CATALOGUE NUMBER 1 GENERAL LABORATORY REAGENTS Table of Contents Analytical—Other 3 Solvents—High Volume 71 Derivatisation 3 Anhydrous 71 Electrochemistry 3 Biotech 71 Microscopy 6 Generic 71 Miscellaneous 7 Histology 81 HPLC 81 Analytical Reagents 13 Chromatography & CE 13 Solvents—Special Qualities & Packaging 84 Derivatisation 16 Anhydrous 84 Spectroscopy 18 Biotech 85 Trace Analysis 21 GC/SH 85 Generic 86 — Laboratory Basics Routine Use 23 Histology 87 Acids & Bases 23 HPLC 87 Generic 26 NMR 87 Inorganic Reagents 27 Pharmacopoeia 90 Organic Reagents 32 Returnable Containers 91 Spectroscopy 92 Laboratory Basics—Special Use 36 Acids & Bases 36 Titration 94 Generic Reagents 36 Buffers 94 Inorganic Reagents 38 Indicators 95 Organic Reagents 51 Volumetric Solutions 96 LPLC/TLC/Paper Chromatography 58 Flash Chromatography 58 LPLC 60 Paper 67 TLC 68 NOTE: This publication is designed for electronic use only. Hazard and Safety information can be found on product detail pages and at sigma-aldrich.com/safetycenter. 2 General Laboratory Reagents Technical Support: [email protected] Analytical—Other Derivatisation Name Cat. No. Bis(2-ethylhexyl) phthalate, Selectophore™ 80030-1ML Derivatisation 80030-5ML Name Cat. No. 80030-25ML Aerosol® 22 A9753-100ML Bis(2-ethylhexyl) sebacate, Selectophore™, ≥97.0% 84818-5ML A9753-500ML 84818-25ML Aluminum chloride - ethanol solution, ~11% in ethanol/water, for TLC 28672 N,N′-Bis(salicylidene)-o-phenylenediamine Vanadium(IV) oxide Complex, 68541-25MG-F derivatization ≥98.0% (HPLC) 68541-100MG-F Antimony(III) chloride reagent (free of CHC), for thin layer chromatography, 21623-100ML Bis(triphenylphosphoranylidene)ammonium chloride, Selectophore™ 15263-1G-F SbCl3 29-31 % Bisulfite ionophore, Selectophore™ 95647-50MG-F Bispyrazolone, for TLC derivatization, for the det.of cyanide, ≥98.0% 15156-5G Buffer solution for optode membranes pH 5.5, for optode membranes, 0.16 M 82629-100ML Bromocresol Green solution, indicator, ready-to-use 02559-500ML sodium acetate Bromothymol Blue, pkg of 200 mL 34656 Buffer solution for optode membranes pH 7.6, for optode membranes 82626-100ML Curcumin solution, ~0.1 % (w/v) (in ethanol with 2M HCl (99:1 v/v)), for TLC 28982-100ML tert-Butylcalix[4]arene-tetrakis(N,N-dimethylacetamide), Selectophore™, 19734-250MG derivatization ≥97.0% 2′,7′-Dichlorofluorescein solution, indicator, ready-to-use 02591-500ML Cadmium ionophore I, Selectophore™ 20909-50MG-F +2 2,6-Dichloroindophenol sodium salt hydrate, ≥97.0% (calc. based on dry 36180-1G-F Cadmium ion standard solution, 0.1 M Cd , analytical standard, for ion- 20893-250ML-F substance, AT) 36180-5G-F selective electrodes 36180-25G-F Calcium ionophore I, Selectophore™, function tested, ≥99.0% 21192-50MG-F 4-(Dimethylamino)benzaldehyde, for the determination of hydroxyproline, 39070-50G 21192-250MG-F ≥99.0% (HPLC) 39070-250G Calcium ionophore II, Selectophore™, function tested 21193-50MG-F 4-(Dimethylamino)benzaldehyde solution, 10 g/L in isopropanol, for TLC 02560-500ML 21193-250MG-F derivatization Calcium ionophore II - cocktail B, Selectophore™ 21199 5-(4-Dimethylaminobenzylidene)rhodanine, for TLC derivatization, ≥98.0% 39090-10G Calcium ionophore III, Selectophore™, function tested 21186-5MG-F 2,4-Dinitrophenylhydrazine hydrochloric acid solution, ~0.005 M in ethanol, for 18189-100ML-F 21186-25MG-F thin layer chromatography Calcium ionophore IV, Selectophore™, function tested 21198-50MG-F 2,4-Dinitrophenylhydrazine Phosphoric acid solution, for derivatization (of 42215-250ML 21198-250MG-F aldehydes and ketones), substitute for the explosive solid, ~0.2 M (~4%) Calcium ionophore V, Selectophore™, ≥95% 21203-25MG-F Dragendorff reagent, for TLC derivatization 44578-100ML-F 21203-250MG-F Fluorescamine, pkg of 100 mL 34653 Calcium ionophore VI, Selectophore™, function tested 72385-50MG-F Ninhydrin reagent according to Stahl, for thin layer chromatography 17975-100ML 72385-250MG-F Calcium ionophore I - cocktail A, Selectophore™ 4-(4-Nitrobenzyl)pyridine, for TLC derivatization, for spectrophotometric det. of 73210-5G 99310-0.1ML-F phosphorus-containing pesticides 73210-25G Calcium ionophore II - cocktail A 99371-0.1ML-F 73210-250G Calcium Ionophore IV - Cocktail for membranes, Selectophore™ 70356-1EA-F Phospray, pkg of 200 mL 33047-U 70356-1ML-F Rhodamine B solution, 0.2% in isopropanol, for TLC derivatization 02558-100ML Calcium ion solution for ISE, 0.1 M Ca, analytical standard (for ion-selective 21059-100ML-F 02558-500ML electrodes) 21059-250ML-F Carbonate ionophore IV solution, in THF (50 mg/0.5 mL), Selectophore™ 21856-1EA Carbonate ionophore VII, Selectophore™, function tested 93206-50MG 93206-500MG Cefixime trihydrate, for microbiology, ≥98.0% (HPLC) 18588-25MG-F Electrochemistry 18588-100MG-F Name Cat. No. Cellulose triacetate, Selectophore™ 22199-50G-F Cerium(III) Ionophore, Selectophore™, function tested Activity standard solution for fluoride electrode (100 ppm as fluoride) 443174-475ML 44967-50MG-F 44967-250MG-F Adapter F-BNC 11509-1EA Cesium ionophore I, Selectophore™, function tested 21007-50MG Aluminum Ionophore I, Selectophore™, function tested 55593-50MG Cesium ionophore II, Selectophore™, function tested, ≥97.0% 83757-50MG-F Amine ionophore I, Selectophore™, function tested 06571-50MG Cesium Ionophore III, Selectophore™, function tested 01657-50MG Ammonium ionophore I, Selectophore™, function tested, ≥95.0% (Nonactin, 09877-50MG Cetyl alcohol, Selectophore™, ≥99.0% HPLC) 09877-250MG 52238-1G Ammonium ionophore I - cocktail A, Selectophore™ 52238-5G 99978-0.1ML-F 52238-25G Ammonium ionophore I - cocktail B, Selectophore™ 09882-0.1ML Chloride ionophore I, Selectophore™ 24897-50MG + Ammonium ion solution for ISE, 0.1 M NH4 , analytical standard (for ion- 09683-100ML Chloride ionophore I - cocktail A, Selectophore™ selective electrodes) 99408-0.1ML-F Chloride ionophore II, Selectophore™, function tested 24901-25MG Ammonium ion solution for ISE, 1000 mg/kg N, analytical standard (for ion- 09685-250ML selective electrodes) Chloride ionophore III, Selectophore™ 24894-50MG Chloride ionophore IV, Selectophore™ Ammonium nitrate solution, 0.9-1.1 M, for Ca-selective electrodes 44277-100ML 92332-25MG-F 92332-100MG-F Arsenite Ionophore I, Selectophore™, function tested 30236-50MG Chloride-selective liquid ion-exchanger microelectrode cocktail A, Selectophore™ 98183-0.1ML-F Benzyl acetate, Selectophore™, ≥99.5% 43957-1ML-F Chloroparaffin, Selectophore™, 60% chlorine basis 25720-5ML-F Benzyl 2-nitrophenyl ether, Selectophore™, ≥99.0% 13610-5ML-F Chromium(III) Ionophore III, Selectophore™, function tested 41433-50MG Bis(1-butylpentyl) adipate, Selectophore™, ≥98.0% 02150-5ML Chromoionophore I, Selectophore™ 02150-25ML 27086-10MG-F 27086-100MG-F Bis(1-butylpentyl)decane-1,10-diyl diglutarate, Selectophore™ 30585-1G Chromoionophore II, Selectophore™ 27087-10MG-F Bis(2-ethylhexyl) adipate, 99% 525197-5ML Chromoionophore III, Selectophore™ 525197-1L 27088-10MG-F Chromoionophore IV, Selectophore™ Bis(2-ethylhexyl) adipate, Selectophore™, ≥99.0% 02138-1ML 27089-10MG-F 02138-5ML Chromoionophore VI, Selectophore™ 27095-100MG-F 02138-25ML For further information, visit sigma-aldrich.com General Laboratory Reagents 3 Analytical—Other Electrochemistry Name Cat. No. Name Cat. No. Chromoionophore VII, Selectophore™ 27097-10MG-F Ethylenediaminetetraacetic acid diammonium copper salt solution, volumetric, 46122-250ML-F 27097-100MG-F 0.025 M EDTA-Cu(NH4)2 46122-1L-F Chromoionophore VIII, Selectophore™ 27098-50MG-F [12-(4-Ethylphenyl)dodecyl] 2-nitrophenyl ether, Selectophore™, ≥95.0% 46092-250MG-F Chromoionophore IX, Selectophore™ 28218-50MG Fluoride ionophore I, Selectophore™ 02536-100MG-F Chromoionophore X, Selectophore™ 28222-10MG Fluoride ion solution for ISE, 0.1 M F-, analytical standard (for ion-selective 47072-100ML electrodes) Chromoionophore XI, Selectophore™ 27102-50MG-F 2-Fluorophenyl 2-nitrophenyl ether, Selectophore™, ≥98.0% Chromoionophore XVII, Selectophore™ 19684-100MG-F 47390-5ML-F Glass Plate, Selectophore™, plan Cobalt(II) ionophore IV, Selectophore™, function tested 11491-50MG 48952-1EA-F Glass Ring, Selectophore™, borosilicate glass; face ground on both sides Copper(II) ionophore I, Selectophore™ 61193-50MG 48954-1EA Glass Ring, Selectophore™, borosilicate glass; face ground on both sides Copper(II) Ionophore V, Selectophore™, function tested 38788-50MG 48953-1EA-F 38788-250MG hemi-Calcium bis(2-ethylhexyl)phosphate, Selectophore™ 08733-1G Copper(II) Ionophore IV, Selectophore™, function tested 50242-10MG-F hemi-Calcium bis[4-(1,1,3,3-tetramethylbutyl)phenyl] phosphate, Selecto- 15180-1G 50242-100MG-F phore™, ≥97.0% Cyanide Ionophore II, Selectophore™, function tested 40585-50MG-F Hydrogen ionophore I, Selectophore™, function tested 95292-100MG Cyclohexanone, Selectophore™, ≥99.5% 29135-10ML 95292-500MG 29135-100ML Hydrogen ionophore II, Selectophore™ 95295-50MG 29135-500ML Hydrogen ionophore III, Selectophore™, ≥99.0% 95298-50MG 1-Decanol, Selectophore™, ≥98.0% 30608-5ML Hydrogen ionophore IV, Selectophore™, function tested 95296-50MG 30608-25ML Hydrogen ionophore V, Selectophore™, function tested 16979-50MG Dibutyl butylphosphonate, Selectophore™, ≥99.0% 38479-5ML-F Hydrogen ionophore I - cocktail A, Selectophore™ 95291-0.1ML Dibutyl phthalate, Selectophore™ 80100-5ML Hydrogen ionophore I - cocktail B, Selectophore™ 80100-25ML 95293-0.1ML Hydrogen sulfite ionophore I, Selectophore™ Dibutyl sebacate, Selectophore™, ≥97.0% 84838-5ML 54110-250MG 84838-25ML Hydrogen sulfite ionophore II, Selectophore™, function