ISSN 1814-2206 International Hop Growers` Convention I.H.G.C. Proceedings of the Scientific Commission INTERNATIONAL HOP GROWERS`CONVENTION Tettnang, Germany 24 - 28 June 2007 Impressum: Scientific Commission International Hop Growers` Convention (I.H.G.C.) Hop Research Center Hüll, Hüll 5 1/3, 85283 Wolnzach, Germany Internet:: http://www.lfl.bayern.de/ipz/hopfen/10585/index.php Dr. Elisabeth Seigner June 2007 © Scientific Commission, I.H.G.C. 2 With Special Thanks to our Sponsors (logos arranged randomly) Tettnanger Hopfen Weltweit 3 Contents Page Foreword ............................................................................................................................ 7 Vorwort............................................................................................................................... 8 Lectures and Posters....................................................................................................... 9 I. Session: HOP BREEDING............................................................................................. 9 The UK Hop Breeding Programme: A new Site and new Objectives P. Darby........................................................................................................................... 10 Aspects of breeding aroma hops V. Nesvadba, K. Krofta................................................................................................... 14 Breeding and development of triploid aroma cultivars R. Beatson, P. Alspach .................................................................................................. 18 Molecular marker development and use in the New Zealand hop breeding programme E. Buck, C. Wiedow, R.A. Beatson................................................................................ 22 Selection methods in hop disease resistance breeding in Slovenia S. Radišek, A. Čerenak, B. Javornik ............................................................................. 26 Evaluation of some wild hops from Europe G. Probasco, C. Ocamb, S. Varnum, N. Miller.............................................................. 30 Wild hops – new sources for resistance to powdery mildew A. Lutz, J. Kneidl, E.Seigner.......................................................................................... 31 Variability of wild hops (Humulus lupulus L.) in Czech Republic V. Nesvadba, K. Krofta, J. Patzak ................................................................................. 32 Wild Hops from Northern Parts of Caucasus V. Nesvadba.................................................................................................................... 33 Cryoconservation of tissue cultures P. Svoboda, M. Faltus .................................................................................................... 34 II. Session: DEVELOPMENTAL PHYSIOLOGY ............................................................ 35 Phytohormones and phase change in hops M.A. Revilla, N. F. Villacorta, J.A. Magadán, H. Fernández ........................................ 36 III. Session: HOP CHEMISTRY ..................................................................................... 40 Commercial hop extracts rich in Xanthohumol M. Biendl ......................................................................................................................... 41 Changes in antioxidant properties of hops in the course of drying, pelletizing and storage. K. Krofta, A. Mikyška, D. Hašková ............................................................................... 45 Presenting, evaluating and comparing chemical composition of hop secondary metabolites at a glance M. Kač, R. Hrastar, I. J. Košir ........................................................................................ 49 The biosynthesis of the bitter acids in hops K. Kammhuber, M. Goese, S. Hecht ............................................................................. 53 4 Low molecular polyphenols in beer influenced by different hop varieties R. Schmidt, B. Beck ....................................................................................................... 54 IV. Session: DNA-BASED TECHNIQUES IN HOP RESEARCH ................................... 55 Molecular markers for alpha acids; study for practical application Y. Okada, K. Koie, A. Inaba, T. Kaneko and K. Ito ........................................................................................... 56 QTL Mapping of alpha acid content in hop A. Cerenak., Z. Satovic.., J. Jakše, Z. Luthar, K. Carovic-Stanko and B. Javornik ... 60 Molecular methods in resistance hop research J. Patzak.......................................................................................................................... 63 cDNA-AFLP makers for powdery mildew resistance in hops (Humulus lupulus L.) R. Seidenberger, S. Mikolajewski, A. Lutz, E. Seigner, S. Seefelder, W.E. Weber.... 67 Genome analysis – an important tool to support classical hop breeding , S. Seefelder, R. Seidenberger, A. Lutz, E. Seigner...................................................... 71 Towards the genetic map for Verticillium wilt resistance J. Jakše, Z. Luthar, A. Čerenak, S. Radišek, B. Javornik............................................ 72 Use of Diversity Array Technology (DArT) for genotyping Humulus lupulus L. S. Whittock, J. Jakse, J. Carling, P. Matthews, G. Probasco, J. Henning, P. Darby, A. Cerenak, B. Javornik, A. Kilian, G. Leggett, A. Koutoulis...................................... 73 Constitutive Expression of a Grapevine Stilbene Synthase Gene in Transgenic Hop (Humulus lupulus L.) Yields Resveratrol and its Derivatives in Substantial Quantities G. Weber, M. Höhnle, N. Pitsch, K.R. Häntzschel, S. Stahringer, O. Spring, B. Pickel, A. Heyerick, D. de Keukeleire....................................................................... 74 Production of powdery mildew resistant hops via gene transfer H. Miehle, E. Seigner...................................................................................................... 78 Agrobacterium-mediated transformation of hop S. Škof, Z. Luthar............................................................................................................ 82 Some aspects of regulation hop chalcone synthase CHS_H1 in heterologous systems, cloning and analysis of hop regulatory factors J. Matoušek, J. Škopek, J. Patzak, T. Kocábek, L. Maloukh, J. De Keukeleire, A. Heyerick, Z. Fussy, I. Roldán-Ruiz, D. De Keukeleire............................................. 83 Molecular Analysis of Hop Chalcone Synthases J. Škopek, A. Heyerick, T. Kocábek, J. Matoušek ....................................................... 87 Optimalisation of a Real Time RT-PCR protocol for the analysis of gene expression in hop tissues L. Maloukh, J. De Keukeleire, E. Van Bockstaele, I. Roldán-Ruiz.............................. 88 The propagation of HLVd in developing hop pollen and its elimination upon expression of apoptotic nuclease J. Matoušek, K. Pešina, J. Škopek, L. Orctová ............................................................ 89 V. Session: HOP DISEASES AND PESTS .................................................................... 90 Hop Flea Beetle (Psylliodes attenuatus Koch) in Slovenia M. Rak Cizej, L. Milevoj.................................................................................................. 91 Study on cause of Fusarium cone tip blight E. Solarska...................................................................................................................... 95 Proteomic analysis of the fungus Verticillium albo-atrum S. Mandelc, S. Radišek, P. Jamnik, B. Javornik .......................................................... 99 5 Population dynamics of Phorodon humuli (Schrank, 1801) (Hemiptera: Aphididae) in hop cones A. Lorenzana, A. Hermoso de Mendoza, J.A. Magadán, M.V. Seco ......................... 100 Resistance to imidacloprid (Confidor 70 WG) in damson-hop aphid (Phorodon humuli Schrank) on Czech hops J. Vostrel ....................................................................................................................... 101 An ancient compound rediscovered: perspectives of aphid control in organic hop growing by the use of quassia products F. Weihrauch, M. Eckert, B. Engelhard....................................................................... 105 Contents of pesticide residues in hops K. Krofta, J. Vostřel ...................................................................................................... 109 Standard ranges of the application of pesticides in hops – a proposal for the EPPO guide lines B. Engelhard, J. Schwarz, F. Weihrauch .................................................................... 110 Molecular Variability within the Coat Protein Gene of Hop Mosaic and Hop Latent Carlaviruses D. Crowle., S. Pethybridge., C.Wilson......................................................................... 114 Nematodes Associated with Hop Production in Tasmania, Australia S. Pethybridge, F. Hay ................................................................................................. 115 The Compendium of Hop Diseases, Arthropod Pests and Other Diseases S. Pethybridge., W. Mahaffee., D. Gent ....................................................................... 116 VI. Session: HOP PRODUCTION ................................................................................. 117 Influence of weather conditions and irrigation on yield and quality of hops J. Kopecký, J. Ježek....................................................................................................