National Institute of Immunology New Delhi Scientific Reports for the Annual Meeting of Research Area Panel & Scientific Advisory Committee April 7 & 8, 2015 (for limited circulation only) Contents Page Program 4 Publications and Patents 7 Research Reports 1. Plasmodium proteins involved in virulence and host Agam P Singh 219 modulation: Host-parasite interactions in Plasmodium liver stages 2. Genetic and functional analyses of host and HIV-1 Akhil C Banerjea 188 genes that affect progression of HIV-1 and development of nucleic acid based antiviral approaches 3. Understanding the regulation of intracellular transport: Amitabha 21 Role of GTPases Mukhopadhyay 4. Studies on immune response from antigen loaded Amulya K Panda 57 biodegradable polymer particles and protein refolding from inclusion bodies 5. Study of mucosal immune response Anna George 16 6. Analysis of Salmonella typhi-host cell interaction Ayub Qadri 107 7. Molecular basis of B cell responses Devinder Sehgal 112 8. Understanding the role of IRFs in dendritic cell Prafullakumar 210 development and innate immunity Tailor 9. Disorders of proliferation: Analysis of novel pathways Rahul Pal 129 and targets 10. Study of genetic and immune factors associated with Rajni Rani 134 autoimmune disorders: Type 1 diabetes and vitiligo 11. Study of immunotherapeutic potential of MIP and the Sangeeta Bhaskar 157 underlying mechanisms in animal models of tuberculosis and tumor 12. Analysis of antigen processing and presentation Satyajit Rath 63 13. Fine tunings of NF-κΒ signaling Soumen Basak 31 14. Biology of T lymphocytes Vineeta Bal 99 15. Cellular and molecular biology of human cancer Anil Suri 91 16. Study on expansion and plasticity of bone marrow stem Asok 26 cells Mukhopadhyay 17. Cell death regulation Chandrima Shaha 72 18. Cellular and molecular aspects of reproduction and viral Satish K Gupta 84 infections 19. Studies of sertoli cells and spermatogonial stem cells of Subeer S 162 the testis and other endocrinology related research Majumdar 20. Molecular mechanism of enzymatic reactions and Apurba K Sau 197 enzyme-ligand interactions 21. Structural and functional biology of mycobacterial Bichitra K Biswal 214 proteins 22. Molecular modelling of proteins and protein-ligand Debasisa Mohanty 44 complexes using knowledge-based approaches and all atom simulations 23. Structure, interaction and design studies involving Dinakar M Salunke 50 regulatory peptides and proteins 24. Ribonucleases and heat shock proteins: involvement in Janendra K Batra 38 host defense 25. Role of carbohydrates in modulating the structure and Kanwal J Kaur 152 function of glycopeptides 26. Structural studies on proteins, dynamics and ligand Monica Sundd 183 interactions using NMR 27. To develop strategies for making sensors and actuators Pramod K 117 for biological processes Upadhyay 28. Protease-catalyzed splicing of peptide bond Rajendra P Roy 68 29. Therapeutic interventions in chronic diseases Sarika Gupta 192 30. Chemical Glycobiology : Glycoform modulation, S Gopalan 229 carbohydrate-based drug design, and glycomics Sampathkumar 31. Biophysical and biochemical characterization of Vidya Raghunathan 226 Leishmania phosphoglycerate kinase: An enzyme in the glycolytic pathway of parasitic protozoa 32. Elucidating the molecular mechanisms of aging and Arnab 178 innate immunity using Caenorhabditis elegans as a Mukhopadhyay model system 33. Molecular biology of infectious diseases Lalit C Garg 206 34. Epigenetic regulation of the eukaryotic genome: Role of Madhulika 104 transcriptional insulators in organizing chromatin Srivastava 35. Role of cell signaling in eukaryotic development Pushkar Sharma 124 36. Reconstructing the chemico-cellular trestle to decipher Rajesh S Gokhale 54 biology of tuberculosis and vitiligo 37. Determining the signaling and repair pathways that are Sagar Sengupta 147 altered in human cancer 38. Understanding the regulation of DNA replication Sandeep Saxena 140 39. The role of tumor suppressors in stress response Sanjeev Das 172 40. Characterization of Minisatellite tagged mRNA Sher Ali 77 transcripts from the human sperm genome 41. Deciphering the role of cell signaling in M. tuberculosis Vinay K 201 biology Nandicoori 42. Production of transgenic and other animal models for Subeer S Majumdar 167 biomedical research (Ancillary activity) Program (April 7-8, 2015) April 7 (Seminar I) 09:15 Opening remarks Prof M Vijayan 09:30 Director‟s address Dr Chandrima Shaha 09:50 Tea (Central foyer) 10:15 Presentation by Principal Investigators The presentations are divided in two groups and will run as parallel sessions in Seminar I and Board Room respectively. Group I (Seminar Room I) Group II (Board Room) Prof M Vijayan, Prof G Padmanaban, Prof Prof Ashok Venkitaraman, Dr Ranjan Sen, Anand Bachhawat, Prof V Nagaraja, Prof Prof Subrata Sinha, Prof Saumitra Das, Dr Umesh Varshney, Dr R Sankaranarayanan, Apurva Sarin, Prof Gagandeep Kang, Dr R Nagaraj, Dr Uttam Surana, Prof DN Prof K Natarajan, Dr Shubhada Rao Chiplunkar, Prof Ramesh NK Bamezai 10:15 Arnab Mukhopadhyay 10:15 Amitabha Mukhopadhyay 11:00 Debasisa Mohanty 11:00 Vineeta Bal 11:45 Bichitra K Biswal 11:45 Sagar Sengupta 12:30 Janendra K Batra 12:30 Sanjeev Das 1:15 - 2:15 PM Lunch 02:15 Vinay K Nandicoori 02:15 Ayub Qadri 03:00 Agam P Singh 03:00 Akhil C Banerjea 03:45 Lalit C Garg 03:45 Asok Mukhopadhyay 04:30 Pramod K Upadhyay 04:30 Soumen Basak 05:15 Amulya K Panda 05:15 Sher Ali Dinner, 6:30 PM onwards (Director's Residence Lawn) April 8 (New Guest House) 10:00 Meeting with RAP/SAC Members, Director and Coordinators 12:00 Lunch and conclusion of the meeting 4 Review of reports by designated RAP/SAC members Reviewer Principal Investigators Page S Gopalan Sampathkumar 229 Prof M Vijayan Dinakar M Salunke 50 Vidya Raghunathan 226 Chandrima Shaha 72 Prof G Padmanaban Apurba K Sau 197 Pushkar Sharma 124 Sandeep Saxena 140 Prafullakumar B Tailor 210 Rahul Pal 129 Prof Ashok Venkitaraman Sarika Gupta 192 Subeer Majumdar 162, 167 Anil Suri 91 Anna George 16 Devinder Sehgal 112 Dr Ranjan Sen Madhulika Srivastava 104 Sandeep Saxena 140 Satyajit Rath 63 Kanwaljit Kaur 152 Sarika Gupta 192 Dr Uttam Surana Subeer Majumdar 162, 167 Anil Suri 91 Rajesh S Gokhale 54 Pushkar Sharma 124 Prof Subrata Sinha Rahul Pal 129 Satish K Gupta 84 Anil Suri 91 Monica Sundd 183 Dr Anand Bachhawat RP Roy 68 Rajesh S Gokhale 54 Chandrima Shaha 72 Prof V Nagaraja Kanwal J Kaur 152 Pushkar Sharma 124 Sangeeta Bhaskar 157 5 Reviewer Principal Investigators Page Chandrima Shaha 72 Prof Umesh Varshney Madhulika Srivastava 104 Sangeeta Bhaskar 157 Sarika Gupta 192 Prof Saumitra Das Satish K Gupta 84 Satyajit Rath 63 Apurba K Sau 197 Prof DN Rao RP Roy 68 S Gopalan Sampathkumar 229 Anna George 16 Dr Apurva Sarin Devinder Sehgal 112 Prafullakumar Tailor 210 Satyajit Rath 63 Rahul Pal 129 Dr Shubhada Chiplunkar Rajni Rani 134 Subeer Majumdar 162, 167 RP Roy 68 Dr R Nagaraj Kanwal J Kaur 152 Monica Sundd 183 Vidya Raghunathan 226 Apurba K Sau 197 Dr Rajan S Gopalan Sampatkumar 229 Sankaranarayanan Monica Sundd 183 Vidya Raghunathan 226 Dinakar M Salunke 50 104 Madhulika Srivastava Prof RNK Bamezai Rajni Rani 134 Sandeep Saxena 140 Anna George 16 112 Prof K Natarajan Devinder Sehgal Prafullakumar Tailor 210 Dinakar M Salunke 50 Rajni Rani 134 Prof Gagandeep Kang Sangeeta Bhaskar 157 Satish K Gupta 84 6 Publications and Patents Original peer-reviewed articles 1. Agarwal S, Parashar D, Gupta N, Jagadish N, Thakar A, Suri V, Kumar R, Gupta A, Ansari AS, Lohiya NK, Suri A (2014) Sperm associated antigen 9 (SPAG9) expression and humoral response in benign and malignant salivary gland tumors. Oncoimmunol 3:12, e974382. 2. Ahmad J, Mir SR, Hohli K, Chuttani K, Mishra AK, Panda, AK, Amin S (2014) Solid nano emulsion preconcentrate for oral delivery of paclitaxel: Formulation design, bio- distribution and scintigraphy imaging. Biomed Res Int 2014: 984756. 3. Almaden JV, Tsui R, Liu Y-C, Birnbaum H, Shokhirev MN, Ngo K, Davis-Turak J, Otero D, Basak S, Rickert RC, Hoffmann A (2014) A pathway switch directs BAFF signaling to distinct NFB transcription factors in maturing and proliferating B cells. Cell Rep 9: 2098–2111. 4. Anand R, Shanka, J, Tiwary BN, Singh AP (2015) Aspergillus flavus induces granulomatous cerebral aspergillosis in mice with display of distinct cytokine profile. Cytokine 72: 166-172. 5. Anish C, Upadhyay AK, Sehgal D, Panda AK (2014) Influences of process and formulation parameters on powder flow properties and immunogenicity of spray dried polymer particles entrapping recombinant Pneumococcal surface protein A. Int J Pharm 466: 198-210. 6. Arora S, Verma S, Banerjea AC (2014) HIV-1 Vpr redirects host ubiquitination pathway. J Virol 88: 9141-9152. 7. Ash D, Subramanian M, Surolia A, Shaha C (2015) Nitric oxide is the key mediator of death induced by fisetin in human acute monocytic leukemia cells. Am J Cancer Res 5:481-497. 8. Bhatia B, Ponia SK, Solanki AK, Dixit A, Garg LC (2014) Identification of glutamate ABC-Transporter component in Clostridium perfringens as a putative drug target. Bioinformation 10: 401-405. 9. Bhatia B, Solanki AK, Kaushik H, Dixit A, Garg LC (2014) B-cell epitope of beta toxin of Clostridium perfringens genetically conjugated to a carrier protein: Expression, purification and characterization of the chimeric protein. Prot Expr Purif 102: 38-44. 10. Bhatnagar P, Patnaik S, Srivastava AK, Mudiam MKR, Shukla Y, Panda AK, Pant AB, Kumar P, Gupta KC (2014) Anti-cancer acitivity of Bromelain nanoparticles by oral administration. J Biomed Nanotech 10: 3558-3578. 11. Bhattacharya I, Gautam M, Majumdar SS (2015) The effect of IBMX and hormones on gene expression by rat Sertoli cells, J Reprod Health Med 1:29-40. 12. Bhattacharya I, Basu S, Sarda K, Gautam M, Nagarajan P, Pradhan BS, Sarkar H, Devi YS, Majumdar SS (2015) Low levels of Gs and Ric8b in testicular sertoli cells 7 may underlie restricted FSH action during infancy in primates Endocrinology 156: 1143-55.