GS Junior Instrument Owner’s Manual June 2012 Instrument Kit GS Junior Junior GS FLX+ XL+ GS FLX+ XLR70 GS FLX XLR70 For life science research only. Not for use in diagnostic procedures. GS Junior Instrument Owner’s Manual GS Junior Instrument Owner’s Manual 1 Preface .................................................................................................................................. 5 1.1 About this Manual ...................................................................................................................................... 5 1.2 GS Junior System Documentation Set ................................................................................................... 5 1.3 Revision History .......................................................................................................................................... 8 1.4 Icons .............................................................................................................................................................. 9 1.5 Assistance .................................................................................................................................................... 9 1.6 Disclaimers ................................................................................................................................................ 11 1.6.1 Warranty ............................................................................................................................................................................... 11 1.6.2 Intended Use of the GS Junior Instrument ............................................................................................................. 11 1.6.3 Notice to Purchaser ......................................................................................................................................................... 11 1.6.4 Open Source Software .................................................................................................................................................... 11 1.6.5 Trademarks .......................................................................................................................................................................... 12 1.6.6 License Disclaimers ......................................................................................................................................................... 12 1.6.7 Declaration of Conformity ............................................................................................................................................. 12 1.6.8 Safety ..................................................................................................................................................................................... 13 1.6.8.1 Normal Operation ................................................................................................................................................. 13 1.6.8.2 Specific Warnings ................................................................................................................................................. 13 1.6.8.3 Specific Cautions .................................................................................................................................................. 14 1.7 Specifications of the GS Junior System ............................................................................................... 15 1.8 GS Junior System Site Requirements .................................................................................................. 16 1.9 Disposal of the Instrument ..................................................................................................................... 17 2 The GS Junior System ...................................................................................................... 18 2.1 Hardware Components ........................................................................................................................... 18 2.1.1 Main Components of the GS Junior Instrument .................................................................................................. 18 2.1.2 Attendant PC....................................................................................................................................................................... 21 2.1.3 Separate Computer Resource for Data Analysis (DataRig) ............................................................................ 21 2.1.4 Accessories ......................................................................................................................................................................... 21 2.2 Software Components ............................................................................................................................. 22 2.3 Components Not Supplied...................................................................................................................... 24 2.4 GS Junior System Installation ................................................................................................................ 24 3 Turning the GS Junior System On and Off, Idle Time, and Storage ....................... 25 3.1 Turning On the GS Junior System ......................................................................................................... 25 3.2 Turning Off the GS Junior System ......................................................................................................... 26 3.3 Leaving the GS Junior Instrument Idle or Preparing It for Storage ............................................... 27 3.3.1 Idle Time ............................................................................................................................................................................... 27 3.3.2 Preparing the GS Junior Instrument for Storage ................................................................................................. 28 3.3.3 Reactivating the GS Junior Instrument After Storage ....................................................................................... 31 4 Overview of a Sequencing Run in the GS Junior System ......................................... 32 4.1 Continuous Instrument Operation ........................................................................................................ 32 4.2 Before You Begin a Sequencing Run ................................................................................................... 32 4.2.1 State of the Instrument ................................................................................................................................................... 32 June 2012 3 GS Junior Instrument Owner’s Manual 4.2.2 What You Should Already Have ................................................................................................................................. 33 4.3 Initiating and Monitoring a Sequencing Run ..................................................................................... 34 5 Overview of Data Processing in the GS Junior System ............................................ 37 6 GS Junior Instrument Maintenance .............................................................................. 39 6.1 Sipper Tubes, Filters, and Buffer Tray Replacement ......................................................................... 39 6.1.1 Removing Used Sipper Tubes and Filters ............................................................................................................... 40 6.1.2 Installing New Sipper Tubes and Filters .................................................................................................................. 42 6.2 Maintenance Wash .................................................................................................................................. 46 6.3 Test Sequencing Run with Control DNA Beads ................................................................................. 50 6.4 Pump Tubing and Air Filter Replacement............................................................................................ 50 6.4.1 Replacing the Pump Tubing ......................................................................................................................................... 51 6.4.2 Replacing the Air Filter ................................................................................................................................................... 57 6.5 Instrument Test ......................................................................................................................................... 57 6.6 Roche Preventive Maintenance ............................................................................................................ 63 6.7 Reconfiguring the emPCR Shaker Adapters MV for Older TissueLyser Models ........................ 63 June 2012 4 GS Junior Instrument Owner’s Manual 1 PREFACE 1.1 About this Manual The GS Junior Instrument Owner’s Manual briefly describes the hardware and software components of the GS Junior System, tells how to maintain the instrument, and provides guidelines for troubleshooting instrument problems. 1.2 GS Junior System Documentation Set The documentation set describing the GS Junior System comprises the documents listed in Table 1. A brief description of each document is given below. These are available electronically in printable format (PDF) as well as in e-manual format. The e-manual format offers the