US010760075B2 ( 12 ) United States Patent ( 10 ) Patent No .: US 10,760,075 B2 Sommer et al . ( 45 ) Date of Patent : Sep. 1 , 2020 ( 54 ) TREATING AND PREVENTING MICROBIAL 8,329,867 B2 12/2012 Lazar et al . INFECTIONS 8,354,509 B2 1/2013 Carven et al . 8,735,553 B1 5/2014 Li et al . 9,701,964 B2 7/2017 Clube et al . ( 71 ) Applicant: SNIPR Biome Aps , Copenhagen Ø 9,758,583 B2 * 9/2017 Wang CO7K 16/2818 ( DK ) 9,822,372 B2 11/2017 Zhang et al . 9,879,269 B2 1/2018 Barrangou et al . ( 72 ) Inventors: Morten Sommer , Copenhagen Ø ( DK ) ; 10,066,233 B2 9/2018 Barrangou et al . Virginia Martinez , Copenhagen Ø 10,136,639 B2 11/2018 Wuest et al . ( DK ) ; Eric Van Der Helm , 10,136,649 B2 11/2018 Barrangou et al . Copenhagen Ø ( DK ) ; Jakob Krause 10,195,273 B2 2/2019 Clube et al. 10,300,138 B2 5/2019 Clube Haaber , Copenhagen Ø ( DK ) ; Ana De 10,300,139 B2 5/2019 Clube Santiago Torio , Copenhagen Ø ( DK ) ; 10,363,308 B2 7/2019 Clube Christian Grøndahl , Copenhagen Ø 10,463,049 B2 11/2019 Clube ( DK ) ; Jasper Clube , London ( GB ) 10,506,812 B2 12/2019 Clube 10,524,477 B2 1/2020 Clube ( 73 ) Assignee : SNIPR Biome ApS , Copenhagen Ø 10,596,255 B2 3/2020 Clube ( DK ) 10,603,379 B2 3/2020 Clube 2004/0096974 A1 5/2004 Herron et al . ( * ) Notice : Subject to any disclaimer , the term of this patent is extended or adjusted under 35 (Continued ) U.S.C. 154 ( b ) by 146 days . FOREIGN PATENT DOCUMENTS ( 21 ) Appl. No .: 15 / 967,484 EP 2 320 940 B1 5/2011 EP 2 840 140 A1 2/2015 ( 22 ) Filed : Apr. 30 , 2018 ( Continued ) ( 65 ) Prior Publication Data OTHER PUBLICATIONS US 2018/0273940 A1 Sep. 27 , 2018 Mayo Clinic , https://www.mayoclinic.org/diseases-conditions/ ( 51 ) Int . Ci . bacterial - vaginosis / symptoms- causes/ syc - 20352279 , accessed Jul. C12N 15/11 ( 2006.01 ) 20 , 2018. * A61P 31/04 ( 2006.01 ) ( Continued ) A61K 39/395 ( 2006.01 ) A61K 38/00 ( 2006.01 ) COZK 14/21 ( 2006.01 ) A61K 38/46 ( 2006.01 ) Primary Examiner Gary B Nickol CO7K 16/28 ( 2006.01 ) Assistant Examiner - Lakia J Jackson - Tongue ( 52 ) U.S. CI . CPC C12N 15/11 ( 2013.01 ) ; A61K 38/465 ( 74 ) Attorney , Agent, or Firm — Morrison & Foerster ( 2013.01 ) ; A61K 39/3955 ( 2013.01 ) ; A61K LLP 39/39558 ( 2013.01 ) ; A61P 31/04 ( 2018.01 ) ; CO7K 16/2818 ( 2013.01 ) ; C07K 16/2827 ( 2013.01 ) ; C12N 2310/20 ( 2017.05 ) ; C12N ( 57 ) ABSTRACT 2320/30 ( 2013.01 ) ; C12N 2320/31 ( 2013.01 ) ; The invention provides methods for treating or preventing YO2A 50/478 ( 2018.01) microbial ( eg , bacterial ) infections and means for perform ( 58 ) Field of Classification Search ing these methods. In particular, treatment of infections ??? .... C12N 15/111 ; C12N 2320/30 , C12N requiring rapid and durable therapy is made possible , such 2320/31 ; A61P 31/04 ; A61K 39/3955 as for treating acute conditions such as septicemia , sepsis , See application file for complete search history . SIRS or septic shock . The invention is particularly useful, ( 56 ) References Cited for example, for treatment of microbes such as for environ mental, food and beverage use . The invention relates inter U.S. PATENT DOCUMENTS alia to methods of controlling microbiologically influenced 4,626,504 A 12/1986 Puhler et al . corrosion ( MIC ) or biofouling of a substrate or fluid in an 4,870,287 A 9/1989 Cole et al . industrial or domestic system . The invention also useful for 5,633,154 A 5/1997 Schaefer et al. the treatment of pathogenic bacterial infections in subjects 5,760,395 A 6/1998 Johnstone receiving a treatment for disease or condition , such as a 5,844,905 A 12/1998 McKay et al . 5,885,796 A 3/1999 Linsley et al. transplant or a treatment for cancer, a viral infection or an 6,207,156 B1 3/2001 Kuchroo et al. autoimmune disease . 8,008,449 B2 8/2011 Korman et al . 8,017,114 B2 9/2011 Korman et al . 8,119,129 B2 2/2012 Jure -Kunkel et al. 19 Claims , 9 Drawing Sheets 8,241,498 B2 8/2012 Summer et al. 8,252,576 B2 8/2012 Campbell et al. Specification includes a Sequence Listing . US 10,760,075 B2 Page 2 ( 56 ) References Cited 2019/0321470 A1 10/2019 Clube et al . 2020/0030444 A1 1/2020 Clube et al . U.S. PATENT DOCUMENTS 2020/0157237 A1 * 5/2020 Regev CO7K 16/2818 2005/0118719 Al 6/2005 Schmidt et al . FOREIGN PATENT DOCUMENTS 2009/0155768 Al 6/2009 Scholl et al . 2010/0093617 Al 4/2010 Barrangou et al. RU 2531343 C2 10/2014 2011/0002889 Al 1/2011 Barrangou et al . WO WO - 1995 / 001994 A1 1/1995 2011/0008369 Al 1/2011 Finnefrock et al . WO WO - 1998 / 042752 A1 10/1998 2011/0136688 A1 6/2011 Scholl et al . WO WO - 2000 / 037504 A2 6/2000 2012/0177645 A1 7/2012 Langermann et al . WO WO - 2000 / 037504 A3 6/2000 2012/0269859 Al 10/2012 Minato WO WO - 2001 / 014424 A2 3/2001 2012/0294796 A1 11/2012 Johnson et al . WO WO - 2001 / 014424 A3 3/2001 2013/0011828 A1 1/2013 Barrangou et al . WO WO - 2005 / 003168 A2 1/2005 2014/0022021 Al 1/2014 Kusachi WO WO - 2005 / 003168 A3 1/2005 2014/0105912 Al 4/2014 Noelle WO WO - 2005 / 009465 Al 2/2005 2014/0107092 Al 4/2014 Meyerson et al . WO WO - 2005 / 046579 A2 5/2005 2014/0199767 A1 7/2014 Barrangou et al. WO WO - 2005 / 046579 A3 5/2005 2014/0234972 A1 8/2014 Zhang WO WO - 2006 / 003179 A2 1/2006 2014/0294898 Al 10/2014 Miller et al . WO WO - 2006 / 003179 A3 1/2006 2014/0341920 A1 11/2014 Noelle WO WO - 2006 / 072625 A2 7/2006 2015/0031134 A1 1/2015 Zhang et al . WO WO - 2006 / 072625 A3 7/2006 2015/0050699 Al 2/2015 Siksnys et al . WO WO - 2006 / 072626 A1 7/2006 2015/0064138 Al 3/2015 Lu et al . WO WO - 2006 / 121168 A1 11/2006 2015/0125463 Al 5/2015 Cogswell et al . WO WO - 2007 / 025097 A2 3/2007 2015/0132419 Al 5/2015 Arvik et al . WO WO - 2007 / 042573 A2 4/2007 2015/0184139 Al 7/2015 Zhang et al . WO WO - 2007 / 042573 A3 4/2007 2015/0353905 A1 12/2015 Weiss WO WO - 2008 / 084106 A2 7/2008 2016/0009805 A1 1/2016 Kowanetz et al . WO WO - 2008 / 084106 A9 7/2008 2016/0009813 A1 1/2016 Themeli WO WO - 2008 / 108989 A2 9/2008 2016/0024510 A1 1/2016 Bikard et al . WO WO - 2008 / 132601 A1 11/2008 2016/0115488 A1 4/2016 Zhang et al . WO WO - 2009 / 044273 A2 4/2009 2016/0115489 Al 4/2016 Zhang et al . WO WO - 2009 / 044273 A3 4/2009 2016/0159905 A1 6/2016 Abdiche WO WO - 2009 / 101611 A1 8/2009 2016/0160186 A1 6/2016 Parsley et al . WO WO - 2009 / 114335 A2 9/2009 2016/0324938 Al 11/2016 Bikard et al . WO WO - 2009 / 114335 A3 9/2009 2016/0333348 Al 11/2016 Clube et al. WO WO - 2010 / 011961 A1 1/2010 2016/0345578 A1 12/2016 Barrangou et al. WO WO - 2010 / 027827 A2 3/2010 2016/0347836 A1 12/2016 Grosso WO WO - 2010 / 027827 A3 3/2010 2016/0354416 Al 12/2016 Gajewski et al . WO WO - 2010 / 065939 Al 6/2010 2017/0022499 Al 1/2017 Lu et al . WO WO - 2010 / 075424 A2 7/2010 2017/0037416 A1 2/2017 Barrangou et al . WO WO - 2011 / 014438 A1 2/2011 2017/0173085 Al 6/2017 Kovarik et al . WO WO - 2011 / 066342 A2 6/2011 2017/0174713 Al * 6/2017 Du C07F 9/65583 WO WO - 2011 / 066342 A3 6/2011 2017/0175142 Al 6/2017 Zhang et al . WO WO - 2012 / 071411 A2 5/2012 2017/0196225 A1 7/2017 Clube et al. WO WO - 2012 / 071411 A3 5/2012 2017/0246221 A1 8/2017 Clube et al . WO WO - 2012 / 160448 A2 11/2012 2017/0304443 Al 10/2017 Lebwohl et al . WO WO - 2012 / 160448 A3 11/2012 2017/0327582 A1 11/2017 Bissonnette et al . WO WO - 2012 / 164565 A1 12/2012 2017/0340733 A1 11/2017 Cao et al . WO WO - 2013 / 006490 A2 1/2013 2018/0015131 A1 1/2018 Gajewski et al . WO WO - 2013 / 006490 A3 1/2013 2018/0055852 A1 3/2018 Kutok et al . WO WO - 2013 / 025779 Al 2/2013 2018/0064114 Al 3/2018 Clube et al . WO WO - 2013 / 067492 A1 5/2013 2018/0064115 A1 3/2018 Clube et al . WO WO - 2013 / 176772 A1 11/2013 2018/0070594 A1 3/2018 Clube et al. WO WO - 2014 / 018423 A2 1/2014 2018/0084785 Al 3/2018 Clube et al . WO WO2014015252 A1 1/2014 2018/0084786 A1 3/2018 Clube et al. WO WO - 2014 / 093595 A1 6/2014 2018/0140698 A1 * 5/2018 Clube A61P 37/00 WO WO - 2014 / 093661 A2 6/2014 2018/0146681 Al 5/2018 Clube et al . WO WO - 2014 / 093661 A3 6/2014 2018/0155721 A1 6/2018 Lu et al . WO WO - 2014 / 093661 A9 6/2014 2018/0155729 Al 6/2018 Beisel et al . WO WO - 2014 / 124226 Al 8/2014 2018/0179547 A1 6/2018 Zhang et al .