Efficient Professional Protein and Antibody Platforms ALOX12 Antibody Basic information: Catalog No.: UPA60470 Source: Rabbit Size: 50ul/100ul Clonality: polyclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: affinity purified by Protein A Useful Information: Applications: WB:1:500-2000 Reactivity: Human, Mouse, Rat, Cow Specificity: This antibody recognizes ALOX12 protein. KLH conjugated synthetic peptide derived from human 12 Lipoxygenase Immunogen: 101-200/663 Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabo- lize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Description: Through the production of specific bioactive lipids like (12S)-HPETE it regu- lates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expres- sion of the vascular endothelial growth factor. Plays a role in apoptotic pro- cess, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. {ECO:0000269|PubMed:16638750, Uniprot: P18054 Human BiowMW: 76 KDa Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Sample: Raw264.7 Cell Lysate at 40 ug A431 Cell Lysate at 40 ug Primary: Anti-ALOX12 at 1/300 dilution Secondary: IRDye800CW Goat An- ti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kD Observed band size: 73 kD Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] .