Supplementary Table 1: Genes underexpressed in wt-ACVR2-transfected cells grouped according to their biological function. SAM Fold Description Accession score change Enzyme activity CA9 Renal cell carcinoma associated antigen G250 NM001216 -4.44 5.34 LDHA Lactate dehydrogenase A NM005566 -4.42 3.30 ASL Argininosuccinate lyase NM000048 -3.27 2.16 Branched chain keto acid dehydrogenase E1, alpha -3.16 BCKDHA NM000709 2.49 polypeptide (maple syrup urine disease) BDH 3-hydroxybutyrate dehydrogenase NM004051 -3.16 2.31 ALDOA Aldolase A, fructose-bisphosphate NM000034 -3.03 2.82 UREB1 Upstream regulatory element binding protein 1 AB071605 -2.97 1.61 SPS Selenophosphatase synthetase NM012247 -2.87 1.68 GSTM3 Glutathione S-transferase M3 NM000849 -2.80 2.53 HPCL2 2-hydroxyphytanoyl-CoA lyase XM011020 -2.73 1.58 UQCR Ubiquinol-cytochrome c reductase NM006830 -2.69 2.16 DNA replication RRM2 Ribonucleotide reductase M2 polypeptide NM001034 -4.10 2.29 Regulation of transcription ENO1 Alpha enolase NM001428 -2.89 2.19 Signal transduction MAP2K5 Mitogen-activated protein kinase kinase 5 NM002757 -3.77 1.88 MAPK13 Mitogen-activated protein kinase p38delta NM002754 -2.99 2.08 MAPK3 Mitogen-activated protein kinase 3 NM002746 -2.82 1.84 Regulation of cell cycle HDAC3 Histone deacetylase 3 NM003883 -2.97 1.72 Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase -3.43 YWHAQ NM006826 1.67 activation protein, theta polypeptide RNA binding PCBP2 Poly(rC)-binding protein 2 AK023529 -3.32 2.01 Cell adhesion LAMR1 Laminin receptor 1 NM002295 3.92 2.06 Anti-apoptosis BIRC5 Baculoviral IAP repeat-containing 5 (survivin) NM001168 3.71 1.88 Actin binding VIL1 Villin 1 NM007127 -3.69 4.95 CAPZA2 Capping protein (actin filament) muscle Z-line, alpha 2 NM006136 -2.73 1.57 Control of actin polymerization ARPC4 Actin related protein 2/3 complex, subunit 4 (20 kD) NM005718 -3.49 2.20 Metabolism PEC1 Peroxisomal D3,D2-enoyl-CoA isomerase NM006117 -3.72 2.21 Antioxidant PRDX3 Peroxiredoxin 3 NM006793 -3.29 1.86 PRDX2 Peroxiredoxin 2 NM005809 -3.72 1.96 Transport ACTR1A Actin-related protein. NM005736 -3.04 1.92 SLC16A5 Putative monocarboxylate transporter MCT NM004695 -2.89 1.67 SLC25A5 Solute carrier family 25 NM001152 -2.75 2.00 AP2B1 Adaptor-related protein complex 2, beta 1 subunit NM001282 -2.69 1.75 Others DXS1283E GS2 gene NM004650 -4.08 1.98 E46L Like mouse brain protein E46 NM013236 -3.43 1.90 RPS27A Ribosomal protein S27a NM002954 -3.25 1.61 MRPS27 Mitochondrial ribosomal protein S27 NM015084 -3.25 1.60 SERPINB1 Serine (or cysteine) proteinase inhibitor, clade B NM030666 -3.09 4.05 ITM2A Integral membrane protein 2A NM004867 -3.10 1.99 ARL7 ADP-ribosylation factor 7 NM005737 -2.82 2.72 ARL3 ADP-ribosylation factor-like 3 XM011921 -2.79 1.77 Fold Change is the average of the relative expression ratios for the three wt-ACVR2-transfectants to the reference probe divided by the average of the relative expression ratios for the three vector control transfectants to the reference probe. Genes in red have previously been reported to be related to TGF-β signaling. (23-26, 31) .