<p>Mitochondrial dynamics, mitophagy, and autophagy </p><p>Mitochondrial fusion and fission pathways <br>Mitochondrial import protein pathway </p><p>iΔΨm, (OXPHOSh) (Cell differentiationi) </p><p>Ca<sup style="top: -0.2975em;">2+ </sup></p><p>Fusion </p><p><sup style="top: -0.3929em;">++++</sup>N </p><p>Internal signal sequence </p><p>β-barrel outer membrane precursors </p><p>Opa1 </p><p>SH SH </p><p>ER </p><p>Mfn1/2 </p><p>SH SH </p><p>GTP <br>GTP </p><p>GTP <br>GTP </p><p>GTP </p><p>C<br>Inner membrane and </p><p>Matrix carrier precursors </p><p>(N-terminal presequences) </p><p>Outer membrane fusion </p><p>22 <br>70 </p><p>GTP </p><p>35 <br>37 <br>20 </p><p>6<br>5</p><p>Sam complex </p><p>Opa1 </p><p>Tom complex <br>Cytosol </p><p>Outer membrane </p><p>Sam50 <br>7</p><p>DRP1 </p><p>Inner membrane fusion <br>Fis1 </p><p>Mdm10 <br>Tim8-Tim13 </p><p>Bax/Bak apoptosis </p><p>Tom40 </p><p>HR2 regions </p><p>S-S S-S </p><p>Tim9-Tim10 <br>Erv1 </p><p>95 A </p><p>Inner mitochondrial space </p><p>54 </p><p>S-S </p><p>Reorganization sequestration </p><p>Mia40 </p><p>Fission </p><p>21 <br>50 </p><p>18 <br>18 </p><p>Translocation to meet ATP needs <br>Depolarization </p><p>Tim22 </p><p>iΔΨm </p><p></p><ul style="display: flex;"><li style="flex:1">KREBS Cycle </li><li style="flex:1">NADH FADH2 </li></ul><p></p><p>Tim23 <br>44 </p><p>Oxa1 </p><p>17 </p><p>Tim 22 complex </p><p></p><ul style="display: flex;"><li style="flex:1">Mba1 </li><li style="flex:1">Mdm </li></ul><p></p><p>38 </p><p>Mia </p><p>I<br>ATP <br>H+ </p><p>H+ H+ <br>II III IV H+ </p><p>17 </p><p>ADP - Pi </p><p>+++ </p><p>16 </p><p>Oxa </p><p>mtHsp70 </p><p>ATP </p><p>+++ </p><p>MPP </p><p>Mge1 </p><p></p><ul style="display: flex;"><li style="flex:1">Healthy mitochondrion </li><li style="flex:1">Mitophagy </li></ul><p></p><p>Matrix <br>Tim 23 complex </p><p></p><ul style="display: flex;"><li style="flex:1">Mitophagy pathway </li><li style="flex:1">Autophagy pathway </li></ul><p></p><p>Lysosomal hydroiase </p><p>Lysosome </p><p>Hypoxia, Nutrient/Growth&nbsp;Rapamycin Starvation&nbsp;condition factor deprivation </p><p>Ubiquitin <br>Cytosol </p><p>Parkin </p><p>Lamp </p><p>Damaged mitochondrion </p><p>Parkin </p><p>Stress </p><p>Parkin <br>Mfn1 </p><p>PINK1 </p><p>iΔΨm </p><p>Parkin VDAC1 <br>MARF </p><p>PINK1 <br>PINK1 </p><p>mTOR <br>AMPK </p><p>Parkin <br>Mfn2 </p><p>PINK1 <br>PINK1 </p><p>Autophagolyosome </p><ul style="display: flex;"><li style="flex:1">Phagophore </li><li style="flex:1">Autophagosome </li></ul><p></p><p>PARL </p><p>LC3-II </p><p>Parkin FIS1 </p><p></p><ul style="display: flex;"><li style="flex:1">PINK1-L </li><li style="flex:1">PINK1-S </li></ul><p></p><p>P</p><p></p><ul style="display: flex;"><li style="flex:1">ATG13 </li><li style="flex:1">ULK </li></ul><p>LC3-II </p><p>Membrane </p><p>FIP200 </p><p></p><ul style="display: flex;"><li style="flex:1">PINK1 </li><li style="flex:1">PINK1 </li></ul><p>PINK1 </p><p>MIRO2 <br>Parkin </p><p>P</p><p>Bak Parkin </p><p>PINK1 </p><p>MIRO1 Parkin </p><p>Cytoplasmic macromolecule </p><p></p><ul style="display: flex;"><li style="flex:1">Cytosol </li><li style="flex:1">proLC3 </li></ul><p>Atg4B </p><p>LC3-II </p><p>Organelle </p><p></p><ul style="display: flex;"><li style="flex:1">Atg7/LC3-I </li><li style="flex:1">Atg5/Atg12/Atg16 </li></ul><p>PE </p><p>Atg9 <br>Atg18 <br>Atg2 </p><p>Atg5/Atg12/Atg16 </p><p>LC3-II </p><p></p><ul style="display: flex;"><li style="flex:1">LC3-I </li><li style="flex:1">Atg3/LC3-I </li><li style="flex:1">LC3-II </li></ul><p></p><p>LC3-II <br>LC3-II </p><p>Mitochondrion </p><p>Autophagosome formation </p><ul style="display: flex;"><li style="flex:1">Autophagosome </li><li style="flex:1">Autolysosome </li></ul><p></p><p>P13K III Beclin1 <br>Atg16L <br>Atg12 <br>Atg5 </p><p>PE </p><p>LC3-II p150 </p><p>Atg14 </p><p>Fusion </p><p>LC3-II <br>LC3-II </p><ul style="display: flex;"><li style="flex:1">Atg10 </li><li style="flex:1">Atg16L </li></ul><p>Atg5 <br>Atg12 </p><p>Phosphatidyl-ethanolamine deactivation </p><p>p62 </p><p>Atg5 <br>Atg12 </p><p>LC3-II </p><p>Cathepsin </p><p>HDAC6 </p><p>Bcl-2 <br>Atg10 </p><p>Mfn1 </p><p>Cathepsin </p><p>LC3-I <br>Atg3 </p><p>MARF <br>Mfn2 </p><p>Wortmannin <br>3-Methyladenine </p><p></p><ul style="display: flex;"><li style="flex:1">Atg7 </li><li style="flex:1">Atg5 </li></ul><p></p><p>p62 </p><p>Atg10 <br>Atg12 </p><p>VDAC1 </p><p>Atg3 <br>LC3-I <br>Atg7 <br>Atg7 Atg10 </p><p>Lipase </p><p>Cathepsin </p><p>Lipase </p><p>Lysosome </p><p>Atg7 </p><ul style="display: flex;"><li style="flex:1">Atg12 </li><li style="flex:1">Atg7 </li></ul><p>LC3-I </p><p>Atg7 </p><ul style="display: flex;"><li style="flex:1">Atg12 </li><li style="flex:1">Atg4 </li></ul><p></p><p>LC3 </p><p>To find out more, please visit </p><p><a href="/goto?url=http://www.abcam.com/mitochondrialdynamics" target="_blank">www.abcam.com/mitochondrialdynamics </a></p><p>Copyright © 2018 Abcam, All rights reserved. This poster was made in collaboration with Michelangelo Campanella, Department of Comprarative Biomedical Sciences, The&nbsp;Royal Veterinary College, University of London. </p><p>Mitochondrial dynamics, mitophagy, and autophagy </p><p>Mitochondrial fusion and fission pathways <br>Mitochondrial import protein pathway </p><p>iΔΨm, (OXPHOSh) (Cell differentiationi) </p><p>Ca<sup style="top: -0.1309em;">2+ </sup></p><p>Fusion </p><p><sup style="top: -0.1729em;">++++</sup>N </p><p>Internal signal sequence </p><p>β-barrel outer membrane precursors </p><p>Opa1 </p><p>SH SH </p><p>ER </p><p>Mfn1/2 </p><p>SH SH </p><p>GTP GTP </p><p>GTP GTP </p><p>GTP </p><p>C<br>Inner membrane and </p><p>Matrix carrier precursors </p><p>(N-terminal presequences) </p><p>Outer membrane fusion </p><p></p><ul style="display: flex;"><li style="flex:1">22 </li><li style="flex:1">70 </li></ul><p></p><p>GTP </p><p>35 <br>37 <br>20 </p><p>6<br>5</p><p>Sam complex </p><p>Opa1 </p><p>Tom complex <br>Cytosol </p><p>Outer membrane </p><p>Sam50 <br>7</p><p>DRP1 </p><p>Inner membrane fusion <br>Fis1 </p><p>Mdm10 <br>Tim8-Tim13 </p><p>Bax/Bak apoptosis </p><p>Tom40 </p><p>HR2 regions </p><p>S-S S-S </p><p>Tim9-Tim10 <br>Erv1 </p><p>95 A </p><p>Inner mitochondrial space </p><p>54 </p><p>S-S </p><p>Reorganization sequestration </p><p>Mia40 </p><p>Fission </p><p>21 <br>50 </p><p>18 <br>18 </p><p>Translocation to meet ATP needs <br>Depolarization </p><p>Tim22 </p><p>iΔΨm </p><p></p><ul style="display: flex;"><li style="flex:1">KREBS Cycle </li><li style="flex:1">NADH FADH2 </li></ul><p></p><p>Tim23 <br>44 </p><p>Oxa1 </p><p>17 </p><p>Tim 22 complex </p><p></p><ul style="display: flex;"><li style="flex:1">Mba1 </li><li style="flex:1">Mdm </li></ul><p>38 </p><p>Mia </p><p>I<br>ATP <br>H+ </p><p>H+ H+ <br>II III IV H+ </p><p>17 </p><p>ADP - Pi </p><p>+++ </p><p>16 </p><p>Oxa </p><p>mtHsp70 </p><p>ATP </p><p>+++ </p><p>MPP </p><p>Mge1 </p><p></p><ul style="display: flex;"><li style="flex:1">Healthy mitochondrion </li><li style="flex:1">Mitophagy </li></ul><p></p><p></p><ul style="display: flex;"><li style="flex:1">Matrix </li><li style="flex:1">Tim 23 complex </li></ul><p></p><p></p><ul style="display: flex;"><li style="flex:1">Mitophagy pathway </li><li style="flex:1">Autophagy pathway </li></ul><p></p><p>Lysosomal hydroiase </p><p>Lysosome </p><p>Hypoxia, Nutrient/Growth&nbsp;Rapamycin Starvation&nbsp;condition factor deprivation </p><p>Ubiquitin <br>Cytosol </p><p>Parkin </p><p>Lamp </p><p>Damaged mitochondrion </p><p>Parkin </p><p>Stress </p><p>Parkin Mfn1 </p><p>PINK1 </p><p>iΔΨm </p><p>Parkin VDAC1 <br>MARF </p><p></p><ul style="display: flex;"><li style="flex:1">PINK1 </li><li style="flex:1">PINK1 </li></ul><p></p><p>mTOR <br>AMPK </p><p>Parkin Mfn2 </p><p>PINK1 <br>PINK1 </p><p>Autophagolyosome </p><ul style="display: flex;"><li style="flex:1">Phagophore </li><li style="flex:1">Autophagosome </li></ul><p></p><p>PARL </p><p>LC3-II </p><p>Parkin FIS1 </p><p></p><ul style="display: flex;"><li style="flex:1">PINK1-L </li><li style="flex:1">PINK1-S </li></ul><p></p><p>P</p><p></p><ul style="display: flex;"><li style="flex:1">ATG13 </li><li style="flex:1">ULK </li></ul><p>LC3-II </p><p>Membrane </p><p>FIP200 </p><p></p><ul style="display: flex;"><li style="flex:1">PINK1 </li><li style="flex:1">PINK1 </li></ul><p>PINK1 </p><p>MIRO2 Parkin </p><p>P</p><p>Bak Parkin </p><p>PINK1 </p><p>MIRO1 Parkin </p><p>Cytoplasmic macromolecule </p><p></p><ul style="display: flex;"><li style="flex:1">Cytosol </li><li style="flex:1">proLC3 </li></ul><p>Atg4B </p><p>LC3-II </p><p>Organelle </p><p></p><ul style="display: flex;"><li style="flex:1">Atg7/LC3-I </li><li style="flex:1">Atg5/Atg12/Atg16 </li></ul><p>PE </p><p>Atg9 </p><ul style="display: flex;"><li style="flex:1">Atg18 </li><li style="flex:1">Atg2 </li></ul><p></p><p>Atg5/Atg12/Atg16 </p><p>LC3-II </p><p></p><ul style="display: flex;"><li style="flex:1">LC3-I </li><li style="flex:1">Atg3/LC3-I </li><li style="flex:1">LC3-II </li></ul><p></p><p>LC3-II <br>LC3-II </p><p>Mitochondrion </p><p>Autophagosome formation </p><ul style="display: flex;"><li style="flex:1">Autophagosome </li><li style="flex:1">Autolysosome </li></ul><p></p><p>P13K III Beclin1 <br>Atg16L Atg12 Atg5 </p><p>PE </p><p>LC3-II p150 </p><p>Atg14 </p><p>Fusion </p><p>LC3-II <br>LC3-II </p><ul style="display: flex;"><li style="flex:1">Atg10 </li><li style="flex:1">Atg16L </li></ul><p>Atg5 Atg12 </p><p>Phosphatidyl-ethanolamine deactivation </p><p>p62 </p><p>Atg5 Atg12 </p><p>LC3-II </p><p>Cathepsin </p><p>HDAC6 </p><p>Bcl-2 </p><p>Mfn1 </p><p>Atg10 </p><p>Cathepsin </p><p>LC3-I Atg3 </p><p>MARF <br>Mfn2 </p><p>Wortmannin <br>3-Methyladenine </p><p></p><ul style="display: flex;"><li style="flex:1">Atg7 </li><li style="flex:1">Atg5 </li></ul><p></p><p>p62 </p><p>Atg10 Atg12 </p><p>VDAC1 </p><p>Atg3 <br>LC3-I Atg7 <br>Atg7 Atg10 </p><p>Lipase </p><p>Cathepsin </p><p>Lipase </p><p>Lysosome </p><p>Atg7 </p><ul style="display: flex;"><li style="flex:1">Atg12 </li><li style="flex:1">Atg7 </li></ul><p>LC3-I </p><p>Atg7 </p><ul style="display: flex;"><li style="flex:1">Atg12 </li><li style="flex:1">Atg4 </li></ul><p>LC3 </p><p>To find out more, please visit </p><p><a href="/goto?url=http://www.abcam.com/mitochondrialdynamics" target="_blank">www.abcam.com/mitochondrialdynamics </a></p><p>Copyright © 2018 Abcam, All rights reserved. This poster was made in collaboration with Michelangelo Campanella, Department of Comprarative Biomedical Sciences, The&nbsp;Royal Veterinary College, University of London. </p>