RESEARCHER PROFILES Daniel Bernstein, MD Associate Dean for Curriculum and Scholarship Stanford University School of Medicine Alfred Woodley Salter and Mabel G. Salter Endowed Professor of Pediatrics (Cardiology) Stanford University Former Division Chief, Pediatric Cardiology Former Director, Children’s Heart Center, Lucile Packard Children’s Hospital at Stanford EMAIL [email protected] PROFILE med.stanford.edu/profiles/Daniel-Bernstein LAB murinecvcore.stanford.edu CURRENT RESEARCH EDUCATION/TRAINING Our recent work has focused on the mechanism by which mutations in sarcomeric proteins such MD New York University as myosin lead to the clinical phenotypes of hypertrophic cardiomyopathy (HCM). Utilizing human induced pluripotent stem cell-derived cardiomyocytes, with mutations induced by CRISPR/Cas9 PEDIATRICS RESIDENCY Montefiore Medical Center gene editing, we are undertaking a multi-scale approach ranging from structural and function studies on the single myosin molecule, to the individual myofibril, to whole cells and to microengineered MEDICAL EDUCATION FELLOWSHIP Albert Einstein College of Medicine tissues. To better understand cardiomyocyte mechano-transduction, we are applying FRET sensors in critical sarcomeric and junctional proteins. We are also studying a large biobank of myocardial PEDIATRIC CARDIOLOGY FELLOWSHIP UCSF samples from patients with HCM, combining transcriptomics and metabolomics with measurements of mitochondrial function to determine the degree to which HCM is a disease of altered cardiac BOARD CERTIFICATION Pediatrics, ABP energetics. These studies will allow us to correlate findings from hiPSC-CMs with actual patient samples. Another focus of our lab has been on the molecular mechanisms of RV hypertrophy Pediatric Cardiology, ABP and its transition to RV failure, and how this differs from LV failure. I am also involved in several CLINICAL FOCUS clinical/translational projects: a multi-center clinical study to evaluate novel biomarkers for post- Pediatric Cardiology transplant lymphoproliferative disorder in pediatric solid organ transplant patients; the Pediatric Pediatric Heart Failure and Cardiac Genomics Consortium, an NIH initiative to sequence 10,000 trios for genes associated with Transplantation congenital heart disease; and a study to use immune profiling to predict adverse outcomes after HONORS & AWARDS pediatric left ventricular assist device (LVAD) implantation. CHAIR Committee on Curriculum and Academic SELECTED PUBLICATIONS Policy -Cardiac Myosin Hypertrophic Cardiomyopathy Mutations Release Sequestered Heads and Increase CVI Education & Training Committee Enzymatic Activity. Adhikari AS, Trivedi DV, Sarkar SS, Song D, Kooiker KB, Bernstein D, Spudich JA, β Nature Commun., Best Lecture Award, Stanford University Ruppel KM. 10: 2685, 2019. Medical School (2013) Drp1/Fis1 Interaction Mediates Mitochondrial Dysfunction in Septic Cardiomyopathy. Haileselassie Leo M. Davidoff Society Award for B, Mukherjee R, Joshi AU, Napier BA, Massis LM, Ostberg NP, Queliconi BB, Monack D, Bernstein D, excellence in teaching, Albert Einstein Mochly-Rosen D. J. Mol. Cell. Cardiol., 130: 160-169, 2019. College of Medicine (1982) Functional Assays to Screen and Dissect Genomic Hits: Doubling Down on the National Investment HUME FACULTY SCHOLAR Stanford in Genomic Research. Musunuru K, Bernstein D, Cole FS, Khokha M, Lee F, Lin S, McDonald T, University (1987-88, 1989-90) Moskowitz I, Quertermous T, Sankaran V, Schwartz D, Silverman E, Zhou X, Hasan A, Luo J. MELLON FOUNDATION FELLOW Circulation: Genomic and Precision Medicine, 11:e002178, 2018. Stanford University (1986-87) Physiological Mitochondrial Fragmentation is a Cardiac Adaptation to Increased Demand. Coronado FORMER PRESIDENT M, Zhao M, Fajardo G, Jung G, Gottlieb R, Bernstein D. Circulation Research, 122: 282-295, 2018. Society for Pediatric Research ELECTED MEMBER miR-21 is Associated With Fibrosis and Right Ventricular Failure. Reddy S, Hu D-Q, Zhao M, Blay American Pediatric Society E, Sandeep N, Ong S-G, Jung G, Kooiker KB, Coronado M, Fajardo G, Bernstein D. JCI Insight, 2(9): e91625, 2017. 38 Stanford Cardiovascular Institute RESEARCHER PROFILES Vinicio A. de Jesus Perez, MD, FAHA, FCCP, ATSF Associate Professor of Medicine with Tenure - Division of Pulmonary, Allergy and Critical Care Medicine Co-Director, Stanford Translational Investigator Program Co-Chair, DOM Diversity and Inclusion Committee Chair, PHA PH Care for All Committee Chair, International Pulmonary Vascular Diseases Consortium (iPVD) Vice-chair, 3CPR Scientific & Clinical Education Lifelong Learning Committee EMAIL [email protected] PROFILE profiles.stanford.edu/vinicio-de-jesus-perez EDUCATION/TRAINING WEB pulmonary.stanford.edu; wallcenter.stanford.edu LAB http://med.stanford.edu/dejesusperezlab/home.html MD University of Puerto Rico MEDICINE RESIDENCY Massachusetts General Hospital CURRENT RESEARCH PULMONARY DISEASES FELLOWSHIP My lab focuses on understanding the genetic, cellular and molecular mechanisms University of Colorado involved in the pathogenesis of pulmonary arterial hypertension (PAH). We are interested Stanford University in understanding how pulmonary arteries respond to injury and identify novel genetic PULMONARY VASCULAR FELLOWSHIP modifiers whose dysfunction can trigger small vessel loss and vascular remodeling in Stanford University PAH patients. In particular, we are currently focused on exploring how the Wnt signaling BOARD CERTIFICATION pathways regulate the behavior of pulmonary artery endothelial cells (PAECs), smooth Internal Medicine, ABIM muscle cells (PASMCs) and pericytes in response to injury and whether mutations related Pulmonary Diseases, ABIM to these pathways can affect signaling via other pathways relevant to PAH resulting in Critical Care Medicine, ABIM development of clinical disease. The overarching goal of our work is to identify potential CLINICAL FOCUS biomarkers and drug targets that can be used in the development of novel diagnostic and Pulmonary Hypertension treatment approaches to offer patients afflicted with this devastating disease. Scleroderma Related Lung Diseases Drug Induced Pulmonary Hypertension Pulmonary Fibrosis Life is too unpredictable to plan ahead: You should HONORS & AWARDS be prepared to be surprised every step of the way. American Society of Clinical Investigation (ASCI) membership SELECTED PUBLICATIONS Pulmonary Hypertension Association Loss of Endothelial Derived WNT5a is Associated With Reduced Pericyte Recruitment and Small Vessel Award for Outstanding K08 Loss in Pulmonary Arterial Hypertension. Ke Yuan, Elya Shamskhou, Mark Orcholski, Abinaya Nathan, Be Heard Rare Challenge Disease Sushma Reddy, Hiroaki Honda, Vigneshwaran Mani, Yitian Zeng, Mehmet Ozen, Lingli Wang, Utkan International Award Demirci, Wen Tian, Mark Nicolls and Vinicio A. de Jesus Perez. Circulation. 139(14):1710-1724 (2019). FELLOW, American College of Chest Physicians Hydrogel-based Delivery of Il-10 Improves Treatment of Bleomycin-induced Lung Fibrosis in Mice. FELLOW, American Heart Association Elya Shamskhou, Michael Kratochvil, Mark Orcholski, Nadine Nagy, Gernot Kaber, Emily Steen, Swathi FELLOW, American Thoracic Society Balaji, Ke Yuan, Sundeep Keswani, Ben Danielson, Max Gao, Carlos Medina, Abinaya Nathan, Ananya Keystone Symposia Fellowship Chakraborty, Paul L. Bollyky and Vinicio A. de Jesus Perez. Biomaterials. 203:52-62 (2019). American Society of Clinical Invest. A Unique Collateral Artery Development Program Promotes Neonatal Heart Regeneration. Soumyashree Young Physician Scientist Award ATS Pulmonary Circulation Assembly Das, Andrew B. Goldstone, Hanjay Wang, Justin Farry, Gaetano D’amato, Michael J. Paulsen, Anahita Early Career award) Eskandari, Camille E. Hironaka, Ragini Phansalkar, Bikram Sharma, Siyeon Rhee, Elya A. Shamskhou, Dritan Agalliu, Vinicio A. de Jesus Perez, Y. Joseph Woo and Kristy Red-Horse Cell. 176(5):1128-1142 (2019). EDITORIAL BOARD American Journal of Respiratory Beyond the Lungs: Systemic Manifestations of Pulmonary Arterial Hypertension. Nils P. Nickel, Ke and Critical Care Medicine, Pulmonary Yuan, Peter Dorfmuller, Stevee Provencher, Charly Lai, Sebastien Bonnet, Eric Austin, Carl Koch, Circulation , Circulation Research, AJP Allison Morris, Frederic Perros, David Montani, Roham Zamanian and Vinicio A. de Jesus Perez. Lung Cell Molecular Biology, PLoS One American journal of respiratory and critical care medicine, 2019 (in press). 48 Stanford Cardiovascular Institute RESEARCHER PROFILES Michael Fischbein, MD, PhD Associate Professor of Cardiothoracic Surgery (Adult Cardiac Surgery) Director of Thoracic Aortic Surgery Program Director, Department of Cardiothoracic Surgery EMAIL [email protected] PROFILE med.stanford.edu/profiles/Michael-Fischbein EDUCATION/TRAINING CURRENT RESEARCH MD Boston University (1995) Our group is interested the molecular and genetic mechanisms of aortic aneurysm/ PHD UCLA (2001) dissection development, and the molecular mechanisms of aneurysm formation in Marfan RESIDENCY UCLA (2003) Syndrome. Clinical research interests include thoracic aortic diseases (aneurysms, FELLOWSHIP Stanford University (2006) dissections). BOARD CERTIFICATION Thoracic Surgery, American Board of Thoracic Surgery, SELECTED PUBLICATIONS General Surgery, American Board of Surgery Anatomically Specific Reactive Oxygen Species Production Participates in Marfan Syndrome Aneurysm Formation. Emrich F, Penov K, Arakawa M, Dhablania N, Burdon G, Pedroza AJ, CLINICAL FOCUS Koyano TK, Kim