Pierce’s Disease Control Program Symposium Proceedings 2006 Pierce’s Disease Research Symposium November 27-29, 2006 The Westin Horton Plaza San Diego California Department of Food & Agriculture Proceedings of the 2006 Pierce’s Disease Research Symposium November 27 – 29, 2006 Westin Horton Plaza Hotel San Diego, California Organized by: California Department of Food and Agriculture Chief Editor: Thomas Esser Compiling, Formatting, Proofreading, and Editing: M. Athar Tariq, Raygina Medeiros, Melinda Mochel, and Sean Veling Cover Photograph and Design: Sean Veling Printer: Copeland Printing, Sacramento, CA Funds for Printing Provided by: CDFA Pierce’s Disease and Glassy-winged Sharpshooter Board To Order Additional Copies of this Publication, Please Contact: Pierce’s Disease Control Program California Department of Food and Agriculture 1220 N Street, Room 325 Sacramento, California 95814 Telephone: (916) 651-0253 Fax: (916) 651-0275 http://www.cdfa.ca.gov/phpps/pdcp E-mail: [email protected] Cite as: Proceedings, 2006 Pierce’s Disease Research Symposium. California Department of Food and Agriculture, Sacramento, CA. History of Revisions to Electronic Version: 1. Nov. 8, 2006 -- Pages 174, 181, 182,and 306 revised. Taxonomic Note: A recent paper (see citation below) has prompted a change in the scientific name of the glassy-winged sharpshooter (GWSS). The scientific name Homalodisca coagulata (Say, 1832) has been replaced with the name Homalodisca vitripennis (Germar, 1821). Accordingly, this document uses Homalodisca vitripennis as the scientific name for GWSS. • Reference: Takiya, D.M., S.H. McKamey, & R.R. Cavichioli. 2006. Validity of Homalodisca and of H. vitripennis as the name for glassy-winged sharpshooter (Hemiptera: Cicadellidae: Cicadellinae). Annals Entomol. Soc. Amer. 99(4): 648-655. • Source: Serguei V. Triapitsyn (UC Riverside) and David J.W. Morgan (CDFA). Disclaimer: The reports in this publication have not been subject to scientific peer review. The California Department of Food and Agriculture (CDFA) makes no warranty, expressed or implied, and assumes no legal liability for the information in this publication. The publication of the Proceedings by CDFA does not constitute a recommendation or endorsement of products mentioned. TABLE OF CONTENTS Section 1: Vector Biology and Ecology Modeling Sharpshooter Transmission of Xylella fastidiosa Rodrigo Almeida ......................................................................................................................................................................... 3 Quantitative Aspects of the Transmission of Xylella fastidiosa by the Glassy-winged Sharpshooter Blake Bextine and Matthew J. Blua............................................................................................................................................. 4 Dispersal and Movement of the Glassy-winged Sharpshooter and Associated Natural Enemies in a Continuous, Deficit-Irrigated Agricultural Landscape Russell L. Groves, Marshall W. Johnson, and James Hagler....................................................................................................... 8 Spatial Population Dynamics and Overwintering Biology of the Glassy-winged Sharpshooter in California’s San Joaquin Valley Marshall W. Johnson, Kent Daane, Russell Groves, and Elaine Backus................................................................................... 12 Web Geographic Information Systems for Mapping Pierce’s Disease and Glassy-winged Sharpshooter in California Maggi Kelly............................................................................................................................................................................... 16 Analysis of the Bacterial Community Inhabiting Glassy-winged Sharpshooter Foregut by Culture-Dependent Techniques and DGGE Paulo T. Lacava, Francisco Dini-Andreote, Jose Luiz Ramirez, Jennifer Parker, Welington L. Araújo, João Lucio Azvedo, and Thomas A. Miller................................................................................................................................................................ 20 Species Diversity, Distribution, and Abundance of Xylem Fluid Feeding Hemiptera in Vineyards Throughout Texas Isabelle Lauzière and Forrest Mitchell ...................................................................................................................................... 23 Associative Learning of Host-Plant Chemical Stimuli in Immature Glassy-winged Sharpshooters Joseph M. Patt and Mamoudou Sétamou................................................................................................................................... 25 Evaluation of Blue-green Sharpshooter Flight Height Ed Weber................................................................................................................................................................................... 27 Abstract Only The Role of Olfactory Cues in Host-Plant Selection by the Glassy-Winged Sharpshooter Hannah Nadel ............................................................................................................................................................................ 30 Section 2: Vector Management Curtailing Oviposition by the Glassy-winged Sharpshooter on Nursery Plants Matthew J. Blua and Richard A. Redak..................................................................................................................................... 33 Potential Isolates of the Entomopathogenic Fungus Beauveria bassiana for Glassy-winged Sharpshooter Control Surendra K. Dara and Harry K. Kaya........................................................................................................................................ 36 - i - Genetic Characterization of Gonatocerus tuberculifemur from South America Uncovers Divergent Clades: Prospective Egg Parasitoid Candidate Agent for the Glassy-winged Sharpshooter in California Jesse H. de León, Guillermo A. Logarzo, and Serguei V. Triapitsyn ........................................................................................40 Preliminary Evidence from Reproductive Compatibility Studies Suggests That Gonatocerus tuberculifemur Exists as a Cryptic Species Complex, or a New Species Is Identified: Development and Utility of Molecular Diagnostic Markers Jesse H. de León, Guillermo A. Logarzo, and Serguei V. Triapitsyn ........................................................................................44 ISSR-PCR DNA Fingerprinting Uncovers Distinct Banding Patterns in Gonatocerus Species 3 Individuals Emerging from Different Host Tribes: A Prospective Egg Parasitoid Candidate Agent for the Glassy-winged Sharpshooter in California Jesse H. de León, Guillermo A. Logarzo, and Serguei V. Triapitsyn ........................................................................................48 Genetic Studies of Gonatocerus metanotalis Populations from Argentina Uncover Divergent Clades: A Prospective Egg Parasitoid Candidate Agent for the Glassy-winged Sharpshooter in California Jesse H. de León, Guillermo A. Logarzo, and Serguei V. Triapitsyn ........................................................................................52 The Utility of the Internal Transcribed Spacer Region 2 (ITS2) in Confirming Species Boundaries in the Genus Gonatocerus: Comparison to the Cytochrome Oxidase Subunit I Gene and Taxonomic Data: Molecular Key Based on ITS2 Sizes Jesse H. de León, Guillermo A. Logarzo, and Serguei V. Triapitsyn ........................................................................................56 Development and Utility of a ‘One-Step’ Species-Specific Molecular Diagnostic Marker for Gonatocerus morrilli Designed Toward the Internal Transcribed Spacer Region 2 to Monitor Establishment in California Jesse H. de León and David J.W. Morgan .................................................................................................................................60 Identifying Key Predators of the Glassy-winged Sharpshooter in a Citrus Orchard Valerie Fournier, James Hagler, Kent Daane, and Jesse de León..............................................................................................64 Exploration for Biological Control Agents in the Native Range of the Glassy-winged Sharpshooter John Goolsby .............................................................................................................................................................................67 An Immunological Approach for Quantifying Predation Rates on the Glassy-winged Sharpshooter James Hagler, Russell Groves, Marshall Johnson, and David Morgan......................................................................................70 Are Glassy-winged Sharpshooter Populations Regulated in California? Long-Term Phenological Studies and Construction of Multi-Cohort Life Tables for the Glassy-winged Sharpshooter in Citrus Orchards Mark S. Hoddle..........................................................................................................................................................................73 Determining the Day-Degree Requirements for Glassy-winged Sharpshooter Development and Quantification of Demographic Statistics at Five Temperatures Mark S. Hoddle..........................................................................................................................................................................76 Should Neoclassical Biological Control Agents from Argentina Be Released