10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 MLCK Antibody (N-term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP7966a Specification MLCK Antibody (N-term) - Product Information Application WB,E Primary Accession Q15746 Other Accession P29294 Reactivity Human, Mouse Predicted Rabbit Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Antigen Region 923-953 MLCK Antibody (N-term) - Additional Information Gene ID 4638 Other Names Myosin light chain kinase, smooth muscle, Western blot analysis of anti-MLCK-long Pab MLCK, smMLCK, Kinase-related protein, (Cat. #AP7966a) in mouse brain tissue KRP, Telokin, Myosin light chain kinase, lysate. MLCK-long (Arrow) was detected using smooth muscle, deglutamylated form, purified Pab. Secondary HRP-anti-rabbit was MYLK, MLCK, MLCK1, MYLK1 used for signal visualization with chemiluminescence. Target/Specificity This MLCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-953 amino acids from the N-terminal region of human MLCK. Dilution WB~~1:1000 Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Storage Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C Western blot analysis of lysate from mouse in small aliquots to prevent freeze-thaw bladder tissue lysate, using MLCKlong cycles. Antibody (M1)(Cat. #AP7966a). AP7966a was diluted at 1:1000. A goat anti-rabbit IgG Precautions H&L(HRP) at 1:10000 dilution was used as Page 1/4 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 MLCK Antibody (N-term) is for research use the secondary antibody. Lysate at 35ug. only and not for use in diagnostic or therapeutic procedures. MLCK Antibody (N-term) - Protein Information Name MYLK (HGNC:7590) Synonyms MLCK, MLCK1, MYLK1 Function Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Western blot analysis of lysate from mouse Involved in the inflammatory response (e.g. bladder tissue lysate, using MLCKlong apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, Antibody (M1)(Cat. #AP7966a). AP7966a was airway hyperreactivity and other activities diluted at 1:1000. A goat anti-rabbit IgG relevant to asthma. Required for tonic H&L(HRP) at 1:10000 dilution was used as airway smooth muscle contraction that is the secondary antibody. Lysate at 35ug. necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 MLCK Antibody (M1) (Cat. #AP7966a) channel activity in a calcium-dependent western blot analysis in SK-BR-3 cell line signaling, by inducing its subcellular lysates (35ug/lane).This demonstrates the localization at the plasma membrane. MLCK antibody detected the MLCK protein Promotes cell migration (including tumor (arrow). cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to MLCK Antibody (N-term) - Background trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic MLCK, a member of the Ser/Thr protein kinase nerve head astrocyte migration. Probably family, is a calcium/calmodulin-dependent involved in mitotic cytoskeletal regulation. enzyme responsible for smooth muscle Regulates tight junction probably by contraction via phosphorylation of a specific modulating ZO-1 exchange in the Page 2/4 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 perijunctional actomyosin ring. Mediates serine in the N-terminus of myosin light chains burn-induced microvascular barrier injury; (MLC), an event that facilitates myosin triggers endothelial contraction in the interaction with actin filaments. It is a central development of microvascular determinant in the development of vascular hyperpermeability by phosphorylating MLC. permeability and tissue edema formation. In Essential for intestinal barrier dysfunction. the nervous system it has been shown to Mediates Giardia spp.-mediated reduced control the growth initiation of astrocytic epithelial barrier function during giardiasis processes in culture and to participate in intestinal infection via reorganization of transmitter release at synapses formed cytoskeletal F-actin and tight junctional between cultured sympathetic ganglion cells. ZO-1. Necessary for hypotonicity-induced MLCK acts as a critical participant in signaling Ca(2+) entry and subsequent activation of sequences that result in fibroblast apoptosis. volume-sensitive organic osmolyte/anion Smooth muscle and non-muscle isozymes are channels (VSOAC) in cervical cancer cells. expressed in a wide variety of adult and fetal Responsible for high proliferative ability of breast cancer cells through anti-apoptosis. tissues and in cultured endothelium with qualitative expression appearing to be neither Cellular Location tissue- nor development-specific. Non-muscle Cytoplasm. Cell projection, lamellipodium. isoform 2 is the dominant splice variant Cleavage furrow. Cytoplasm, cytoskeleton, expressed in various tissues. The Telokin stress fiber. Note=Localized to stress fibers isoform, which binds calmodulin, has been during interphase and to the cleavage found in a wide variety of adult and fetal furrow during mitosis tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 Tissue Location fibronectin type III domain and 9 Smooth muscle and non-muscle isozymes immunoglobulin-like C2-type domains. are expressed in a wide variety of adult and fetal tissues and in cultured endothelium MLCK Antibody (N-term) - References with qualitative expression appearing to be neither tissue- nor development-specific. Lazar, V., et al., Genomics 57(2):256-267 Non-muscle isoform 2 is the dominant (1999). Watterson, D.M., et al., J. Cell. splice variant expressed in various tissues. Biochem. 75(3):481-491 (1999). Garcia, J.G., et Telokin has been found in a wide variety of al., Am. J. Respir. Cell Mol. Biol. 16(5):489-494 adult and fetal tissues. Accumulates in well (1997). Potier, M.C., et al., Genomics differentiated enterocytes of the intestinal 29(3):562-570 (1995). epithelium in response to tumor necrosis factor (TNF). MLCK Antibody (N-term) - Protocols Provided below are standard protocols that you may find useful for product applications. • Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture MLCK Antibody (N-term) - Citations P2Y6 receptor inhibition aggravates ischemic brain injury by reducing microglial phagocytosis P2Y6 Receptor-Mediated Microglial Phagocytosis in Radiation-Induced Brain Injury. Page 3/4 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Page 4/4 Powered by TCPDF (www.tcpdf.org).