OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AR09841PU-L Human PPP3R1 / Calcineurin B (1-170, His-tag) - Purified Alternate names: CNA2, CNB, Calcineurin subunit B type 1, PP2B regulatory subunit 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1 Quantity: 0.5 mg Concentration: 1.0 mg/ml (determined by Bradford assay) Background: PPP3R1, also known as Calcineurin subunit B type 1, is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin is composed of two subunits; calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF- AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. Uniprot ID: P63098 NCBI: NP_000936 GeneID: 5534 Species: Human Source: E. coli Format: State: Liquid purified protein Purity: >90% Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl Description: Recombinant human PPP3R1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV Molecular weight: 21.5 kDa (190aa), confirmed by MALDI-TOF Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20121030 1 / 2 AR09841PU-L: Human PPP3R1 / Calcineurin B (1-170, His-tag) - Purified Pictures: Recombinant human PPP3R1, 1-170 aa, His-tagged For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20121030 2 / 2 Powered by TCPDF (www.tcpdf.org).