Project title: Improving integrated pest management in strawberry Project title: Improving integrated pest management in strawberry Project number: SF 156 Project leader: Michelle Fountain, NIAB-EMR, New Road, East Malling, Kent ME19 6BJ Report: Year 3 Annual report, March 2018 Previous report: Year 2 Annual Report, March 2017 Key staff: Jerry Cross, Jean Fitzgerald, Chantelle Jay, Phil Brain, Adrian Harris, Luca Csokay, Francesco Maria Rogai, Glen Powell (NIAB-EMR); Steve Edgington, (CABI); William Kirk, (Keele University); Clare Sampson (Russell IPM); David Hall, Dudley Farman (NRI); Tom Pope, Juliane Graham, Rosie Homer, Rob Graham, Charlotte Rowley (Harper Adams University); Robert Irving (ADAS), Neil Audsley (Fera) Location of project: NIAB EMR Industry Representative: Louise Sutherland, Freiston Associates Ltd. Date project commenced: 01 April 2015 Date project completed 31 March 2020 (or expected completion date) 1 DISCLAIMER DISCLAIMER While the Agriculture and Horticulture Development Board seeks to ensure that the information contained within this document is accurate at the time of printing, no warranty is given in respect thereof and, to the maximum extent permitted by law the Agriculture and Horticulture Development Board accepts no liability for loss, damage or injury howsoever caused (including that caused by negligence) or suffered directly or indirectly in relation to information and opinions contained in or omitted from this document. © Agriculture and Horticulture Development Board [2018]. No part of this publication may be reproduced in any material form (including by photocopy or storage in any medium by electronic mean) or any copy or adaptation stored, published or distributed (by physical, electronic or other means) without prior permission in writing of the Agriculture and Horticulture Development Board, other than by reproduction in an unmodified form for the sole purpose of use as an information resource when the Agriculture and Horticulture Development Board or AHDB Horticulture is clearly acknowledged as the source, or in accordance with the provisions of the Copyright, Designs and Patents Act 1988. All rights reserved. All other trademarks, logos and brand names contained in this publication are the trademarks of their respective holders. No rights are granted without the prior written permission of the relevant owners. [The results and conclusions in this report are based on an investigation conducted over a one-year period. The conditions under which the experiments were carried out and the results have been reported in detail and with accuracy. However, because of the biological nature of the work it must be borne in mind that different circumstances and conditions could produce different results. Therefore, care must be taken with interpretation of the results, especially if they are used as the basis for commercial product recommendations.] 2 AUTHENTICATION We declare that this work was done under our supervision according to the procedures described herein and that the report represents a true and accurate record of the results obtained. Michelle Fountain Deputy Head of Pest and Pathogen Ecology NIAB EMR, New Road, East Malling, Kent ME19 6BJ Signature ............................................................ Date ...06 April 2018... Report authorised by: Louise Sutherland, Industry Representative Freiston Associates Ltd. Signature ............................................................ Date .................... 3 Table of Contents GROWER SUMMARY 8 Western flower thrips 9 Objective 1 - Develop effective biological methods for managing western flower thrips, Frankliniella occidentalis (WFT), compatible with pesticide use against SWD, improve the reliability of biocontrol of WFT with predatory mites, and develop effective approaches to the use of entomopathogenic fungi (EPF) for control of WFT. ....................................................................................................................... 9 Integrating pesticides with phytoseiid mites 14 Objective 2 - Refine pest control programmes on strawberry, integrating pesticides with phytoseiid mites. ............................................................................................ 14 IPM controls for capsids and blossom weevil 16 Objective 3. Develop IPM compatible controls for European tarnished plant bug (Lygus rugulipennis), common green capsid (Lygocoris pabulinus) and strawberry blossom weevil (Anthonomus rubi) ....................................................................... 16 Potato aphid 19 Objective 4. Improve insecticide control of the potato aphid, Macrosiphum euphorbiae, so as to be more compatible with IPM programmes. ........................ 19 Aphid control 23 Objective 5. Improve control of aphids through the growing season. .................... 23 SCIENCE SECTION 26 Objective 1. Develop effective biological methods for managing western flower thrips, Frankliniella occidentalis (WFT), compatible with pesticide use for control of spotted wing drosophila, Drosophila suzukii (SWD) 26 1.1 Develop and determine the efficacy and ease of use of the prototype extraction device for WFT and the predatory mite N. cucumeris in commercial strawberry crops, by agronomist and growers 26 Introduction ........................................................................................................... 26 Methods ................................................................................................................ 27 4 Results .................................................................................................................. 31 Discussion ............................................................................................................ 36 Future Work .......................................................................................................... 37 Objective 1.2. Determine the distribution of Neoseiulus cucumeris on commercial strawberry plants after their introduction for WFT management 38 Introduction ........................................................................................................... 38 Methods ................................................................................................................ 38 Results and Discussion ........................................................................................ 40 Conclusions .......................................................................................................... 52 Introduction ........................................................................................................... 53 Methods ................................................................................................................ 53 Results .................................................................................................................. 55 Conclusions .......................................................................................................... 59 1.2. Making applications of entomopathogenic fungi (EPF) effective for control of WFT 60 Objective 2. Refine pest control programmes on strawberry, integrating pesticides with phytoseiid mites. ............................................................................................ 61 Task 2.1. In field, effect of insecticides commonly used to target spring aphids on the establishment of N. cucumeris, aphids and parasitoids 61 Introduction ........................................................................................................... 61 Objective 3.Develop IPM compatible controls for European tarnished plant bug, Lygus rugulipennis, common green capsid, Lygocoris pabulinus, and strawberry blossom weevil, Anthonomus rubi. ....................................................................... 64 Task 3.1. To investigate the potential of a multi-pheromone blue sticky trapping system for Lygus rugulipennis, Lygocoris pabulinus and Frankliniella occidentalis 64 Introduction ........................................................................................................... 64 5 Objectives ............................................................................................................. 64 Methods ................................................................................................................ 65 Results .................................................................................................................. 69 Discussion ............................................................................................................ 79 Future work ........................................................................................................... 80 Task 3.2. To investigate the potential of a push-pull system for control of capsids in strawberry. ............................................................................................................ 81 Introduction ........................................................................................................... 81 Methods ................................................................................................................ 81 Results .................................................................................................................. 87 Conclusions .......................................................................................................... 94 Future Work .........................................................................................................