4th International Clostridium difficile Symposium Abstract book ©2012, Bled, Slovenia Zbrali in uredili: Maja Rupnik Sandra Janežič Oblikovanje in prelom: Uroš Zupančič Založil: Zavod za zdravstveno varstvo Maribor Naklada: 300 kos Organizing committee Maja Rupnik (Maribor, Slovenia) Anne Collignon (Paris, France) Michel Delmee (Brussels, Belgium) Bruno Dupuy (Paris, France) Dale Gerding (Chicago, USA) Haru Kato (Tokyo, Japan) Tom Louie (Calgary, Canada) Paola Mastrantonio (Rome, Italy) Nigel Minton (Nottingham, UK) Ian Poxton (Edinburg, UK) Tom Riley (Perth, Australia) Bo-Moon Shin (Seoul, Korea) Glenn Songer (Ames, USA) Linc Sonenshein (Boston, USA) Scott Weese (Guelph, Canada) Vse pravice pridržane. Noben del te izdaje ne sme biti reproduciran, shranjen ali prepisan v katerikoli obliki oz. na katerikoli način, bodisi elektronsko, mehansko, s fotokopiranjem, snemanjem ali kako drugače, brez predhodnega privoljenja založnika. CIP - Kataložni zapis o publikaciji Narodna in univerzitetna knjižnica, Ljubljana 579.852.13(082) 616.98:579.852.13(082) INTERNATIONAL Clostridium Difficile Symposium (4 ; 2012 ; Bled) Abstract book / 4th ICDS - International Clostridium Difficile Symposium, September, 20th to 22nd 2012, Bled, Slovenia ; [zbrali in uredili Maja Rupnik, Sandra Janežič]. - Maribor : Zavod za zdravstveno varstvo, 2012 ISBN 978-961-90895-6-9 1. Rupnik, Maja, 1967- 263192832 ICDS would like to acnowledge the contributions of CONTENTS 7 Welcome 9 General Information 11 Invited Speakers 15 Attendance Grants 19 ICDS Programme 27 Abstracts of oral presentations 71 Abstracts of poster presentations (section 1 P1 to P62, Friday) 139 Abstracts of poster presentations (section 2 P63 to P120, Saturday) 207 ICDS Participants 5 WELCOME Dear friends and colleagues, It is a great pleasure to welcome you to the Fourth International Clostridium difficile Symposium. Clostridium difficile continues to be one of the most important human health-care associated pathogens, and its role in community-associated infections and in animal disease is being more widely recognised. Following some success in preventing the infection in parts of the world where Clostridium difficile infection (CDI) was unacceptably high, it has begun to decline. However, in other parts of the world it is still increasing, or just being recognised as a problem. We hope that the programme of the 4th ICDS reflects the range of research that is currently addressing the main biological, clinical and veterinary issues of CDIs. The major targets remain the prevention, epidemiology, control and diagnosis of disease, especially of recurrent infections and of outbreaks. The importance of the host has long been recognised as crucial in the susceptibility to infection and research emphasis on pathogenesis and immunology are increasing. Similarly, spores, the infectious agent in CDI are also being investigated in much more detail. Major advances in the genetics of C. difficile have been made, with genetic manipulation becoming generally available and many genome sequences have now been published. We hope, by the end of the meeting participants will be fully updated in all important areas of C. difficile research. ICDS is established as the traditional C. difficile meeting internationally and the venue is also traditionally located in Slovenia. Following the popularity of Bled in 2010, we are here again (Kranjska Gora in 2004; Maribor 2007). The Organizing Committee hopes that you will enjoy the meeting and that it will stimulate new ideas and new collaborations. On behalf of the Organizing Committee Maja Rupnik 7 GENERAL INFORMATION Congress venue Hotel Golf, Bled, Slovenia Registration and information desk opening hours Wedneday 19th September 17.00 to 18.30 Thursday 20th September 10.00 to 14.30 Friday 21st September 8.00 to 8.30 and during lunch breaks and coffee breaks Saturday 22nd September 8.00 to 8.30 and during lunch breaks and coffee breaks Meals and Social Events Lunch on Friday, 21st September, and Saturday, 22nd September and Dinner Friday, 21st September are included in the Registration. Meals will be served in the Restaurant of the Hotel Golf. Coffee Breaks will be served in front of the lecture hall and after the lunch in the poster area. Welcome reception, Thursday, 20th September, is included in Registration and will be held in the area in front of Hall Jupiter. Congress dinner, Saturday, 22nd September, is included in Registration and will be held in Grand Hotel Toplice. Concert On Wednesday, 19th September a half hour concert of Zither duo Fantasia is organized in Hotel Golf, Hall Jupiter at 18.30. Admission is free. Badges Name badges are requested during all congress scientific sessions as well as during social events. For Speakers Speakers are kindly requested to upload their presentation to the computer in the lecture hall. Technical assistance will be provided. Use of own computers is not encouraged. For poster presenters Posters will be displayed in two sessions. Distribution into the sessions is given in the Poster overview table. Posters for Session I can be displayed from Thursday, 20th September morning and should be removed on Friday 21st September afternoon coffee break (16.30 to 17.00). Posters for Session II should be displayed from Friday 21st September afternoon coffee break (16.30 to 17.00) and removed after the last session on Saturday 22nd September. 9 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM 4th International Clostridium difficile Symposium Invited Speakers 11 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM Abstracts of poster presentations 12 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM Invited speakers INVITED SPEAKERS Carroll Karen (Johns Hopkins University School of Medicine, Baltimore, USA) Dingle Kate (Oxford University, Oxford, United Kingdom) Donskey Curtis (Cleveland VA Medical Center, Cleveland, USA) Henriques Adriano (Instituto de Tecnologia Química e Biológica, Oeiras, Portugal) Indra Alexander (AGES-IMED, Vienna, Austria) Kuijper Ed (Leiden University Centre, Leiden, The Netherlands) Lacy Borden (Vanderbilt University School of Medicine, Nashville, USA) Mulvey Michael (Public Health Agency of Canada, Winnipeg, Canada) Ross Paul (Teagasc, Fermoy, Ireland) Sansonetti Philippe (Institut Pasteur / College de France, Paris, France) Sonenshein Linc (Tufts University School of Medicine, Boston, USA) Wilcox Mark (Leeds Teaching Hosptials/University of Leeds, Leeds, United Kingdom) 13 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM 4th International Clostridium difficile Symposium Attendance Grants 15 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM Abstracts of poster presentations 16 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM Attendance grants The ESCMID Attendance grants Chilton Caroline (University of Leeds, Leeds, United Kingdom) Crowther Grace (University of Leeds, Leeds, United Kingdom) Di Bella Stefano (National Institute for Infectious Diseases "L. Spallanzani", Roma, Italy) Donahue Elizabeth (London School of Hygiene and Tropical Medicine, London, United Kingdom) El Meouche Imane (GRAM Laboratory, University of Rouen, Rouen, France) Khanafer Nagham (Lyon 1 University- Edouard Herriot Hospital, Lyon, France) Miyajima Fabio (University of Liverpool, Liverpool, United Kingdom) Saujet Laure (Pasteur Institute, Paris, France) Seager Zoe (Imperial College London, London, United Kingdom) Shaw Helen Alexandra (Imperial College London, London, United Kingdom) Wasels François (Istituto Superiore di Sanità, Rome, Italy) Williams Rachel (UCL Eastman Dental Institute, London, United Kingdom) Willing Stephanie (Imperial College London, London, United Kingdom) The ESCMID Study Group for Clostridium difficile/ICDS grants Cafardi Valeria (University of Rome "La Sapienza", Siena, Italy) Dobreva Elina (National Centre Of Infectious and Parasitic Diseases, Sofia, Bulgaria) Jovanovic Milica (Clinic for Infectious and Tropical Diseases of Clinical Center of Serbia, Belgrade, Serbia) Mentula Silja (National Institute for Health and Welfare (THL), Helsinki, Finland) Orfanidou Maria (General Hospital of Athens G. Gennimatas, Ekali , Greece) Papatheodorou Panagiotis (Albert-Ludwigs-Universität Freiburg, Freiburg, Germany) Pereira Fatima (ITQB-UNL, Lisboa, Portugal) Romano Vincenza (Parthenope University of Naples, Naples, Italy) van Eijk Erika (Leiden University Medical Center, Leiden, The Netherlands) 17 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM Attendance grants The ICDS grants Ammam Fariza (Paris Sud 11, Chatenay Malabry, France) Bahadur Tej (All India Institute of Medical Sciences, New Delhi, India) Ghose-Paul Chandrabali (Aaron Diamond AIDS Research Center, New York, USA) Moura Ines (Istituto Superiore di Sanità, Rome, Italy) Pecavar Verena (AGES-IMED, Vienna, Austria) Rodriguez Diaz Cristina (University of Liège Faculty of Veterinary Medicine, Liège, Belgium) Senoh Mitsutoshi (National Institute of Infectious Diseases, Musashimurayama, Japan) Sijoria Richa (Defense Research and Development Establishment, Gwalior, India) Tagashira Yasuaki (Asahi General Hospital, Fuchu-city, Japan) Taori Surabhi (University of Edinburgh, Edinburgh, United Kingdom) 18 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM 4th International Clostridium difficile Symposium ICDS Programme 19 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM Abstracts of poster presentations 20 4th INTERNATIONAL CLOSTRIDIUM DIFFICILE SYMPOSIUM ICDS Programme Thursday, 20th September 14:45-16:30 SESSION I: IMMUNOLOGY I Chair: P. Mastrantonio 14:45 Rupnik, M. OPENING 15:00 INV1 Sansonetti,