Barley Genetics Newsletter Volume 37 Editorial Committee P. Bregitzer U. Lundqvist V. Carollo Blake Table of Contents Information about the Barley Genetics Newsletter The Barley Genetics Newsletter is published electronically at http://wheat.pw.usda/gov/ggpages/bgn Contributions for publication in the Barley Genetics Newsletter should be sent to the Technical Editor, Dr. Phil Bregitzer (see Instructions to contributors below for details on submission). Reports on research activities (including ongoing projects, descriptions of new genetic and cytological techniques, and current linkage maps) are invited for the Research Notes section. Researchers are encouraged to submit descriptions of genetic stocks for the Barley Genetic Stocks section. Letters to the editors are welcome and will be published. Please inform the Technical Editor of any errors you notice in this volume or in previous volumes; corrections will be published in future volumes, and electronic copy will be appropriately corrected. All correspondence and contributions to the Barley Genetics Newsletter should be in English. Please include your complete mailing address, including your country, and an e- mail address (if you have one) with all correspondence and contributions. Acknowledgements The contributors of research reports and the diligence of the many Coordinators make this publication possible. Victoria Carollo Blake and the GrainGenes team at the USDA- ARS make possible the electronic version of BGN. Instructions for contributors Volume 37 (2007) of the Barley Genetics Newsletter Submissions will be published via the internet (http://wheat.pw.usda.gov/ggpages/bgn/). Approximately quarterly, new submissions will be appended to existing submissions; the page numbers of existing submissions will not change and citation information will remain constant. BGN 37 will consist of all submissions received prior to the end of the calendar year of 2007, and will be compiled into a printable version that will be available via the Graingenes website. Send submissions to: Dr. Phil Bregitzer, BGN Technical Editor, USDA-ARS, 1691 S. 2700 W., Aberdeen, ID, 83210, USA. Telephone: (voice) 208-397-4162 ext. 116; (fax) 208-397-4165 e-mail: [email protected] Coordinators assigned at the International Barley Genetics Symposium should submit their reports to: Dr. Udda Lundqvist Coordinator, Barley Genetics Newsletter Nordic Genetic Resource Center P.O. Box 41 SE-230 53 Alnarp, Sweden Phone: +46 40 536640 FAX: +46 40 536650 Cell phone: +46 70 624 1502 E-mail: [email protected] Manuscripts should be written in English and be brief. Each paper should be prepared separately, with the title and the authors' names and addresses at the top of the first page. For each paper to be included in Barley Genetics Newsletter Volume 37, submit a file to the Technical Editor in one of the following ways (in order of preference): 1. As an attachment to an e-mail message 2. A 3.5" diskette or CD; include also one high quality printed copy 3. High-quality paper copy (acceptable only in unusual circumstances) Instructions for file preparation Text and tables for each article can be in one file, but place each figure in a separate file. Name these files with your initials, a manuscript number, and "txt" or "fig" (in the case of separate files for text and figures), and a number for each figure (in the case of separate files). For example, the files for a manuscript plus two figures from Phil Bregitzer would be: pb1txt.wpd, pb1fig1.gif , and pb1fig2.gif . File Formats for Text and Tables: Preferred: Word or WordPerfect, PC-compatible formatting. Macintosh formats are not acceptable. Please keep formatting simple and consistent with that of BGN 36. Use Times New Roman Font, 12 pt, with both right and left justification, except for the title and author information and tables, which should be centered. Please use only tabs and hard returns in the preparation of text files. Tables must be prepared using the table feature of your word processing program, and not prepared using tabs. Please do not add additional lines to your table except with the table formatting tools in your word processor. Please use center justification for tables, and not "full" justification (i.e. left and right justification, that is used for text). Figures can be submitted in any common format but avoid the necessity of color for interpretation, to enable printing and copying hardcopy in black and white without losing information contained in the figures. Table of Contents Barley Genetics Newsletter, Volume 37, 2007 [Other volumes of the Barley Genetics Newsletter] Click here for a complete printable version of Volume 37 (Please Note: 2.12 MB) Information about the Barley Genetics Newsletter, p. i Instructions for contributors, p. ii Research Notes Dominance and recessiveness of parameters of Aluminum-resistance of 1 – 4 barley F hybrids at different concentrations of stress factor (.doc, .pdf) 2 F. M. Lisitsyn, I. I. Lisitsyna Frequency distributions and composite interval mapping for QTL analysis in ‘Steptoe’ x ‘Morex’ barley mapping population (.doc, pdf) 5 - 20 H. S. Rao, O. P. Basha, N. K. Singh, K. Sato, H. S. Dhaliqal PCR-based markers targeting barley putative grain yield and quality QTLs regions (.doc, pdf) 21 - 23 I. A. del Blanco, D. A. Schmierer, A. Kleinhofs , S. E. Ullrich Genetic architecture for yield and quality component traits over two environments in barley (Hordeum vulgare L.) (.doc, .pdf) 24 - 28 A. K. Verma, S.R. Vishwakarma and P.K. Singh Line x tester analysis in barley (Hordeum vulgare L.) across environments (.doc, .pdf) 29 - 33 A. K. Verma, S.R. Vishwakarma and P.K. Singh Four new barley mutants (.doc, .pdf) 34 - 36 S.A.I .Wright, M. Azarang, A. B. Falk The Scandinavian barley chlorophyll mutant collection (.doc, .pdf) 37 - 43 M. Hansson CAPS markers targeting barley Rpr1 region (.doc, .pdf) 44 - 46 L. Zhang, A. Kleinhofs Tolerance to high copper ions concentration in the nutrient medium of 47 - 49 some Bulgarian cultivars (.doc, .pdf) J. Stoinova, S. Phileva, M. Merakchyiska, S. Paunova Coordinator's Reports Rules for Nomenclature and Gene Symbolization in Barley (.doc, .pdf) 100 - 104 J. D. Franckowiak, U. Lundqvist Overal Coordinator's Report (.doc, .pdf) 105 - 153 U. Lundqvist Barley Genetic Stocks for 2007 (.doc, .pdf) 153 - 187 L. Dahleen, J.D. Franckowiak, U. Lundqvist Descriptions of Barley Genetic Stocks for 2007 (.doc, .pdf) 188 - 301 L. Dahleen, J.D. Franckowiak, U. Lundqvist Barley Genetics Newsletter 37:1-4 (2007) Dominance and recessiveness of parameters of Aluminum-resistance of barley F2 hybrids at different concentrations of stress factor E. M. Lisitsyn and I. I. Lisitsyna North-East Agricultural Research Institute, 166-а Lenin street, Kirov, 610007, Russia e-mail: [email protected] Till now there is no uniform opinion in the scientific literature about number and type of action of genes coding barley aluminum resistance. For example, Rigin, Yakovleva (2001) considers, that it is controlled by two oligogenes, as a minimum, with possible action of genes with weaker effect. Other authors assume the control of the parameter by one dominant gene Pht [Stolen, Andersen, 1978], or gene Alp [Reid, 1971], both located on chromosome 4 [Minella, Sorrells, 1997]. Gourley et al [1990] concluded, that type of action of genes coding aluminum resistance in sorghum (additive, partially or completely dominant), depends not only on a researched geno- type, but also on used Al concentration. Aniol [1995, 1996] has established, that when the concentration of aluminum in test solution is low (30-40 µМ), cereal cultures (wheat, rye) use mechanisms that block accumulation of aluminum in roots (for example, chelation of aluminum at the expense of exudation of organic acids by root system), at higher concentration of aluminum in root growth environment (200-300 µМ) the basic role is played by other physiological mechanisms of Al-resistance. Thus the author remarks [Aniol, 1997] that the aluminum resistance of wheat plants at a concentration of 296 µМ is controlled by 2 genes and at a concentration of 592 µМ aluminum resistance is controlled by three genes. He wrote, that genes located in D-genome of wheat, are expressed only at high Al concentration, and genes located on chromosome 5А are expressed at all studied concentrations. The aim of our research was to determine the influence of Al concentration on a direction and character of dominance of parameters of roots growth of barley F2 hybrid seedlings. Material and Methods The direct and reciprocal F2 hybrids of four selection numbers of barley (№№ 565-98, 889-93, 999-93 and 1030-93), bred in North-East Agricultural Research Institute (Kirov, Russia) were taken for the analysis. By results of the preliminary laboratory analyses the parental forms of these hybrids differed significantly on a level of Al-resistance that corresponded to the research aim. A level of Al-resistance (relative root length - RRL) was estimated under conditions of rolled culture on five-day barley seedlings according to the technique described earlier [Lisitsyn, 2000] by division of value of root length of each individual seedling in test treatment variant (0.5 and 1.0 mM of aluminum as sulphate salt, рН 4.3) on value of average root length of control variant (without the stress factor, рН 6.0). Each sample volume consists of 99- 105 seedlings in each treatment variant. Character of dominance for parameters of root growth of F2 hybrid plants was estimated by equation [Petr, Frey, 1966]: d = F2 - MP HP-MP 1 Barley Genetics Newsletter 37:1-4 (2007) , where d = degree of dominance; F2, НР, МР = means of F2 hybrids, resistant parent value, and mid parent value, respectively. Results and Discussion Expression of Al-resistance genes appreciably depend on a concentration of aluminum in test solution and with its increase the resistance of all hybrids was reduced without exception (table 1).