CONTCENTER FOR GENOMIC REGUL CRG SCIENTIFIC STRUCTURE . 4 CRG MANAGEMENT STRUCTURE . 6 CRG SCIENTIFIC ADVISORY BOARD (SAB) . 8 CRG BUSINESS BOARD . 9 YEAR RETROSPECT BY THE DIRECTOR OF THE CRG: MIGUEL BEATO . 10 GENE REGULATION. 14 p Chromatin and gene expression .....................16 p Transcriptional regulation and chromatin remodelling .....19 p Regulation of alternative pre-mRNA splicing during cell . 22 differentiation, development and disease p RNA interference and chromatin regulation . 26 p RNA-protein interactions and regulation . 30 p Regulation of protein synthesis in eukaryotes . 33 p Translational control of gene expression . 36 DIFFERENTIATION AND CANCER ...........................40 p Hematopoietic differentiation and stem cell biology..........42 p Myogenesis.....................................46 p Epigenetics events in cancer.......................49 p Epithelial homeostasis and cancer ...................52 ENTSATION ANNUAL REPORT 2006 GENES AND DISEASE .................................56 p Genetic causes of disease .............................58 p Gene therapy ......................................63 p Murine models of disease .............................66 p Neurobehavioral phenotyping of mouse models of disease .....68 p Gene function ......................................73 p Associated Core Facility: Genotyping Unit..................76 BIOINFORMATICS AND GENOMICS ..........................80 p Bioinformatics and genomics ...........................82 p Genomic analysis of development and disease ..............86 p Associated Core Facility: Microarrays Unit.................88 CELL AND DEVELOPMENTAL BIOLOGY ........................90 p Microtubule function and cell division ....................92 p Sensory cell biology and organogenesis ..................96 SYSTEMS BIOLOGY ..................................100 p Design of biological systems..........................102 p Systems analysis of development .......................107 p Gene Network Engineering ............................111 p Metazoan Systems ..................................115 APPENDIX 1: ...........................................120 p V CRG Annual Symposium: “Systems Biology: A Cell in the Computer?” APPENDIX 2: ...........................................122 p CRG Seminars and Programme Seminars APPENDIX 3:............................................129 p Grants CRG SCIENTIFIC STRUCTURE BOARD OF TRUSTEES DIRECTOR Miguel Beato DEPUTY DIRECTOR Luis Serrano SCIENTIFIC SCIENTIFIC FACULTY BOARD EXECUTIVE BOARD BUSINESS BOARD ADVISORY BOARD COMMITTEE GENE BIONFORMATICS CELL AND SYSTEMS DIFFERENTIATION GENES REGULATION AND GENOMICS DEV. BIOLOGY BIOLOGY AND CANCER AND DISEASE MIGUEL BEATO RODERIC GUIGÓ ISABELLE VERNOS LUIS SERRANO THOMAS GRAF XAVIER ESTIVILL Coordinator Coordinator Acting Coordinator Coordinator Coordinator Coordinator MICROTUBULES CHROMATIN AND BIOINFORMATICS AND DESING OF HEMATOPOIETIC GENETIC CAUSES FUNCTION AND CELL GENE EXPRESSION GENOMICS BIOLOGICAL SYSTEMS DIFFERENTIATION AND OF DISEASE DIVISION RODERIC GUIGÓ STEM CELL BIOLOGY XAVIER ESTIVILL MIGUEL BEATO ISABELLE VERNOS LUIS SERRANO Group Leader THOMAS GRAF Group Leader Group Leader Group Leader Group Leader ............................... Group Leader TRANSCRIP. REGULATION FUNCTIONAL GENOMICS AND CHROMATIN OF NEUROLOGICAL DISORDERS REMODELLING GENOMIC ANALYSIS SENSORY CELL BIOLOGY SYSTEMS ANALYSIS EULÀLIA MARTÍ ALBERT JORDAN OF DEVELOPMENT AND AND ORGANOGENESIS OF DEVELOPMENT MYOGENESIS Staff Scientist Staff Scientist DISEASE HERNAN LOPEZ-SCHIER JAMES SHARPE PURA MUÑOZ LAURO SUMOY Group Leader Group Leader Group Leader Group Leader REGULATION OF ALTERNATIVE GENE THERAPY PRE-MRNA SPLICING CRISTINA FILLAT CORE FACILITY- JUAN VALCÁRCEL CORE FACILITY- GENE NETWORK EPIGENETICS EVENTS Group Leader MICROARRAYS UNIT Group Leader ADVANCED LIGHT ENGINEERING IN CANCER LAURO SUMOY MICROSCOPY UNIT MARK ISALAN LUCIANO DI CROCE Unit Leader (Under Construction) Group Leader Group Leader RNA INTERFERENCE AND MURINE MODELS CHROMATIN REGULATION OF DISEASE RAMIN SHIEKHATTAR EPITHELIAL HOMEOSTA- MARIONA ARBONÉS METAZOAN SYSTEMS Group Leader SIS AND CANCER Group Leader BEN LEHNER SALVADOR AZNAR-BENITAH Group Leader Group Leader TRANSLATIONAL CONTROL NEUROBEHAVIORAL PHENOTYPING OF OF GENE EXPRESSION MOUSE MODELS OF RAÚL MÉNDEZ DISEASE Group Leader CORE FACILITY- MARA DIERSSEN FACS UNIT Group Leader REGULATION OF PROTEIN SYNTHESIS GENE FUNCTION IN EUKARYOTES SUSANA DE LA LUNA FÁTIMA GEBAUER Group Leader Group Leader RNA-PROTEIN INTERAC- CORE FACILITY- TIONS AND REGULATION GENOTYPING UNIT JOSEP VILARDELL MÓNICA BAYÉS Group Leader Unit Responsible CORE FACILITY- PROTEOMICS UNIT (Under Construction) CRG MANAGEMENT STRUCTURE BOARD OF TRUSTEES DIRECTOR CEO MIGUEL BEATO MARIAN MARRODAN ASSISTANT ASSISTANT MONTSERRAT RODÓN CRISTINA CASAUS RESEARCH ORGANIZATION HUMAN GENERAL COMMUNICATION OFFICE, FINANCES TECHNOLOGY AND ICT RESOURCES / SERVICES AND PUBLIC AND GRANTS SCIENTIFIC TRANSFER MANAGEMENT LEGAL DEPARTMENT MANAGEMENT RELATIONS OFFICERS MANAGEMENT SILVIA TÓRTOLA EVA DEL PINTO ISABEL BELLOT CRISTINA CASAUS JOSEP QUERALT GLORIA LLIGADAS PROGRAMME HHRR-PERSONNEL PURCHASING AND RESEARCH OFFICE SECRETARIES AND LOGISTICS GENETIC CAUSES CLARA LÓPEZ MAGALÍ BARTOMEUS COLLABORATORS SERGI REPULLO OF DISEASE MANAGEMENT MARIBEL CORRAL EVA IBÁNEZ MIREIA PÉREZ ÀUREA RODRÍGUEZ GLORIA FREIXAS GEMMA PÉREZ MONTSERRAT ESTAPÉ IMMA FALERO VERONICA JAREÑO BLANKA WYSOCKA NICOLA COLLU DESIGN OF ACCOUNTING HHRR-FELLOWSHIP BIOLOGICAL AND TECHNICAL OWNERS AND SYSTEMS SCIENTIFIC STUDENTS MANAGEMENT MICHELA BERTERO SERVICES RECEPTION / ENRIC GUMMÀ ADMINISTRATIVE MARIBEL DAVID MAINTENANCE SUPPORT MARÍA ROZAS JOSEP QUERALT SONIA PARAREDA JAUME BACARDIT SASCHA ANDREU BÁRBARA FERREIRA HHRR-SUPPORT ESTHER SALAS CONTROL AND WORKING RISKS / PROJECTS RADIOACTIVITY / MANAGEMENT JUSTIFICATON WASTES MARTA MOLINA SYSTEMS Outsourcing SONIA ALCÁZAR ÓSCAR FRAILE MªJOSÉ ROMA JAUME BACARDIT LEGAL ENRIC GUMMÀ DEPARTMENT EVA DEL PINTO CRISTINA CASAUS SYSTEMS ADMINISTRATION EiPMC AND SUPPORT SERVICE ARANTXA VENTURA Outsourcing CRG SCIENTIFIC ADVISORY BOARD (SAB) DR. KAI SIMONS (chairman) Max Planck Institute of Molecular Cell Biology and Genetics, Dresden DR. STYLIANOS EMMANUEL ANTONARAKIS Medical Genetics, University of Geneva, Geneva DR. MICHAEL ASHBURNER European Bioinformatics Institute-EMBL Outstation, Department of Genetics, University of Cambridge, Cambridge DR. PIERRE CHAMBON Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Strasbourg DR. IAIN MATTAJ European Molecular Biology Laboratory (EMBL), Heidelberg DR. JOAN MODOLELL Center of Molecular Biology “Severo Ochoa”, CSIC & Autonomous University of Madrid, Madrid DR. ARNOLD MUNNICH Det. Génétique, Hôpital des Enfants Malades, Paris DR. CHRISTIANE NÜSSLEIN-VOLHARD Abt. Genetik, Max Planck Institut für Entwicklungsbiologie, Tübingen DR. MARC VIDAL Dana Farber Cancer Institute, Boston, USA DR. ERWIN WAGNER Research Institute of Molecular Pathology (IMP), Vienna CRG BUSINESS BOARD PRESIDENT DR. ANTONI ESTEVE President, Esteve VICE-PRESIDENTS DR. JOSEP PROUS, JR. Executive Vice President, Prous Science SR. FERNANDO TURRÓ General Manager, Contratas y Obras MEMBERS SR. JESÚS ACEBILLO President, Novartis Farmacéutica, S.A. SR. RAFAEL BENJUMEA CABEZA DE VACA General Manager, Fundación Marcelino Botín DR. PERE BERGA R+D Management Director, Almirall Prodesfarma, S.A. SR. ANTONI GELONCH Corporate Social Responsibility Director, Sanofi-Aventis, S.A. and General Manager of the Aventis Foundation DR. GONZALO HERNÁNDEZ HERRERO Medical Director, Pfizer España, S.A. SR. RAFAEL PARDO AVELLANEDA General Manager, Fundación BBVA SR. JORDI RAMENTOL General Manager, Grupo Ferrer Internacional, S.A. SR. NARCÍS SERRA President, Caixa Catalunya (Grupo) YEAR RETROSPECT by the Director of CRG: Miguel Beato The past year has been dominated by the moving to the new building of the PRBB. Although new groups started to work in the new building during the summer, the majority of the new and established groups moved betwe- en October and December. By the end of the year only the Genotyping Facility and the Microarray Unit were still in the CMIMA building, waiting for the installation of appropriate spaces in the PRBB building. Another important event was the official signature of the partnership agreement with the European Molecular Biology Laboratory (EMBL), for the creation of an EMBL-CRG Research Unit in Systems Biology. The presenta- tion to the media took place on September 7. The agreement foresees financing of four independent research groups for a period of 9 years sub- jected to periodic evaluations. The Unit will be directed by Luis Serrano, the coordinator of the CRG Systems Biology programme. These important events took place in the context of a crisis in the coali- tion of political parties in power that lead to the outing from the Catalan Government of the Minister in charge of Research. Until the foreseen elec- tions in November, a provisional government was named with changes in the research competences. Under these conditions it was not possible to secure a finance planning for the next years despite a personal engage- ment of the President of the Generalitat. Nevertheless we have managed to go on with our roadmap and, after long rounds of interviews, we have recruited six new group leaders and a unit leader, five of them foreigners. The increasing number of groups and units has imposed a new organiza- tion of the leading structure. The former Direction Committee has split