THE CITY OF SAN DIEGO 2003 QUALITY ASSURANCE MANUAL Bulla gouldiana Pilsbry 1895 1.0 em OCEAN MONITORING PROGRAM METROPOLITAN WASTEWATER DEPARTMENT ENVIRONMENTAL MONITORING AND TECHNICAL SERVICES DIVISION 600 B Str«t,Suite 500, San Dl<ao. CA t!IOI-4517 QUALITY ASSURANCE MANUAL CITY OF SAN DIEGO OCEAN MONITORING PROGRAM REVISED JANUARY 2004 CREDITS AND ACKNOWLEDGMENTS .................................................................................................................... iii INTRODUCTION .................................................................................................................................................................. 1 GENERAL INTRODUCTION ................................................................................................................................................ 1 LABORATORY STRUCTURE .............................................................................................................................................. 2 Marine Biology and Ocean Operations ................................................................................................................... 2 Data Management and Reporting Group ............................................................................................................... 2 Information Technology and GIS Systems Group ................................................................................................. 2 Ocean Operations and Toxicology Group .............................................................................................................. 2 Taxonomy Group ............................................................................................................................................................. 3 Marine Microbiology and Vector Management ..................................................................................................... 3 Marine Microbiology Group ...................................................................................................................................... 3 Vector Management Group .......................................................................................................................................... 3 PROFESSIONALISM AND TRAINING ............................................................................................................................. 3 FIELD SAMPLING PROCEDURES ............................................................................................................................... 5 Logging Procedures and Sample Custody .............................................................................................................. 5 Shore Station Samples .................................................................................................................................................. 5 Offshore Samples ........................................................................................................................................................... 5 Navigation and Station Location at Offshore Stations ..................................................................................... 5 Field Records ................................................................................................................................................................... 9 Collection of Water Quality Samples ....................................................................................................................... 9 Collection of Oil and Grease and Suspended Solids Samples ......................................................................... 9 Measurement of Transparency .................................................................................................................................... 10 Water Column Profiling ................................................................................................................................................ 10 Macrofaunal Community Samples ............................................................................................................................ 10 Sediment Quality Samples ........................................................................................................................................... 11 Demersal Fishes and Megabenthic Invertebrates ................................................................................................ 11 Collection of Tissue Burden Samples ...................................................................................................................... 12 LABORATORY AND ANALYTICAL PROCEDURES ............................................................................................ 13 Microbiological Analyses ............................................................................................................................................. 13 Heterotrophic Plate Count .......................................................................................................................................... 13 Multiple Tube Fermentation ....................................................................................................................................... 13 Membrane Filtration ..................................................................................................................................................... 14 Colilert-18 and Enterolert (IDEXX) ........................................................................................................................ 14 Vitek Verification ............................................................................................................................................................ 14 Coliphage ......................................................................................................................................................................... 15 Microbiological Quality Assurance Procedures .................................................................................................. 15 Macrofaunal Community Analyses ........................................................................................................................... 16 Voucher Collection ........................................................................................................................................................ 17 Interagency Taxonomic Standardization ................................................................................................................ 17 Taxonomic Training ....................................................................................................................................................... 17 Macrofaunal Quality Assurance Procedure .......................................................................................................... 19 Resorts ................................................................................................................................................................................ 19 Biomass Determinations ............................................................................................................................................... 19 Reidentifications ............................................................................................................................................................. 19 Data Review ..................................................................................................................................................................... 19 i Fish Tissue Burden Analyses ..................................................................................................................................... 21 Preparation ...................................................................................................................................................................... 21 Dissection .......................................................................................................................................................................... 21 Bioassay Laboratory Standard Operation Procedures ....................................................................................... 22 DATA MANAGEMENT........................................................................................................................................................ 23 Data Review Procedures ............................................................................................................................................... 23 Data Entry Procedures .................................................................................................................................................. 23 Data Analysis and Reporting ...................................................................................................................................... 25 RESULTS OF QUALITY ASSURANCE PROCEDURES CONDUCTED DURING 2003 ...................... 27 CTD Inter-calibration Exercise ................................................................................................................................. 27 Bacteriological Quality Assurance Analyses ....................................................................................................... 30 Macrofaunal Community – Resort Analysis .......................................................................................................... 30 LITERATURE CITED .........................................................................................................................................................