment to quality and service. Our experts collaborate products and breadth of product lines, CIL quickly emerged as a with all of our customers to aid in pivotal research that is being leader in its field. CIL now produces more than 15,000 products conducted in laboratories worldwide. Our partnerships not only and has ISO 13485 quality systems, as well as cGMP production help to support our global reach, but allow us to bring forward capabilities. Over the past several decades CIL has increased its innovative products to aid our customers’ pursuit of scientific global footprint and now has over 400 employees in its facilities discovery. located in four countries. The CIL group is comprised of six companies: Cambridge Isotope Laboratories, Inc. (CIL), CIL Isotope Separations (CIS) and Membrane Receptor Technologies (MRT) in the US; CIL Canada, Inc. in Montreal, Canada; Euriso-Top in Saclay, France; and ABX GmbH in Dresden, Germany. www.isotope.com | [email protected] 3 Cambridge Isotope Laboratories, Inc. General Information Cambridge Isotope Laboratories, Inc. Facilities Cambridge Isotope Laboratories, Inc. (CIL) has state-of-the-art production facilities for cGMP and non-cGMP manufacturing at its locations in Andover and Tewksbury, Massachusetts. CIL WORLD HEADQUARTERS AND cGMP PRODUCTION LABORATORIES TEWKSBURY, MA USA Cambridge Isotope Laboratories, Inc. moved into its new Tewksbury, Massachusetts facility in the spring of 2013. As our new corporate headquarters, this 40,000-square-foot facility houses our executive team as well as our sales, marketing, finance, regulatory affairs and cGMP production staff. In addition to corporate office space, the facility has a 10,000- square-foot state-of-the-art cGMP suite which includes production laboratories, dedicated isolation rooms, a dedicated analytical laboratory, a packaging laboratory and a development laboratory. CIL PRODUCTION LABORATORIES ANDOVER, MA USA CIL’s primary production facility in Andover, Massachusetts is dedicated to the manufacture of deuterated NMR solvents, stable isotope-labeled chemicals and gases, as well as specific cGMP products. This 57,000-square-foot facility is home to our operations staff and our production and quality-control teams. The formulations group has years of experience formulating highly purified labeled materials into high quality quantitative solutions as analytical standards, either as single-component products or multi-component mixes and calibration solutions. The quality-control lab is equipped with a wide array of instrumentation, including gas chromatograph / mass spectrometers (GC / MS), high field NMRs, HPLCs and an FT-IR. CIL’s chemistry laboratories are equipped with apparatus for both large scale (50+ liters) and microscale chemistry, including equipment for high pressure gas reactions, pH and temperature-controlled enzyme chemistry, high resolution distillation processes and catalytic reduction with both hydrogen and deuterium. The production laboratories are also equipped with analytical equipment for in-process testing, including GC-FID, GC-ECD and HPLC with UV, RI, ELSD and MS detectors. All of these resources allow CIL to consistently produce products with high chemical and isotopic purity. 4 tel: +1-978-749-8000 | 800-322-1174 (USA) | fax: +1-978-749-2768 isotope.com General Information CIL ISOTOPE SEPARATIONS, LLC (CIS) CIS CARBON-13 ISOTOPE CIS OXYGEN-18 ISOTOPE FACILITY, XENIA, OH USA SEPARATION FACILITY SEPARATION FACILITY CIL is the world leader in the separation CIL is recognized as the world leader in the In 2000, CIL responded to the worldwide of 13C and 18O. CIL separates both 13C separation of 13C. In the 1980s, CIL took the shortage of 18O water by embarking on and 18O at its Xenia, OH, facility and has initiative to construct the world’s largest 13C the construction of the world’s largest 18O the world’s largest production capacity isotope-separation plant in order to provide isotope-separation facility. Responding for both 13C and 18O. CIL also has the a sufficient supply of 13C starting materials to the increasing needs of the nuclear only non-governmental D2O enrichment to support new research and diagnostic medicine community, CIL has increased columns in the world located at its developments. In 2013, CIL embarked on a production capacity of 18O multiple times CIS facility. significant expansion of its 13C production since 2000. With each expansion, CIL has capacity and maintains its leadership maintained its position as the world’s position in the separation of 13C. leading producer of 18O. CIL’s 18O water is used in Positron Emission Tomography (PET) and energy-expenditure studies. EURISO-TOP CIL CANADA, INC. ABX GMBH SACLAY, FRANCE MONTREAL, CANADA DRESDEN, GERMANY Euriso-Top is Europe’s leading producer of CIL Canada, Inc. is CIL‘s biotech laboratory ABX is the world’s leading supplier of deuterated