10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 PLK1 / PLK-1 Antibody Rabbit Polyclonal Antibody Catalog # ALS13257 Specification PLK1 / PLK-1 Antibody - Product Information Application WB, IHC Primary Accession P53350 Reactivity Human Host Rabbit Clonality Polyclonal Calculated MW 68kDa KDa PLK1 / PLK-1 Antibody - Additional Information Gene ID 5347 Sample(30 ug whole cell lysate). A: Raji. Other Names 7.5% SDS PAGE. PLK1 antibody diluted at Serine/threonine-protein kinase PLK1, 1:1000. 2.7.11.21, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13, PLK1, PLK Target/Specificity Human PLK1. Predicted cross-reactivity based on amino acid sequence homology: mouse (95%), rat (95%), bovine (95%). Reconstitution & Storage Aliquot and store at -20°C. Minimize freezing and thawing. Precautions PLK1 / PLK-1 Antibody is for research use only and not for use in diagnostic or Anti-PLK1 antibody IHC of human brain, therapeutic procedures. cortex. PLK1 / PLK-1 Antibody - Protein Information Name PLK1 Synonyms PLK Function Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the Page 1/6 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 removal of cohesins from chromosome Anti-PLK1 antibody IHC of human placenta. arms, the inactivation of anaphase- promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGO1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, WEE1 and HNRNPU. Plays a key role in centrosome functions and the Anti-PLK1 antibody IHC of human skin. assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL. NEDD1 phosphorylation promotes PLK1 / PLK-1 Antibody - Background subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, Serine/threonine-protein kinase that performs an important step for spindle formation. several important functions throughout M Phosphorylation of NINL component of the phase of the cell cycle, including the regulation centrosome leads to NINL dissociation from of centrosome maturation and spindle other centrosomal proteins. Involved in assembly, the removal of cohesins from mitosis exit and cytokinesis by chromosome arms, the inactivation of phosphorylating CEP55, ECT2, anaphase-promoting complex/cyclosome KIF20A/MKLP2, CENPU, PRC1 and RACGAP1. (APC/C) inhibitors, and the regulation of mitotic Recruited at the central spindle by exit and cytokinesis. Polo-like kinase proteins phosphorylating and docking PRC1 and acts by binding and phosphorylating proteins KIF20A/MKLP2; creates its own docking sites are that already phosphorylated on a specific on PRC1 and KIF20A/MKLP2 by mediating motif recognized by the POLO box domains. phosphorylation of sites subsequently Phosphorylates BORA, BUB1B/BUBR1, CCNB1, recognized by the POLO box domains. CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, Phosphorylates RACGAP1, thereby creating FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, a docking site for the Rho GTP exchange NPM1, NUDC, PKMYT1/MYT1, KIZ, factor ECT2 that is essential for the PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, cleavage furrow formation. Promotes the SGOL1, STAG2/SA2, TEX14, TOPORS, central spindle recruitment of ECT2. Plays a central role in G2/M transition of mitotic cell p73/TP73, TPT1 and WEE1. Plays a key role in cycle by phosphorylating CCNB1, CDC25C, centrosome functions and the assembly of FOXM1, CENPU, PKMYT1/MYT1, bipolar spindles by phosphorylating KIZ, PPP1R12A/MYPT1 and WEE1. Part of a NEDD1 and NINL. NEDD1 phosphorylation regulatory circuit that promotes the promotes subsequent targeting of the activation of CDK1 by phosphorylating the gamma-tubulin ring complex (gTuRC) to the positive regulator CDC25C and inhibiting centrosome, an important step for spindle the negative regulators WEE1 and formation. Phosphorylation of NINL component PKMYT1/MYT1. Also acts by mediating of the centrosome leads to NINL dissociation phosphorylation of cyclin-B1 (CCNB1) on from other centrosomal proteins. Involved in centrosomes in prophase. Phosphorylates mitosis exit and cytokinesis by phosphorylating FOXM1, a key mitotic transcription CEP55, ECT2, KIF20A/MKLP2, CENPU, PRC1 and regulator, leading to enhance FOXM1 RACGAP1. Recruited at the central spindle by transcriptional activity. Involved in phosphorylating and docking PRC1 and kinetochore functions and sister chromatid KIF20A/MKLP2; creates its own docking sites on cohesion by phosphorylating BUB1B/BUBR1, PRC1 and KIF20A/MKLP2 by mediating FBXO5/EMI1 and STAG2/SA2. PLK1 is high phosphorylation of sites subsequently Page 2/6 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 on non-attached kinetochores suggesting a recognized by the POLO box domains. role of PLK1 in kinetochore attachment or in Phosphorylates RACGAP1, thereby creating a spindle assembly checkpoint (SAC) docking site for the Rho GTP exchange factor regulation. Required for kinetochore ECT2 that is essential for the cleavage furrow localization of BUB1B. Regulates the formation. Promotes the central spindle dissociation of cohesin from chromosomes recruitment of ECT2. Plays a central role in by phosphorylating cohesin subunits such G2/M transition of mitotic cell cycle by as STAG2/SA2. Phosphorylates SGO1: phosphorylating CCNB1, CDC25C, FOXM1, required for spindle pole localization of CENPU, PKMYT1/MYT1, PPP1R12A/MYPT1 and isoform 3 of SGO1 and plays a role in WEE1. Part of a regulatory circuit that regulating its centriole cohesion function. promotes the activation of CDK1 by Mediates phosphorylation of FBXO5/EMI1, a phosphorylating the positive regulator CDC25C negative regulator of the APC/C complex and inhibiting the negative regulators WEE1 during prophase, leading to FBXO5/EMI1 and PKMYT1/MYT1. Also acts by mediating ubiquitination and degradation by the proteasome. Acts as a negative regulator of phosphorylation of cyclin-B1 (CCNB1) on p53 family members: phosphorylates centrosomes in prophase. Phosphorylates TOPORS, leading to inhibit the sumoylation FOXM1, a key mitotic transcription regulator, of p53/TP53 and simultaneously enhance leading to enhance FOXM1 transcriptional the ubiquitination and subsequent activity. Involved in kinetochore functions and degradation of p53/TP53. Phosphorylates sister chromatid cohesion by phosphorylating the transactivation domain of the BUB1B/BUBR1, FBXO5/EMI1 and STAG2/SA2. transcription factor p73/TP73, leading to PLK1 is high on non-attached kinetochores inhibit p73/TP73-mediated transcriptional suggesting a role of PLK1 in kinetochore activation and pro-apoptotic functions. attachment or in spindle assembly checkpoint Phosphorylates BORA, and thereby (SAC) regulation. Required for kinetochore promotes the degradation of BORA. localization of BUB1B. Regulates the Contributes to the regulation of AURKA dissociation of cohesin from chromosomes by function. Also required for recovery after phosphorylating cohesin subunits such as DNA damage checkpoint and entry into STAG2/SA2. Phosphorylates SGOL1: required mitosis. Phosphorylates MISP, leading to for spindle pole localization of isoform 3 of stabilization of cortical and astral SGOL1 and plays a role in regulating its microtubule attachments required for centriole cohesion function. Mediates proper spindle positioning (PubMed:<a href phosphorylation of FBXO5/EMI1, a negative ="http://www.uniprot.org/citations/8991084 regulator of the APC/C complex during " target="_blank">8991084</a>, prophase, leading to FBXO5/EMI1 PubMed:<a href="http://www.uniprot.org/ci ubiquitination and degradation by the tations/11202906" proteasome. Acts as a negative regulator of target="_blank">11202906</a>, p53 family members: phosphorylates TOPORS, PubMed:<a href="http://www.uniprot.org/ci tations/12207013" leading to inhibit the sumoylation of p53/TP53 target="_blank">12207013</a>, and simultaneously enhance the ubiquitination PubMed:<a href="http://www.uniprot.org/ci and subsequent degradation of p53/TP53. tations/12447691" Phosphorylates the transactivation domain of target="_blank">12447691</a>, the transcription factor p73/TP73, leading to PubMed:<a href="http://www.uniprot.org/ci inhibit p73/TP73-mediated transcriptional tations/12524548" activation and pro-apoptotic functions. target="_blank">12524548</a>, Phosphorylates BORA, and thereby promotes PubMed:<a href="http://www.uniprot.org/ci the degradation of BORA. Contributes to the tations/12738781" regulation of AURKA function. Also required for target="_blank">12738781</a>, recovery after DNA damage checkpoint and PubMed:<a href="http://www.uniprot.org/ci entry into mitosis. Phosphorylates MISP, tations/12852856" leading to stabilization of cortical and astral target="_blank">12852856</a>, microtubule attachments required for proper PubMed:<a href="http://www.uniprot.org/ci spindle positioning. tations/12939256" target="_blank">12939256</a>, PLK1 / PLK-1 Antibody - References Page 3/6 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 PubMed:<a href="http://www.uniprot.org/ci