neuroscience - Neurotransmission alphabetical 28/7/05 15:52 Page 1 Abcam’s range of Neuroscience receptor, channel and ligand antibodies includes over 320 tried and tested products www.abcam.com Neurotransmission - receptor and channel signaling www.abcam.com GPCRs TYROSINE KINASES ION CHANNELS Neurotrophins rin u BDNF rt NGF u NT-3 Glutamate GABA ACh GDNF Ne Artemin Persepin NT-4/5 Glutamate Anandamide ATP Glycine ␥ 2-AG 1 2 3 4 / ␣ ␣ ␣ ␣ R / u A GFR GFR GFR GFR NTR mGL X1, 2, 3 CB1/2 2 EphA/B* Ephrin RET TrkA/B/C p75 AMPA/KainateNMDAR P GABA GlyR nAChR IP3 released Lyn Ephexin Homer DAG Adenylate Fyn PI3K Gr Src PSD95 Rapsin i PD2RGS3 Shc Syntrophin b K PLC Gi/q cyclase Gi 4 NcK PI3 Shc + 2+ 2+ PI3K PLC ␥ Na Ca Ca P PI3K Grb2 PLC␤/␥ PKC nNOS ␥ FAK PiP3/4 SOS MEK CaM Cl- Cl- ER NFkB PDEI PKA Rac RhoA RasGAP Src PTEN PKC Ras GTPase ERK CaMK 2+ PKK2 Calcineurin Ca released ROCK ERK FRS2 AKT/PKB Raf JNK nNOS SynGAP Grb2 actin MEK 1/2 P Ser133 Inhibition Cl- Src SOS Cell survival CREB of signaling RAS P CREB ERK 1/2 P P MAPK GSK3 ELK RSK NF␬B CREB CREB gene Long term Cytoskeleton transcription synaptic dynamics, neurite ASK1 Bad CRE plasticity (LTP) P P extension CREB CREB Short term MKK3/6 Apoptosis plasticity Others Other GPCRs - ARA9 ACh (Muscarinic): M1, M2, M3, M4 CRE p38MAPK c-fos Potassium - ASIC␤, ASIC3 Adrenalin: Alpha1b, 1c, 1d - KChIP2 - DDR 1, 2 ATP: P2Y MSK1 c-j c-fos gene - KCNQ 3, 5 - ENSA Dopamine: D1, D4 u transcription - Kv beta 2 - GJB1 n GABA: GABAB receptor - Kv 1.2 - SLC31A1 ␦ ␬ P P Opioid: µ, , CREB CREB AP1 - Kv 1.3 - TRAR4 Neurotensin: 1, 2 Growth, - Kv 1.4 - TRPM7 Neurokinin: NKA, NKB, NK1, Calcitonin development & Sodium - Kv 2.2 - VR1 * forward/reverse signalling differentation Serotonin: 5HT-1B, D, E, F, 5HT-2A, B, 5HT-3, 4, 6, 7 inhibitory pathway CRE - APXL - Kv 4.2 - VRL1 neuroscience - Neurotransmission alphabetical 28/7/05 15:52 Page 2 Browse our datasheets for the latest customer reviews and enquires on neurotransmission products! www.abcam.com Target Datasheet Clonality Applications Neurotensin Receptor 2 13069 P IHC-P Kv2.2 (azide free) 21909 P IHC, WB www.abcam.com/ab tested NPY5R 13199 P ICC, IHC-P Kv4.2 16719 P IF, IP, WB P Y12 13373 P ICC, IHC-P Cannabinoid & Dopamine 2 more ion channels Cannabinoid Receptor I 10573 P IF, IHC-Fr, WB P2Y2 10270 P IHC-Fr Somatostatin Receptor 2 13120 P IF, IHC-P 5HT3 Receptor 13897 P WB Cannabinoid Receptor II 3560 P ICC, WB alpha 1 Glycine Receptor 475 P IP, WB Dopamine Receptor D1 12969 P ICC, IHC-P Tyrosine Kinases ARA9 [35-2] 468 M WB Dopamine Receptor D4 13318 P IHC-P EphA1 5376 P ELISA, WB DDR1 5508 P ELISA, WB Glutamate EphA2 5386 P ELISA, WB DDR2 5520 P ELISA, WB Homer (1a+1b+1c) 11157 P IHC-Fr, WB EphB1 7042 ELISA, WB KA1 10101 P WB GRM8 13194 P IHC-P EphB2 5418 P ELISA, WB P2X2 10266 P ICC, IHC-Fr, WB mGluR1a 6439 PIF, IHC, IHC-Fr, IP, WB EphB3 10617 P ELISA, WB P2X3 10269 P ICC, IHC-Fr, WB mGluR2 [mG2Na-s] 15672 M IF, IHC, WB Fyn 802 P ELISA, WB TRPM7 729 P WB ␣ mGluR2+3 6438 P IHC, IP, WB GFR 3 19135 P WB Vanilloid Receptor 1 6166 P IF, IHC-Fr, IHC-P Lyn 1959 P IF, IP, WB mGluR3 10309 P WB KEY: M - Monoclonal P - Polyclonal mGluR5 6436 P IHC, IP, WB p75 NTR 10495 P EM, FACS,ICC, IF, IP, WB mGluR7 13363 P IHC-P TrkA 8871 P IF, IHC, IP, trkA inact. WB TrkB 6180 P IHC-Fr Cannabinoid Receptor I antibody (ab10573) Opioid & Seratonin Cannabinoid receptor 1 (CB1) inhibits adenylate cyclase activity in a 5-HT1B 13154 P IHC-P GABA receptors ␣ dose dependent, stereoselective and pertussis toxin sensitive manner. 5HT2B 13292 P IHC-P GABA A Receptor 1 8341 P WB ␣ CB1 also stimulates the mitogen-activated protein kinase and 5HT3 13897 P WB GABA A Receptor 2 8342 P WB modulates ion channels through direct G-protein interactions. ␤ 5-HT4 10095 P WB GABA A Receptor 2 8340 P IP, WB ␦ CB1 is primarily expressed in the brain. 5HT6 13293 P IHC-P GABA A Receptor 10100 P WB ␥ ab10573 shows localisation in neurons Delta Opioid Receptor 10272 P ICC GABA A Receptor 2 4073 P IP, WB GABA B Receptor 2 1134 P WB of the arcuate nucleus of rat brain Kappa Opioid Receptor 10283 P ICC, IHC-Fr hypothalamus (shown here). CB1 is also Mu Opioid Receptor 10275 P ICC, IHC-Fr, WB nAch receptors expressed at low level in peripheral tissues including adrenal, heart, lung, prostate, Neurotrophins nAChR [88B] 2804 M IHC-Fr, IP, WB ␣ uterus, ovary, testis, bone marrow, thymus, Artemin 1136 P WB nAChR [D6] 11149 M FACS, IHC-Fr, IP, WB ␤ and tonsil. BDNF 6201 P ELISA, IHC-Fr, Neut, WB nAChR [B3] 11150 M FACS, IHC-Fr, IP, WB ␥ GDNF 6206 P Dot, IHC-Fr, WB nAChR [C9] 11151 M FACS, IHC-Fr, IP, WB Applications tested for ab10573: Neurturin 6208 P Dot, ELISA, IHC-Fr, WB Rapsyn [1234] 11423 M ICC, IF, IHC-Fr, WB G western blot G immunofluorescence G immunohistochemistry NGF 6198 P ELISA, IHC-Fr, Neut, WB Syntrophin [1351] 11425 M IF, IP, WB Mu Opioid Receptor antibody (ab10275) other GPCRs NMDA receptors NMDAR1 C1 6484 P IHC, IP, WB ␣1d Adrenergic Receptor 12923 P IHC-P Three major opioid receptor families have now been proposed: mu, NMDAR1 [R1JHL] 1880 M WB Calcitonin Receptor 11042 P ELISA, IHC-P, WB kappa and delta. Mu Opioid Receptor (MOR) plays a mandatory role in NMDAR1 N1 6483 P IHC, IP, WB CCKBR 14440 P IHC-P, WB the analgesic effects of opioids and most of the opioid analgesics used NMDAR2A 108 P AF, IHC-F, IHC-Fr, IP, WB Corticotropin R F Receptor 12964 P IF, IHC-Fr, IHC-P, WB clinically today selectively bind to MOR sub types. NMDAR2B 109 P AF, IHC-F, IHC-Fr, IP, WB EDG7 13188 P IHC-P NMDAR2C 110 P IHC, IP, WB MOR is expressed by neurons and glia and GLP1R 13181 P IHC-P is enriched in many brain areas including HRH3 13014 P ICC, IHC-P Sodium and potassium channels cerebral cortex, striatum (shown here MAChR 1 3480 P WB APXL 4948 P Fast track labelled with ab10275), hippocampus, MAChR M3 13064 P IHC-P ASIC3 10354 P ICC, IHC-Fr locus coeruleus and the superficial laminae of the dorsal horn. MC4 Receptor 13034 P ICC, IHC-Fr, IHC-P KChIP2 3473 P WB Melatonin Receptor 1A 13036 P IHC-P KCNQ3 16228 P ICC, IF, IHC-Fr Applications tested for ab10275: Melatonin Receptor 1B 13357 P IHC-P Kv␤2 10665 P ICC, WB G western blot G immunocytochemistry G immunohistochemistry (frozen sections) Neurokinin A Receptor 13397 P ICC, IHC-P Kv1.2 10667 P ICC, WB Neurotensin Receptor 1 13201 P IHC-P Kv1.4 16718 P IF, IP, WB www.abcam.com/neuroscience.