US009096909B2 (12) United States Patent (10) Patent No.: US 9,096,909 B2 Preuss et al. (45) Date of Patent: Aug. 4, 2015 (54) SORGHUM CENTROMERE SEQUENCES AND 5,733,744 A 3, 1998 Hamilton MNCHROMOSOMES 5,766,885 A 6/1998 Carrington et al. 5,773,705 A 6/1998 Vierstra et al. 5,866,793 A 2/1999 Baga et al. (75) Inventors: Daphne Preuss, Chicago, IL (US); 5,869,294 A 2/1999 Harrington et al. Pierluigi Barone, Charleston, IL (US); 5,877.402 A 3/1999 Maliga et al. Shawn R. Carlson, Bondville, IL (US); 5,891,625 A 4/1999 Buchardt et al. Gregory P. Copenhaver, Chapel Hill, 5,891,691 A 4/1999 Hadlaczky et al. 5,925,808 A 7, 1999 Oliver et al. NC (US); Song Luo, Chicago, IL (US); 5,977.439 A 11/1999 Hamilton Jennifer M. Mach, Chicago, IL (US) 5,977,441 A 11/1999 Oliver et al. 6,025,155 A 2/2000 Hadlaczky et al. (73) Assignee: CHROMATIN, INC., Chicago, IL (US) 6,048,692 A 4/2000 Maracas et al. 6,077,697 A 6/2000 Hadlaczky et al. 6,127,171 A 10/2000 Slilaty et al. 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Date: Jan. 27, 2011 FOREIGN PATENT DOCUMENTS (65) Prior Publication Data EP O 320 500 A2 6, 1989 EP O 338 266 A2 10, 1989 US 2012/O255O72A1 Oct. 4, 2012 (Continued) OTHER PUBLICATIONS Related U.S. Application Data Bedell et al., Sorghum genome sequencing by methylation filtration, (60) Provisional application No. 61/228,015, filed on Jul. PLOS Biol. 3:104-15 (2005). 23, 2009. Carlson et al., Meiotic transmission of an in vitro-assembled autono mous maise minichromosome, PLOS Gen., 3:1965-74 (2007). Miller et al., Retrotransposon-related DNA sequences in the (51) Int. Cl. centromeres of grass chromosomes, Genetics, 150: 1615-23 (1998). CI2N 15/82 (2006.01) Zwicket al., Distribution and sequence analysis of the centromere CI2N 15/63 (2006.01) associated repetitive element CEN38 of Sorghum bicolor (Poaceae), CI2N 15/09 (2006.01) Am. J. Botany, 87: 1757-64 (2000). AOIH 5/00 (2006.01) Extended European Search Report, European Patent Office, CI2O I/68 (2006.01) EP10802957, dated Aug. 21, 2013. Abdullah et al., Efficient plant regeneration from rice protoplasts (52) U.S. Cl. through somatic embryogenesis, BioTechnology, 4: 1087-90 (1986). CPC .......... CI2O 1/6895 (2013.01); C12N 15/8245 Abel et al., Delay of disease development in transgenic plants that (2013.01) express the tobacco mosaic virus coat protein Gene, Science, 232: (58) Field of Classification Search 738-43 (1986). CPC ... C12N 15/09; C12N 15/82: C12N 15/8245; C12Q 1/6895 (Continued) See application file for complete search history. Primary Examiner — Brent T Page (74) Attorney, Agent, or Firm — Marshall, Gerstein & Borun (56) References Cited LLP U.S. PATENT DOCUMENTS (57) ABSTRACT 4,889,806 A 12/1989 Olson et al. The invention is generally related to MCs containing Sor 5,270,201 A 12/1993 Richards et al. ghum centromere sequences. 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