Product datasheet [email protected] ARG23568 Package: 50 μg anti-NCR1 antibody [29A1.4] (FITC) Store at: 4°C Summary Product Description FITC-conjugated Rat Monoclonal antibody [29A1.4] recognizes NCR1. Rat anti Mouse CD335 antibody, clone 29A1.4 recognizes natural killer cell p46-related protein (NKp46), otherwise known as CD335, which is uniquely expressed by resting and activated natural killer (NK) cells, but no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes. It is a major NK cell lysis receptor for autologous pathogen-infected and tumor target cells during natural cytotoxicity responses. Ligands of CD335 include viral hemagglutinins (HAs) and heparan sulfate proteoglycans (HSPGs) on the surface of tumor cells. Staining with Rat anti mouse CD335 (29A1.4) is not strain specific and the antibody has been used to stain C57BL/6, SJL, CBA/CA and BALB/c strains. Clone 29A1.4 also activates NK cells in vitro. It does not deplete NK cells in vivo. Tested Reactivity Ms Tested Application FACS Host Rat Clonality Monoclonal Clone 29A1.4 Isotype IgG2a Target Name NCR1 Antigen Species Mouse Immunogen NKP46-IgG1 Fc fusion protein. Conjugation FITC Alternate Names CD antigen CD335; Natural killer cell p46-related protein; Lymphocyte antigen 94 homolog; Natural cytotoxicity triggering receptor 1; hNKp46; NKP46; NKp46; NK cell-activating receptor; LY94; CD335; NK- p46 Application Instructions Application table Application Dilution FACS 1:2 - 1:5 Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 34 kDa Properties Form Liquid Purification Purified Buffer PBS and 0.09% Sodium azide. www.arigobio.com 1/2 Preservative 0.09% Sodium azide Concentration 0.5 mg/ml Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol NCR1 Gene Full Name natural cytotoxicity triggering receptor 1 Function Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. [UniProt] www.arigobio.com 2/2 Powered by TCPDF (www.tcpdf.org).