Comparative Pathology Laboratory Department of Pathology • College of Veterinary Medicine • University of Georgia ® Special Stains Available Pathogens Mycobacteria Acid Fast Gram negative & Gram positive bacteria Lillie Twort; Gimenez Histoplasma capsulatum Giemsa Bacteria: Leptospira sp., Helicobacter sp., CAR bacillus Warthin Starry Fungi GMS, PAS Neural Tissue Axons Bielchowsky's; Bodains Nerve fibers and neurofibrils Holmes Glial fibers in areas of gliosis Holzer Myelin Luxol Fast Blue Argyrophil granules in neurosecretory tumors Grimelius Extracellular Matrix Reticular fibers Gomori's reticulum Elastic Fibers and Mast cells Luna Collagen Masson's trichrome; Movats Pentachrome Elastic Fibers Verhoef van Gieson; Movat's Pentachrome Fibrin PTAH (Phosphotungstic acid hematoxylin); Fraser Lendrum Materials Deposited in Tissues Amyloid Congo Red Copper stores in tissue Copper Melanin Fontana Masson; Schmorl's Glycogen PAS (Periodic acid-Schiff) with and without disease Glycoprotein macromolecules PAS (Periodic acid-Schiff) with and without disease Iron Perl's iron Calcium von Kossa Fat Oil Red O; Sudan IV Hemoglobin Okajima Bile Hall's Mucin Alcain Blue; Mayer's mucicarmine; Movat's Pentachrome; Safranin O Cellular Components DNA Feulgen reaction, Methyl Green Pyronin Y; Toluidin Blue RNA Methyl Green Pyronin Y Nuclei Safrinin O Glomerular basement membrane PAM (Periodic acid-silver methenamine) Mast cell granules Safrinin O; Giemsa Sulfonated polysaccharides Toluidin Blue Myopathic changes (nemaline rods, ragged red fibers) Gomori's trichrome Blood film Giemsa Cellular details in heavily pigmented tissue sections Melanin bleach Phone: 706-202-8608 Email: [email protected] Tamas Nagy, DVM, PhD, DACVP 501 DW Brooks Drive Athens, GA 30602 •TEL.: 706-202-8608 •FAX: 706-542-5828 E-mail: [email protected] Web: http://www.vet.uga.edu/vpp/labs/comparative-pathology.