Anti-IPO7 Mouse mAb Catalog # PTM-5910 General Information Images Host species Mouse WB Clonality Recombinant monoclonal Blocking buffer: 5% NFDM/TBST Clone number JMMR-1765 Primary ab dilution: 1:1000 Primary ab incubation condition: room Synonym RANBP7 temperature 2h Immunogen UniProt MW (kDa) Applications Secondary ab: Goat Anti-Mouse IgG H&L species (HRP) Human O95373 120 WB, IHC-P Lysate: HeLa, K562, N2a, Rat brain Protein loading quantity: 20 μg Product Usage Information Exposure time: 60 s Predicted MW: 120 kDa Application Dilution Recommended species Observed MW: 120 kDa WB 1:500-1:1000 Human, Mouse, Rat IHC-P IHC-P 1:50-1:100 Human Tissue: Human breast cancer Section type: Formalin fixed & Paraffin - Properties embedded section Storage Store at -20°C. Avoid freeze / thaw cycles. Retrieval method: High temperature and high pressure Stability Stable for 12 months from date of receipt / reconstitution Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Constituents PBS, Glycerol, BSA Primary ab incubation condition: 1 hour at Purity Protein G purified room temperature Secondary ab: Anti-Rabbit and Mouse Isotype IgG1/Kappa Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Target Information Comment: Color brown is the positive signal for PTM-5910 Function Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter- Research Use like protein in association with the importin-beta subunit For research use only, not for use in diagnostic procedures. KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. Cellular location Cytoplasm, Nucleus APPLICATIONS WB: Western blot IP: Immunoprecipitation CONTACT US China Global IHC-P: Immunohistochemistry-Paraffin ChIP: Chromatin Immunoprecipitation Tel: 400-100-1145 +1 312-802-6843 ICC/IF: Immunocytochemistry/Immunofluorescence FC: Flow Cytometry Email: [email protected] [email protected] Web: www.ptm-biolab.com.cn www.ptmbiolabs.com .