FISHERIES RESEARCH BOARD OF CANADA Translation Series No. 1413 Influence of ozidized oil and vitamin E on the culture of yellowtail. By H. Sakaguchi and A. Hamaguchi . Original title: Sanka yu tenka shiryo ni yoru hamachi no shiiku to bitamin E tenka no Koka. From: Nihon Suisan Gakkai-Shi (Bulletin of the Japanese Society of Scientific Fisheries), 35 (12): 1207-1214, 1969. Translated by the Translation Bureau (RK) Foreign Languages Division Department of the Secretary, of State of Canada Fisheries Research Board of Canada Halifax Laboratory Halifax, N.S. 1970 22 pages typescript Fee DEPARTMENT OF THE SECRETARY OF STATE SECRÉTARIAT D'ÉTAT . TRANSLATION BUREAU BUREAU DES TRADUCTIONS FOREIGN LANGUAGES DIVISION DES LANGUES DIVISION CANADA ÉTRANGÈRES TRANSLATED FROM'- TRADUCTION DE Japanese English • AUTHoR - AUTEUR . H.Sakaguchi Hamaguchi TITLE IN ENGLISH«,.- TITRE ANGLAIS • • Sanka.yu tenkehiryo ni yoru.hamachi no shiiku to bitamin ' -d'tenka, no ttiie 'in foreign:44eguagia (tranàliterate foreign oharactera) :Treluence. oftjçieized Oil anct , Vitamin , E on the Culture of . RVURENCE IN Foftgtori ‘,ANGUAgE (MARE OF ISOOK OR PUBLICATION) IN FULL. TRANSLITERATE FoREIGN CHARACTERS. •IREFERENCE EN LANGUE ETRANGERE (NOM Du LIVRE Ou PUBLICATION), Au COMPLET. TRANSCRIRE EN CARACTERES PHONETIQUEs. Nippon Suisan Gakkai-Shi REOERENCE. IN ENGLISH - RÉFÉRENCE EN ANGLAIS Bulletin oftheJapanese Society of - Scientific'Fisheries, PUBLISH ER - iorrEurt PAGE.NUMBERS IN ORIGINAL DATE OF PUBLICATION • • NumER0S DES PAGES DANS Nippàn sp,isan etkkal DATE DE PUBLICATION L , 0RIGINAL 1207-1214 YEfte ISSLIE.No. VOLUME ANNEE NUMERO • PLACE OF, PUBLICATION NUMBER OF TYPED PAGES . 0E4 . .DE PUBLICAtION NOMBRE DE PAGeS DACTYLOGRAPHIÉES 1969 35 12 22 REQUEfTING DEPAFITMENT naberipa:aaaarch Board TRANSLATION BUREAU NO.. 0056 MINISTERE.CLIENT, NOTRE DOSSIER No ■ • eke wcH OR DIVISION 4boratorY, Ne. TRANSLATOR (INITIALS) .."piptÉerioN CILI DIVISION TRADUCTEUR (INITIALES) • . . . . , •.• , :i›IERsbN.tegvEsTiNG• -rit a: „tabliez DATE CpMPLETED 20 -4- 70 .. ,-pgNAND ACHEVE LE siPtIRE DOssiEfe UNEDITED DRAFr TRANIIP-MCititi . makion . . Oely • DEOF REQUEST Min& 3.8e 1910 TRADUCTIObl NON bietE DE LA DEMANDE - . ,ñpaQn .,iou-kgneirt . 200 tO en DEPARTMENT OF THE SECRETARY OF STATE SECRÉTARIAT D'ÉTAT TRANSLATION BUREAU BUREAU DES TRADUCTIONS FOREIGN LANGUAGES DIVISION DIVISION DES LANGUES ÉTRANGÈRES CLIENT'S NO. DEPARTMENT DIVISION/BRANCH CITY N° DU CLIENT MINISTERE DIVISION/DIRECTION VILLE Fisheries Research Board Halifax Laboratory Halifax, N.S. BUREAU NO. LANGUAGE TRANSLATOR (INITIALS) DATE • No DU BUREAU LANGUE TRADUCTEUR (INITIALES) 0056 Japanese M. K. Bulletin of the Japanese Society of Scientific Fisheries Vol.35, No.12, 1969. 1207 Influence of Oxidized Oil and Vitamin E on the Culture of Yellowtail Hiromi Sakaguchi and Akira Hamaguchi** In the previous paper1) , the effect of antioxidant treated sand-eel .as food for yellowtail was reported. Thereafter, we investigated the effects of oxidized oil and vitamin E administration on yellowtail fed with artificial diets. Fish were fed for 93 days with diets containing 8.5% of fresh or oxidized cod liver oil to which DL-etocopheryl acetate was either added or not (Table 1).. The weight gains of the 'fish are shown in Fig. 1 and Table 2. The fish fed with oXidized oil, without the addition ! 0 ‘LU of vitamin E, showed a slightly poor appetite after about • • (") (ji 40 days • — . Cy. of feeding, and at the termination of experiment a (72. 1.7.: •••• i; leaning of the dorsal muscle was observed on about 10% of the 0 fish. These symptoms are probably due to muscle dystrophy as 0 reported with the carp12) (Fig.2). However such symptoms were ( : 1 not found e -- < in the vitamin E supplemented group. I. The muscle of fish fed with oxidized oil but without ** (Fish. Exp. 5 . Hyogo Pref., Nakasaki-machi, Akashi, Japan) SOS:-200-10-31 the addition Of vitamin E showed a sli,rhtly higher TBA value than that of the vitamin E supplemented fish, but there was no extreme difference in the TBA values of the hepatopancreas of these two groups (Table 3). At the termination of experiment, the glycogen contents in the hepatopancreas of groups 1, 2, and 4 were elevated to about twice_compared to its content at 70th . day. However, such elevation of the'glycogen level was not seen in the group fed with oxidized oil. The activities of transaminases (GOT, GPT) were higher in the fish fed with oxidized oil than the fish fed with fresh oil and these enzyme activities could not be reduced even by the administration of vitamin E. The alkaline phosphatase activity showed little, if any, difference between these groups (Table 4). 3. Death believed caused by oxidized oil, as well as being observed in other fish, can be seen quite frequently. during the rearing of yellowtail . Sakaguchi et ai' investi- gated the effect of antioxidant-treated sand-eel as food for yellowtail and reported that there was no ill effect on the growth of yellowtail if the peroxide value of fat in the feed was below 50. Furukawa et al 2 used sand-eel which was kept improperly cooled for one year as feed to raise yellowtail. The result of their experiment showed that the oxidized oil poisoned yellowtail, however, the poisoning was prevented by the addition of vitamine E to the food. The author has raised yellowtail using oxidized cod liver oil in order to see the influence of oxidized oil on the growth of yellowtail. We have obtained severa l . observations as a result of the experiment, on the drawbacks caused by the oxidized oil and the effect of vitamine E on preventing the drawbacks caused by said oil. Experiment Fish and the method of raising, The fish used were Seriola Quinqueradiate TEMMINCK et SCHLEGEL and they were obtained from the same group of fish as those used in the previous report. 3 The average weight and the average length of the fish at the beginning of the experiment were 43g and 13.2cm respectively. The experiment 4 was started on July 20, 1968 and continued for three months. The test groups were made up of four separate groups, namely the group fed sand-eel (group 1, comparative group), the group fed pure cod'liver oil added to the assorted feed (group 2, standard feed group), the group fed assorted feed with oxidized cod liver oil added (group 3) end the group fed on the _same feed system as 3 except vitamine E is added (group 4) The feed compositions are .shown in Table 1. The combined feed was made up of fish meal, gluten, glucose, • vitamine mixture and mineral mixture with pure cod liver oil, oxidized cod liver oil, etc., have been added. The vitamine mixture was the same as the mixture used in the comparative group in the previous report 3 except vitamine E was not contained in the mixture used in this experiment. The cod liver oil used was the pure sample with a peroxide value of less than 13. This cod liver oil was used in the second group - without modifying the composition but the oil was oxidized by passing air through the oil at room temperature for 260-290 hours so that the resulting compound had a peroxide value of between 480-500 before the oil was used for groups 3 and 4. The vitamine content in the feed system of-the second and third groups prior to the addition of oil was 1.3me. The feed system of the fourth group was made up by adding vitamine E (a-Tocopheryl acetate 25011)/g) to the feed system of the third . group. The amount of vitamine added to the feed system was kept at 0.6% for the first 71 days and the amount was changed 5. Table 1. Composition of'experimental diets Group Sand-eel Fish Gluten Glucose Cod liver Vitamin Eineral Jitamin No. meal oil mixture* mixture** E*** 1 100 2 68 17 2.5 ' 8.5++ 3 1 3 68, 17 2.5 8.5+++ 3 1 4 68 17 2.5 8.5 1 ++ 3 1 0. 6 5+ 68 17 2.5 8.5++ 3 1 + Fish from control group Of vitamin test. I.+ Fresh oil (POV 11 e-'13). +++ Oxidized oil (POV 4E0"500). * Same as previous one. In groups 2 to 4, DL-06-tocophery1 acetate was replaced by same amount ofa-starch. ** Saine as Halver's4) . *** .0.6 g(150 TU) of DL-e-tocopheryi acetate was added until 71st day, thereafter increased to 1.2 g, to 1.2% from the 72nd day to the end of the experiment. The mineral mixture was according to Halver. 4 The amount of feed used per day was kept between 2.5-3% (dry weight base) of the weight of the fish tested. The feed was given once in the morning and once in the evening. The weight of the fish was checked after every 10 feedings and the amount of feed was adjusted accordingly. The testing pond was 3.5 x 1.2m and contained 10 tons of water. Four testing ponds of the same dimensions were used in this experiment. Each pond was filled with 150 fish. The water waS filtered with a rapid filtering machine and air was passed through it before passing it into the pond. The flow rate of the water was about 10 tons per hour. However, the flow rate was not controlled after September during the day and sand-filtered water was circulated during the night. The measurement of growth rate and the treatment of test fish The measurement of the body length was done every 10 days. Twenty to thirty fish were taken out Of each group and after anesthetizing them with MS-220 (concentration of 1/10,000) the fish were weighed and the body length was measured.